The effects of electrochemical oxidation on in-vivo fluorescence and toxin content in <italic style="font-style: italic">Microcystis aeruginosa</italic> culture

Tinkara ROZINA; Tina ELERŠEK; Maja ZUPANČIČ JUSTIN; Andrej MEGLIČ; Domen LEŠTAN; Bojan SEDMAK

doi:10.1007/s00343-019-7180-7

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The effects of electrochemical oxidation on in-vivo fluorescence and toxin content in Microcystis aeruginosa culture

ICTC-10 Special Issue: Cyanobacteria and cyanotoxins: responses and detection | Updated：2023-05-19

- The effects of electrochemical oxidation on in-vivo fluorescence and toxin content in Microcystis aeruginosa culture
- The effects of electrochemical oxidation on in-vivo fluorescence and toxin content in Microcystis aeruginosa culture
- 海洋湖沼学报（英文） 2018年36卷第4期页码：1091-1102
- Affiliations：
  
  1.Envit d. o. o., Vojkova cesta 63, 1000 Ljubljana, Slovenia
  2.Department of Genetic Toxicology and Cancer Biology, National Institute of Biology, Večna pot 111, 1000 Ljubljana, Slovenia
  3.Arhel d. o. o., Pustovrhova ulica 15, 1000 Ljubljana, Slovenia
  4.Biotechnical Faculty, University of Ljubljana, Večna pot 111, 1000 Ljubljana
  5.Centre for Soil and Environmental Sciences, Biotechnical Faculty, University of Ljubljana, Jamnikarjeva 101, 1000 Ljubljana, Slovenia
- Author bio：
  
  Maja ZUPANČIČ JUSTIN, maja.justin@arhel.si
- Funds：
  
  the ARRS Project L1-5456 (Control of Harmful Cyanobacteria Bloom in Fresh-Water Bodies) and LIFE + Project:LIFE12 ENV/SI/00083 LIFE Stop CyanoBloom
- DOI：10.1007/s00343-019-7180-7
  中图分类号：
- 收稿：2017-06-15，
  
  录用：2017-12-6，
  
  网络首发：2017-12-18，
  
  纸质出版：2018-07
- Accepted：
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The effects of electrochemical oxidation on in-vivo fluorescence and toxin content in Microcystis aeruginosa culture[J]. 海洋湖沼学报（英文）, 2018,36(4):1091-1102.

Tinkara ROZINA, Tina ELERŠEK, Maja ZUPANČIČ JUSTIN, et al. The effects of electrochemical oxidation on in-vivo fluorescence and toxin content in Microcystis aeruginosa culture[J]. Chinese Journal of Oceanology and Limnology, 2018, 36(4): 1091-1102.
The effects of electrochemical oxidation on in-vivo fluorescence and toxin content in Microcystis aeruginosa culture[J]. 海洋湖沼学报（英文）, 2018,36(4):1091-1102. DOI： 10.1007/s00343-019-7180-7.

Tinkara ROZINA, Tina ELERŠEK, Maja ZUPANČIČ JUSTIN, et al. The effects of electrochemical oxidation on in-vivo fluorescence and toxin content in Microcystis aeruginosa culture[J]. Chinese Journal of Oceanology and Limnology, 2018, 36(4): 1091-1102. DOI： 10.1007/s00343-019-7180-7.

摘要

Abstract

The increasing occurrence of cyanobacterial blooms in water bodies is a serious threat to the environment. Efficient in-lake treatment methods for the control of cyanobacteria proliferation are needed

their in-vivo detection to obtain a real-time response to their presence

as well as the information about their physiological state after the applied treatment. In-vivo fluorescence measurements of photosynthetic pigments have proved to be effective for quantitative and qualitative detection of phytoplankton in a water environment. In the experiment

chlorophyll and phycocyanin fluorescence sensors were used concurrently to detect stress caused by electrochemical oxidation applying an electrolytic cell equipped with borondoped diamond electrodes on a laboratory culture of cyanobacteria

Microcystis aeruginosa

PCC 7806. The inflicted injuries were reflected in a clear transient increase in the phycocyanin fluorescence signal (for 104%±43%) 24 h after the treatment

which was not the case for the chlorophyll fluorescence signal. In the next 72 h of observation

the fluorescence signals decreased (on 40% of the starting signal) indicating a reduction of cell number

which was confirmed by cell count (24% reduction of the starting concentration) and analysis of extracted chlorophyll and phycocyanin pigment. These results demonstrate the viability of the combined application of two sensors as a useful tool for in-vivo detection of induced stress

providing real-time information needed for the evaluation of the efficiency of the in-lake treatment and decision upon the necessity of its repetition. The electrochemical treatment also resulted in a lower free microcystins concentration compared to control.

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references

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DOI：10.1007/s00343-019-7180-7

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