Comparison of intestinal microbiota and activities of digestive and immune-related enzymes of sea cucumber <italic style="font-style: italic">Apostichopus japonicus</italic> in two habitats

王琦; 张秀梅; 陈慕雁; 李文涛; 张沛东

doi:10.1007/s00343-018-7075-z

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Comparison of intestinal microbiota and activities of digestive and immune-related enzymes of sea cucumber Apostichopus japonicus in two habitats

Aquaculture and Fisheries | Updated：2023-05-19

- Comparison of intestinal microbiota and activities of digestive and immune-related enzymes of sea cucumber Apostichopus japonicus in two habitats
- Comparison of intestinal microbiota and activities of digestive and immune-related enzymes of sea cucumber Apostichopus japonicus in two habitats
- 海洋湖沼学报（英文） 2018年36卷第3期页码：990-1001
- Affiliations：
  
  1.Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, Qingdao 266003, China
  2.Laboratory for Marine Fisheries and Aquaculture, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266072, China
- Author bio：
  
  ZHANG Xiumei, xiumei1227@163.com
- Funds：
  
  the National Marine Public Welfare Research Project(201305043);the National Natural Science Foundation of China(31472257);the National Natural Science Foundation of China(41576112)
- DOI：10.1007/s00343-018-7075-z
  中图分类号：
- 收稿：2017-03-13，
  
  录用：2017-3-31，
  
  网络首发：2017-05-09，
  
  纸质出版：2018-05
- Accepted：
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王琦, 张秀梅, 陈慕雁, 等. Comparison of intestinal microbiota and activities of digestive and immune-related enzymes of sea cucumber Apostichopus japonicus in two habitats[J]. 海洋湖沼学报（英文）, 2018,36(3):990-1001.

Qi WANG, Xiumei ZHANG, Muyan CHEN, et al. Comparison of intestinal microbiota and activities of digestive and immune-related enzymes of sea cucumber Apostichopus japonicus in two habitats[J]. Chinese Journal of Oceanology and Limnology, 2018, 36(3): 990-1001.
王琦, 张秀梅, 陈慕雁, 等. Comparison of intestinal microbiota and activities of digestive and immune-related enzymes of sea cucumber Apostichopus japonicus in two habitats[J]. 海洋湖沼学报（英文）, 2018,36(3):990-1001. DOI： 10.1007/s00343-018-7075-z.

Qi WANG, Xiumei ZHANG, Muyan CHEN, et al. Comparison of intestinal microbiota and activities of digestive and immune-related enzymes of sea cucumber Apostichopus japonicus in two habitats[J]. Chinese Journal of Oceanology and Limnology, 2018, 36(3): 990-1001. DOI： 10.1007/s00343-018-7075-z.

摘要

Abstract

Sea cucumber

Apostichopus japonicus

stock enhancement by releasing hatchery-produced seeds is a management tool used to recover its population under natural environmental conditions. To assess the suitability of releasing sites

we examined the microbiota of the gut contents of

A. japonicus

from two populations (one in sandy-muddy seagrass beds and one in rocky intertidal reefs) and the microbiota in their surrounding sediments. The activities of digestive and immune-related enzymes in the

A. japonicus

were also examined. The results indicated that higher bacterial richness and Shannon diversity index were observed in all the seagrass-bed samples. There were significant differences in intestinal and sediment microorganisms between the two habitats

with a 2.87 times higher abundance of Firmicutes in the seagrass bed sediments than that in the reefs. Meanwhile

Bacteroidetes and Actinobacteria were significantly higher abundant in the gut content of

A. japonicus

from seagrass bed than those from the reefs. In addition

the seagrass-bed samples exhibited a relatively higher abundance of potential probiotics. Principal coordinates analysis and heatmap showed the bacterial communities were classified into two groups corresponding to the two habitat types. Moreover

compared to

A. japonicus

obtained from rocky intertidal habitat

those obtained from the seagrass bed showed higher lysozyme

superoxide dismutase and protease activities. Our results suggest that bacterial communities present in seagrass beds might enhance the digestive function and immunity of

A. japonicus

. Therefore

compared with the rocky intertidal reef

seagrass bed seems to be more beneficial for the survival of

A. japonicus

关键词

Keywords

references

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Comparison of intestinal microbiota and activities of digestive and immune-related enzymes of sea cucumber Apostichopus japonicus in two habitats

DOI：10.1007/s00343-018-7075-z

摘要

Abstract

关键词

Keywords

references