A potential germ cell-specific marker in Japanese flounder, <italic style="font-style: italic">Paralichthys olivaceus</italic>: identification and characterization of lymphocyte antigen 75 (Ly75/CD205)

杨阳; 刘清华; 马道远; 宋宗诚; 李军

doi:10.1007/s00343-019-7390-z

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A potential germ cell-specific marker in Japanese flounder, Paralichthys olivaceus: identification and characterization of lymphocyte antigen 75 (Ly75/CD205)

Biology | Updated：2023-05-19

- A potential germ cell-specific marker in Japanese flounder, Paralichthys olivaceus: identification and characterization of lymphocyte antigen 75 (Ly75/CD205)
- A potential germ cell-specific marker in Japanese flounder, Paralichthys olivaceus: identification and characterization of lymphocyte antigen 75 (Ly75/CD205)
- 海洋湖沼学报（英文） 2018年36卷第4期页码：1342-1348
- Affiliations：
  
  1.Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China
  2.Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071, China
  3.University of Chinese Academy of Sciences, Beijing 100049, China
  4.Weihai Shenghang Aquatic Product Science and Technology Co. Ltd., Weihai 264200, China
- Author bio：
  
  LIU Qinghua, qinghualiu@qdio.ac.cn
  LI Jun, junli@qdio.ac.cn
- Funds：
  
  the National Natural Science Foundation of China(31372514);the National Natural Science Foundation of China(31472264);the National Natural Science Foundation of China(31572602);the China Agriculture Research System(CARS-47);the Aoshan Technology Innovation Program of Qingdao National Laboratory for Marine Science and Technology(2015ASKJ02);the Scientific and Technological Innovation Project Financially Supported by Qingdao National Laboratory for Marine Science and Technology(2015ASKJ02-03-03);the Youth Innovation Promotion Association CAS and Chinese Academy of Science and Technology Service Network Planning(KFJ-EW-STS-060)
- DOI：10.1007/s00343-019-7390-z
  中图分类号：
- 收稿：2017-12-19，
  
  录用：2018-2-22，
  
  网络首发：2017-02-27，
  
  纸质出版：2018-07
- Accepted：
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杨阳, 刘清华, 马道远, 等. A potential germ cell-specific marker in Japanese flounder, Paralichthys olivaceus: identification and characterization of lymphocyte antigen 75 (Ly75/CD205)[J]. 海洋湖沼学报（英文）, 2018,36(4):1342-1348.

Yang YANG, Qinghua LIU, Daoyuan MA, et al. A potential germ cell-specific marker in Japanese flounder, Paralichthys olivaceus: identification and characterization of lymphocyte antigen 75 (Ly75/CD205)[J]. Chinese Journal of Oceanology and Limnology, 2018, 36(4): 1342-1348.
杨阳, 刘清华, 马道远, 等. A potential germ cell-specific marker in Japanese flounder, Paralichthys olivaceus: identification and characterization of lymphocyte antigen 75 (Ly75/CD205)[J]. 海洋湖沼学报（英文）, 2018,36(4):1342-1348. DOI： 10.1007/s00343-019-7390-z.

Yang YANG, Qinghua LIU, Daoyuan MA, et al. A potential germ cell-specific marker in Japanese flounder, Paralichthys olivaceus: identification and characterization of lymphocyte antigen 75 (Ly75/CD205)[J]. Chinese Journal of Oceanology and Limnology, 2018, 36(4): 1342-1348. DOI： 10.1007/s00343-019-7390-z.

摘要

Abstract

Some germ cell marker genes

such as

vasa

nanos

and

dead end

(

dnd

)

have been identified in fish. Recently

lymphocyte antigen 75 (Ly75/CD205) has been identified as a mitotic germ cell-specific cell-surface marker in several fish species. In this study

the Japanese flounder

Paralichthys olivaceus

ly75 homolog (

ly75

) was cloned and its expression pattern in gonads was analyzed. The full-length cDNA of

ly75

was 7 346 bp

with an open reading frame (ORF) of 5 229 bp. The ORF encoded a protein containing 1 742 amino acids with a predicted molecular mass of 196.89 kDa. In adult tissues

ly75

transcripts were detected in all analyzed tissues but abundantly in the testis. In in-situ hybridization analyses

ly75

mRNA was predominantly localized in oocytes in the ovary and spermatogonia in the testis

but

ly75

mRNA was not detected in oogonia

spermatocytes

spermatids

or spermatozoa. These results indicated that

ly75

could be a potential germ cell-specific marker in

P. olivaceus

as in other fishes.

关键词

Keywords

references

East L, Isacke C M. 2002. The mannose receptor family.Biochimica et Biophysica Acta (BBA)-General Subjects, 1572(2-3):364-386.

Fan Z F, You F, Wang L J, Weng S D, Wu Z H, Hu J W, Zou Y X, Tan X G, Zhang P J. 2014. Gonadal transcriptome analysis of male and female olive flounder ( Paralichthys olivaceus ). BioMed Research International, 2014:291067.

Huang J Q, Li Y J, Shao C W, Wang N, Chen S L. 2017.Identification, characterization and functional analysis of regulatory region of nanos gene from half-smooth tongue sole ( Cynoglossus semilaevis ). Gene, 617:8-16.

Jiang W P, Swiggard W J, Heufler C, Peng M, Mirza A, Steinman R M, Nussenzweig M C. 1995. The receptor DEC-205 expressed by dendritic cells and thymic epithelial cells is involved in antigen processing. Nature, 375(6527):151-155.

Kagawa H. 2013. Oogenesis in teleost fish. Aqua-BioScience Monographs (ABSM), 6(4):99-127.

Kurokawa H, Saito D, Nakamura S, Katoh-Fukui Y, Ohta K, Baba T, Morohashi K, Tanaka M. 2007. Germ cells are essential for sexual dimorphism in the medaka gonad.Proceedings of the National Academy of Sciences of the United States of America, 104(43):16 958-16 963.

Lacerda S M S N, Aponte P M, Costa G M J, Campos-Junior P H A, Segatelli T M, Silva M A, Franca L R. 2012. An overview of spermatogonial stem cell physiology, niche and transplantation in fish. Animal Reproduction, 9(4):798-808.

Li M J, Tan X G, Jiao S, Wang Q, Wu Z H, You F, Zou Y X. 2015. A new pattern of primordial germ cell migration in olive flounder ( Paralichthys olivaceus ) identified using nanos3 . Development Genes and Evolution, 225(4):195-206.

Nagasawa K, Fernandes J M O, Yoshizaki G, Miwa M, Babiak I. 2013. Identification and migration of primordial germ cells in Atlantic salmon, Salmo salar :characterization of Vasa , Dead End , and Lymphocyte antigen 75 genes . Molecular Reproduction and Development, 80(2):118-131.

Nagasawa K, Miwa M, Yazawa R, Morita T, Takeuchi Y, Yoshizaki G. 2012. Characterization of lymphocyte antigen 75 (Ly75/CD205) as a potential cell-surface marker on spermatogonia in Pacific bluefin tuna Thunnus orientalis . Fisheries Science, 78(4):791-800.

Nagasawa K, Shikina S, Takeuchi Y, Yoshizaki G. 2010.Lymphocyte antigen 75 (Ly75/CD205) is a surface marker on mitotic germ cells in rainbow trout. Biology of Reproduction, 83(4):597-606.

Nakamura S, Kobayashi K, Nishimura T, Tanaka M. 2011.Ovarian germline stem cells in the teleost fish, medaka( Oryzias latipes ). International Journal of Biological Sciences, 7(4):403-409.

Presslauer C, Nagasawa K, Dahle D, Babiak J, Fernandes J M O, Babiak I. 2014. Induced autoimmunity against gonadal proteins affects gonadal development in juvenile zebrafish.PLoS One, 9(12):e114209, https://doi.org/10.1371/journal.pone.0114209.

Radonic M, Macchi G J. 2009. Gonadal sex differentiation in cultured juvenile flounder, Paralichthys orbignyanus (Valenciennes, 1839). Journal of the World Aquaculture Society, 40(1):129-133.

Schulz R W, de França L R, Lareyre J J, Le Gac F, ChiariniGarcia H, Nobrega R H, Miura T. 2010. Spermatogenesis in fish. General and Comparative Endocrinology, 165(3):390-411.

Si Y F, Ding Y X, He F, Wen H S, Li J F, Zhao J L, Huang Z J. 2016. DNA methylation level of cyp19a1a and Foxl2 gene related to their expression patterns and reproduction traits during ovary development stages of Japanese flounder( Paralichthys olivaceus ). Gene, 575(2):321-330.

Suzuki A, Tsuda M, Saga Y. 2007. Functional redundancy among Nanos proteins and a distinct role of Nanos2 during male germ cell development. Development, 134(1):77-83.

Wang L J, You F, Weng S D, Wen A Y, Wu Z H, Zou Y X, Xin M J, Zhang P J. 2015a. Molecular cloning and sexually dimorphic expression patterns of nr0b1 and nr5a2 in olive flounder, Paralichthys olivaceus. Development Genes and Evolution, 225(2):95-104.

Wang P, Jiang C Y, Liu S S, Cui P F, Zhang Y, Zhang S C. 2017.Trans-generational enhancement of C-type lysozyme level in eggs of zebrafish by dietary β-glucan.Developmental & Comparative Immunology, 74:25-31.

Wang X Y, Liu Q H, Xiao Y S, Yang Y, Wang Y F, Song Z C, You F, An H, Xiao Z Z, Xu S H, Ma D Y, Li J. 2015b. The dnd RNA identifies germ cell origin and migration in olive flounder ( Paralichthys olivaceus ). BioMed Research International, 2015:428591.

Zhang S C, Wang Z P, Wang H M. 2013. Maternal immunity in fish. Developmental & Comparative Immunology, 39(1-2):72-78.

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A potential germ cell-specific marker in Japanese flounder, Paralichthys olivaceus: identification and characterization of lymphocyte antigen 75 (Ly75/CD205)

A potential germ cell-specific marker in Japanese flounder, Paralichthys olivaceus: identification and characterization of lymphocyte antigen 75 (Ly75/CD205)

DOI：10.1007/s00343-019-7390-z

摘要

Abstract

关键词

Keywords

references