Characterization and differential expression of three GnRH forms during reproductive development in cultured turbot <italic style="font-style: italic">Schophthalmus maximus</italic>

赵春彦; 徐世宏; 丰程程; 柳意樊; 杨阳; 王彦丰; 肖永双; 宋宗诚; 刘清华; 李军

doi:10.1007/s00343-018-7068-y

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Characterization and differential expression of three GnRH forms during reproductive development in cultured turbot Schophthalmus maximus

Biology | Updated：2023-05-19

- Characterization and differential expression of three GnRH forms during reproductive development in cultured turbot Schophthalmus maximus
- Characterization and differential expression of three GnRH forms during reproductive development in cultured turbot Schophthalmus maximus
- 海洋湖沼学报（英文） 2018年36卷第4期页码：1360-1373
- Affiliations：
  
  1.Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China
  2.University of Chinese Academy of Sciences, Beijing 100049, China
  3.Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266237, China
  4.Weihai Shenghang Aquatic Product Science and Technology Co. Ltd., Weihai 264200, China
- Author bio：
  
  LIU Qinghua, qinghualiu@qdio.ac.cn
  LI Jun, junli@qdio.ac.cn
- Funds：
  
  the National Natural Science Foundation of China(31472264);the National Natural Science Foundation of China(31572602);the China Agriculture Research System(CARS-47);the Aoshan Technology Innovation Program of Qingdao National Laboratory for Marine Science and Technology(2015ASKJ02);the Scientific and Technological Innovation Project Financially Supported by Qingdao National Laboratory for Marine Science and Technology(2015ASKJ02-03-03);the Youth Innovation Promotion Association CAS and Chinese Academy of Science and Technology Service Network Planning(KFJ-EWSTS-060)
- DOI：10.1007/s00343-018-7068-y
  中图分类号：
- 收稿：2017-03-06，
  
  录用：2017-5-7，
  
  网络首发：2017-06-06，
  
  纸质出版：2018-07
- Accepted：
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赵春彦, 徐世宏, 丰程程, 等. Characterization and differential expression of three GnRH forms during reproductive development in cultured turbot Schophthalmus maximus[J]. 海洋湖沼学报（英文）, 2018,36(4):1360-1373.

Chunyan ZHAO, Shihong XU, Chengcheng FENG, et al. Characterization and differential expression of three GnRH forms during reproductive development in cultured turbot Schophthalmus maximus[J]. Chinese Journal of Oceanology and Limnology, 2018, 36(4): 1360-1373.
赵春彦, 徐世宏, 丰程程, 等. Characterization and differential expression of three GnRH forms during reproductive development in cultured turbot Schophthalmus maximus[J]. 海洋湖沼学报（英文）, 2018,36(4):1360-1373. DOI： 10.1007/s00343-018-7068-y.

Chunyan ZHAO, Shihong XU, Chengcheng FENG, et al. Characterization and differential expression of three GnRH forms during reproductive development in cultured turbot Schophthalmus maximus[J]. Chinese Journal of Oceanology and Limnology, 2018, 36(4): 1360-1373. DOI： 10.1007/s00343-018-7068-y.

摘要

Abstract

Turbots (

Schophthalmus maximus

)

one of the most important economic marine flatfish species

fail to undergo final spawning and spermiation naturally under artificial farming conditions. In vertebrates

reproduction is regulated by the brain-pituitary-gonadal axis (BPG-axis)

and gonadotropin releasing hormone (GnRH) is one of its key components. Therefore

to better understand the physiology of reproduction in the turbot

three of the genes encoding GnRH subtypes-

sbGnRH

cGnRH-Ⅱ

and

sGnRH

-were cloned and sequenced by isolating the cDNA sequences. The localizations and patterns of expression of their mRNAs were also evaluated during seasonal gonadal development. All three mRNAs were expressed abundantly in the brain;

sbGnRH

and

sGnRH

mRNAs were also detected in the gonads and pituitary gland

and

sbGnRH

expression was much higher than that of

sGnRH

indicating the critical role of sbGnRH in regulating the BPG-axis. Moreover

the brain expression patterns of

sbGnRH

and

sGnRH

mRNAs showed an increased trend during gonadal development

peaking in mature stages. This indicated the direct regulation of gonadal development by the GnRH system. In addition

cGnRH-Ⅱ

mRNA expression showed no significant variations

suggesting that cGnRH-Ⅱ is not critically involved in the control of reproduction. Further

the mRNA abundances of the three GnRH forms in the breeding season were significantly higher than those in immature and post-breeding stages in all analyzed brain areas. Therefore

we propose that sbGnRH is the most important hormone for the regulation of reproduction in turbot via the BPG-axis. These results will help in better understanding the reproductive endocrine mechanisms of turbots and lay the groundwork for additional studies aimed at comparing the reproductive physiology of wild individuals with those raised under artificial conditions.

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Keywords

references

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Characterization and differential expression of three GnRH forms during reproductive development in cultured turbot Schophthalmus maximus

Characterization and differential expression of three GnRH forms during reproductive development in cultured turbot Schophthalmus maximus

DOI：10.1007/s00343-018-7068-y

摘要

Abstract

关键词

Keywords

references