Effects of probiotic on microfloral structure of live feed used in larval breeding of turbot <italic style="font-style: italic">Scophthalmus maximus</italic>

姜燕; 张正; 王印庚; 景亚运; 廖梅杰; 荣小军; 李彬; 陈贵平; 张和森

doi:10.1007/s00343-018-7049-1

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Effects of probiotic on microfloral structure of live feed used in larval breeding of turbot Scophthalmus maximus

Aquaculture and Fisheries | Updated：2023-05-19

- Effects of probiotic on microfloral structure of live feed used in larval breeding of turbot Scophthalmus maximus
- Effects of probiotic on microfloral structure of live feed used in larval breeding of turbot Scophthalmus maximus
- 海洋湖沼学报（英文） 2018年36卷第3期页码：1002-1012
- Affiliations：
  
  1.Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071, China
  2.Qingdao General Aquatic Co. Ltd., Qingdao 266000, China
- Author bio：
  
  WANG Yingeng, wangyg@ysfri.ac.cn
- Funds：
  
  the National Natural Science Foundation of China(31302206);the Postdoctoral Applied Research Project of Qingdao(Q51201607)
- DOI：10.1007/s00343-018-7049-1
  中图分类号：
- 收稿：2017-02-22，
  
  录用：2017-3-10，
  
  网络首发：2017-04-20，
  
  纸质出版：2018-05
- Accepted：
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姜燕, 张正, 王印庚, 等. Effects of probiotic on microfloral structure of live feed used in larval breeding of turbot Scophthalmus maximus[J]. 海洋湖沼学报（英文）, 2018,36(3):1002-1012.

Yan JIANG, Zheng ZHANG, Yingeng WANG, et al. Effects of probiotic on microfloral structure of live feed used in larval breeding of turbot Scophthalmus maximus[J]. Chinese Journal of Oceanology and Limnology, 2018, 36(3): 1002-1012.
姜燕, 张正, 王印庚, 等. Effects of probiotic on microfloral structure of live feed used in larval breeding of turbot Scophthalmus maximus[J]. 海洋湖沼学报（英文）, 2018,36(3):1002-1012. DOI： 10.1007/s00343-018-7049-1.

Yan JIANG, Zheng ZHANG, Yingeng WANG, et al. Effects of probiotic on microfloral structure of live feed used in larval breeding of turbot Scophthalmus maximus[J]. Chinese Journal of Oceanology and Limnology, 2018, 36(3): 1002-1012. DOI： 10.1007/s00343-018-7049-1.

摘要

Abstract

The effects of an exogenous probiotic (

Bacillus amyloliquefaciens

) on microbial community structure of

Branchionus plicatils

and

Artemia sinica

were evaluated in this study during turbot (

Scophthalmus maximus

) larval breeding. The analysis and comparison of the microfloral composition of live feed with probiotic was conducted using the Illumina HiSeq PE250. The abundance of microbial species and diversity of microflora in live feed with

B. amyloliquefaciens

were higher than those in the control. The microfloral composition was similar among the three replicate experimental groups of

B. plicatils

compared with the control after enrichment.

Lactococcus

Pseudoalteromonas

and

Alteromonas

were always dominant. Additionally

some other bacterial species became dominant during the enrichment process. The microbial community during nutrient enrichment of

A. sinica

was rather similar among the three control replicates. Relative abundance of

Cobetia

sp.

the most dominant species

was 54%-65.2%. Similarity in the microbial community was still high after adding

B. amyloliquefaciens

. Furthermore

Pseudoalteromonas

and

Alteromonas

replaced

Cobetia

as the dominant species

and the abundance of

Cobetia

decreased to 4.3%-25.3%. Mean common ratios at the operational taxonomic unit level were 50%-60% between the two

B. plicatils

and

A. sinica

treatments. Therefore

the microbial community structure changed after adding

B. amyloliquefaciens

during nutrient enrichment of

B. plicatils

A. sinica

and tended to stabilize. Additionally

the abundance of

Vibrio

in any kind of live feed was not significantly different from that in the control. These results will help improve the microflora of

B. plicatils

and

A. sinica

and can be used to understand the multiple-level transfer role of probiotic species among probiotic products

microflora of live feed

and fish larvae.

关键词

Keywords

references

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Effects of probiotic on microfloral structure of live feed used in larval breeding of turbot Scophthalmus maximus

Effects of probiotic on microfloral structure of live feed used in larval breeding of turbot Scophthalmus maximus

DOI：10.1007/s00343-018-7049-1

摘要

Abstract

关键词

Keywords

references