Expression and characterization of a bifunctional alginate lyase named Al163 from the Antarctic bacterium <italic style="font-style: italic">Pseudoalteromonas</italic> sp. NJ-21

谢买胜; 李江; 何培青; 林学政

doi:10.1007/s00343-018-7074-0

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Expression and characterization of a bifunctional alginate lyase named Al163 from the Antarctic bacterium Pseudoalteromonas sp. NJ-21

Biology | Updated：2023-05-19

- Expression and characterization of a bifunctional alginate lyase named Al163 from the Antarctic bacterium Pseudoalteromonas sp. NJ-21
- Expression and characterization of a bifunctional alginate lyase named Al163 from the Antarctic bacterium Pseudoalteromonas sp. NJ-21
- 海洋湖沼学报（英文） 2018年36卷第4期页码：1304-1314
- Affiliations：
  
  1.Key Laboratory of Marine Bioactive Substances, The First Institute of Oceanography, State Oceanic Administration(SOA), Qingdao 266061, China
  2.Key Laboratory of Marine Natural Products of Qingdao, Qingdao 266061, China
- Author bio：
  
  LI Jiang, lijiang@fio.org.cn
- Funds：
  
  the Public Science and Technology Research Funds Project of Ocean(201505026-4);the Chinese Polar Environment Comprehensive Investigation & Assessment Programs(CHINARE2016-01-05);the Basic Scientific Research Funds of First Institute of Oceanography, State Oceanic Administration (SOA)(2014T09);the Qingdao Applied Basic Research Project(14-2-4-14-jch)
- DOI：10.1007/s00343-018-7074-0
  中图分类号：
- 收稿：2017-03-15，
  
  录用：2017-4-28，
  
  网络首发：2018-06-27，
  
  纸质出版：2018-07
- Accepted：
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谢买胜, 李江, 何培青, 等. Expression and characterization of a bifunctional alginate lyase named Al163 from the Antarctic bacterium Pseudoalteromonas sp. NJ-21[J]. 海洋湖沼学报（英文）, 2018,36(4):1304-1314.

Maisheng XIE, Jiang LI, Peiqing HE, et al. Expression and characterization of a bifunctional alginate lyase named Al163 from the Antarctic bacterium Pseudoalteromonas sp. NJ-21[J]. Chinese Journal of Oceanology and Limnology, 2018, 36(4): 1304-1314.
谢买胜, 李江, 何培青, 等. Expression and characterization of a bifunctional alginate lyase named Al163 from the Antarctic bacterium Pseudoalteromonas sp. NJ-21[J]. 海洋湖沼学报（英文）, 2018,36(4):1304-1314. DOI： 10.1007/s00343-018-7074-0.

Maisheng XIE, Jiang LI, Peiqing HE, et al. Expression and characterization of a bifunctional alginate lyase named Al163 from the Antarctic bacterium Pseudoalteromonas sp. NJ-21[J]. Chinese Journal of Oceanology and Limnology, 2018, 36(4): 1304-1314. DOI： 10.1007/s00343-018-7074-0.

摘要

Abstract

In this study

an endolytic alginate lyase

named Al163

was identified

cloned

and characterized from the Antarctic bacterium

Pseudoalteromonas

sp. NJ-21. Comparative sequence analysis showed that the predicted amino acid sequence encoded by al163 belongs to the polysaccharide lyase 6 (PL-6) family and has a molecular mass of about 80 kDa. Recombinant enzyme was purified by Ni-Sepharose affinity chromatography. Recombinant Al163 exhibited maximum activity (258 U/mg) at pH 7.0 and 40℃

and thermal stability assays showed retention of almost 90% activity after incubation at 30℃for 30 min. Al163 activity was stimulated by Cd

and Mn

but inhibited by Cu

and Ni

. Thin-layer chromatographic analysis indicated that Al163 degraded sodium alginate

polyM

and polyG

generating disaccharides and trisaccharides as the final products. Only a few bacterial strains that produce a bifunctional alginate lyase have been reported. Our results indicate that recombinant Al163 exhibits broad substrate specificity and its products exhibit low degrees of polymerization. Both properties imply high potential for use of the enzyme in several industrial fields

including cosmetics and pharmaceuticals

based on the high demand for biologically active oligosaccharides.

关键词

Keywords

references

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Expression and characterization of a bifunctional alginate lyase named Al163 from the Antarctic bacterium Pseudoalteromonas sp. NJ-21

Expression and characterization of a bifunctional alginate lyase named Al163 from the Antarctic bacterium Pseudoalteromonas sp. NJ-21

DOI：10.1007/s00343-018-7074-0

摘要

Abstract

关键词

Keywords

references