Denitrification potential evaluation of a newly indigenous aerobic denitrifier isolated from largemouth bass <italic style="font-style: italic">Micropterus salmoides</italic> culture pond

王翠翠; 张凯; 谢骏; 刘其根; 余德光; 王广军; 郁二蒙; 龚望宝; 李志斐

doi:10.1007/s00343-018-7029-5

Your Location：

Home >

Browse articles >

Denitrification potential evaluation of a newly indigenous aerobic denitrifier isolated from largemouth bass Micropterus salmoides culture pond

Biology | Updated：2023-05-19

- Denitrification potential evaluation of a newly indigenous aerobic denitrifier isolated from largemouth bass Micropterus salmoides culture pond
- Denitrification potential evaluation of a newly indigenous aerobic denitrifier isolated from largemouth bass Micropterus salmoides culture pond
- 海洋湖沼学报（英文） 2018年36卷第3期页码：913-925
- Affiliations：
  
  1.Key Laboratory of Tropical and Subtropical Fishery Resource Application and Cultivation, Ministry of Agriculture, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510380, China
  2.College of Fisheries and Life Sciences, Shanghai Ocean University, Shanghai 201306, China
- Author bio：
  
  XIE Jun, xiejunhy01@126.com
- Funds：
  
  the National Key Technology R&D Program of China(2012BAD25B04);the National Key Technology R&D Program of China(2012BAD25B01);the Earmarked Fund for Modern Agro-Industry Technology Research Systemv(CARS-46-17);the National Natural Science Foundation of China(31302201);the Pearl River S&T Nova Program of Guangzhou(2014J2200088);the Science and Technology Program of Guangzhou(201707010311)
- DOI：10.1007/s00343-018-7029-5
  中图分类号：
- 收稿：2017-03-30，
  
  录用：2017-5-4，
  
  网络首发：2017-05-27，
  
  纸质出版：2018-05
- Accepted：
Scan QR Code
王翠翠, 张凯, 谢骏, 等. Denitrification potential evaluation of a newly indigenous aerobic denitrifier isolated from largemouth bass Micropterus salmoides culture pond[J]. 海洋湖沼学报（英文）, 2018,36(3):913-925.

Cuicui WANG, Kai ZHANG, Jun XIE, et al. Denitrification potential evaluation of a newly indigenous aerobic denitrifier isolated from largemouth bass Micropterus salmoides culture pond[J]. Chinese Journal of Oceanology and Limnology, 2018, 36(3): 913-925.
王翠翠, 张凯, 谢骏, 等. Denitrification potential evaluation of a newly indigenous aerobic denitrifier isolated from largemouth bass Micropterus salmoides culture pond[J]. 海洋湖沼学报（英文）, 2018,36(3):913-925. DOI： 10.1007/s00343-018-7029-5.

Cuicui WANG, Kai ZHANG, Jun XIE, et al. Denitrification potential evaluation of a newly indigenous aerobic denitrifier isolated from largemouth bass Micropterus salmoides culture pond[J]. Chinese Journal of Oceanology and Limnology, 2018, 36(3): 913-925. DOI： 10.1007/s00343-018-7029-5.

摘要

Abstract

This work evaluates the application potential of a new indigenous aerobic denitrifier

strain

Pseudomonas

CW-2

isolated from a largemouth bass culture pond. The rate of ammonium-N removal by strain CW-2 was approximately 97% at a DO concentration of 5.2 mg/L. Furthermore

when nitrate and ammonia coexisted

the strain gave priority to assimilating ammonia

and thereafter to denitrification. Under optimal cultivation conditions

citrate and acetate were the carbon resources

C/N was 8

dissolved oxygen was 5.2 mg/L

and pH was 7; the removal rate of ammonium reached nearly 90%. The changing patterns of different bacteria in strain CW-2-treated and the control pond water were also compared. Lower levels of ammonia

nitrite

and phosphates were observed in the treated water as compared with the controls. Meanwhile

phylum-level distributions of the bacterial OTUs revealed that

Proteobacteria

Bacteroidetes

Planctomycetes

and

Nitrospirae

continuously changed their relative abundances in relation to carbon and the addition of strain CW-2; this finding implies that the conventional denitrification process was weakened under the effects of carbon or the presence of strain CW-2. We propose that strain CW-2 is a promising organism for the removal of ammonium in intensive fish culture systems

according to our evaluations of its denitrification performance.

关键词

Keywords

references

Abufayed A A, Schroeder E D. 1986. Kinetics and stoichiometry of SBR/denitrification with a primary sludge carbon source. J. Water Pollut. Control Fed., 58:398-405.

Carpenter J H. 1965. The Chesapeake Bay institute technique for the Winkler dissolved oxygen method. Limnology and Oceanography, 10(1):141-143.

Chen P Z, Li J, Li Q X, Wang Y C, Li S P, Ren T Z, Wang L G. 2012. Simultaneous heterotrophic nitrification and aerobic denitrification by bacterium Rhodococcus sp. CPZ24.Bioresource Technology, 116:266-270.

Garbeva P, Baggs E M, Prosser J I. 2007. Phylogeny of nitrite reductase ( nirK ) and nitric oxide reductase ( norB ) genes from Nitrosospira species isolated from soil. FEMS Microbiology Letters, 266(1):83-89.

GBT 7493-1987 Water quality-determination of nitrogen(nitrite)-spectrophotometric method. 1987. The State Environmental Protection Administration of China, p. 144-148.

Grguric G, Wetmore S S, Fournier R W. 2000. Biological denitrification in a closed seawater system. Chemosphere, 40 (5):549-555.

Guo L Y, Chen Q K, Fang F, Hu Z X, Wu J, Miao A J, Xiao L, Chen X F, Yang L Y. 2013. Application potential of a newly isolated indigenous aerobic denitrifier for nitrate and ammonium removal of eutrophic lake water.Bioresource Technology, 142:45-51.

Huang H K, Tseng S K. 2001. Nitrate reduction by Citrobacter diversusunder aerobic environment.AppliedMicrobiology & Biotechnology, 55 (1):90-94.

Joo H S, Hirai M, Shoda M. 2005. Characteristics of ammonium removal by heterotrophic nitrification-aerobic denitrification by Alcaligenes faecalis No. 4. Journal of Bioscience and Bioengineering, 100(2):184-191.

Kim M, Jeong S Y, Yoon S J, Cho S J, Kim Y H, Kim M J, Ryu E Y, Lee S J. 2008. Aerobic denitrification of Pseudomonas putida AD-21 at different C/N ratios. Journal of Bioscience and Bioengineering, 106(5):498-502.

Kolndadacha O D, Adikwu I A, Okaeme A N, Atiribom R Y, Mohammed A, Musa Y M. 2011. The role of probiotics in aquaculture in Nigeria-a review. Continental Journal of Fisheries and Aquatic Sciences, 5(1):8-15.

Kumar G S, Ramakrishnan A, Hung Y T. 2010. Simultaneous removal of carbon and nitrogen from domestic wastewater in an aerobic RBC. In : Wang L K, Tay J H, Tay S T L, Hung Y T eds. Environmental Bioengineering: Volume 11. Humana Press, Totowa, NJ, USA. p. 403-443.

Liang Y H, Li D, Zhang X J, Zeng H P, Yang Z, Zhang J. 2014.Microbial characteristics and nitrogen removal of simultaneous partial nitrification, anammox and denitrification (SNAD) process treating low C/N ratio sewage. Bioresource Technology, 169:103-109.

Lukow T, Diekmann H. 1997. Aerobic denitrification by a newly isolated heterotrophic bacterium strain TLl.Biotechnology Letters, 19(11):1 157-1 159.

Michael E T, Amos S O, Hussaini L T. 2014. A review on probiotics application in aquaculture. Fisheries and Aquaculture Journal, 5(4):111.

Miyahara M, Kim S W, Fushinobu S, Takaki K, Yamada T, Watanabe A, Miyauchi K, Endo G, Wakagi T, Shoun H. 2010. Potential of aerobic denitrification by Pseudomonas stutzeri TR2 to reduce nitrous oxide emissions from wastewater treatment plants. Applied and Environmental Microbiology, 76(14):4 619-4 625.

Obaja D, Macé S, Mata-Alvarez J. 2005. Biological nutrient removal by a sequencing batch reactor (SBR) using an internal organic carbon source in digested piggery wastewater. Bioresource Technology, 96(1):7-14.

Pai S L, Chong N M, Chen C H. 1999. Potential applications of aerobic denitrifying bacteria as bioagents in wastewater treatment. Bioresource Technology, 68(2):179-185.

Patureau D, Bernet N, Delgenès J P, Moletta R. 2000. Effect of dissolved oxygen and carbon-nitrogen loads on denitrification by an aerobic consortium. Applied Microbiology and Biotechnology, 54(4):535-542.

Qiu X F, Wang T W, Zhong X M, Du G C, Chen J. 2012.Screening and characterization of an aerobic nitrifying-denitrifying bacterium from activated sludge.Biotechnology and Bioprocess Engineering, 17(2):353-360.

Robertson L A, van Niel E W J, Torremans R A M, Kuenen J G. 1988. Simultaneous nitrification and denitrification in aerobic chemostat cultures of Thiosphaera pantotropha .Applied and Environmental Microbiology, 54(11):2 812-2 818.

Robertson L A, Kuenen J G. 1984. Aerobic denitrification:a controversy revived. Archives of Microbiology, 139(4):351-354.

Shao Q, Yu X B. 2008. Isolation and characterization of a strain denitrobacteria. Biotechnology, 18(3):63-65. (in Chinese with English abstract)

Šiljeg M, Foglar L, Kukučka M. 2010. The ground water ammonium sorption onto Croatian and Serbian clinoptilolite. Journal of Hazardous Materials, 178(1-3):572-577.

Skjermo J, Bakke I, Dahle S W, Vadstein O. 2015. Probiotic strains introduced through live feed and rearing water have low colonizing success in developing Atlantic cod larvae. Aquaculture, 438:17-23.

Song Z F, An J, Fu G H, Yang X L. 2011. Isolation and characterization of an aerobic denitrifying Bacillus sp.YX-6 from shrimp culture ponds. Aquaculture, 319(1-2):188-193.

Su J J, Yeh K S, Tseng P W. 2006. A strain of Pseudomonas sp. isolated from piggery wastewater treatment systems with heterotrophic nitrification capability in Taiwan. Current Microbiology, 53(1):77-81.

Takaya N, Catalan-Sakairi M A B, Sakaguchi Y, Kato I, Zhou Z M, Shoun H. 2003. Aerobic denitrifying bacteria that produce low levels of nitrous oxide. Applied and Environmental Microbiology, 69(6):3 152-3 157.

Tang J Y, Dai Y X, Li Y M, Qin J G, Wang Y. 2016. Can application of commercial microbial products improve fish growth and water quality in freshwater polyculture?North American Journal of Aquaculture, 78(2):154-160.

Taylor S M, He Y L, Zhao B, Huang J. 2009. Heterotrophic ammonium removal characteristics of an aerobic heterotrophic nitrifying-denitrifying bacterium, Providencia rettgeri YL. Journal of Environmental Sciences, 21(10):1 336-1 341.

Throbäck I N, Enwall K, Jarvis A, Hallin S. 2004. Reassessing PCR primers targeting nirS , nirK and nosZ genes for community surveys of denitrifying bacteria with DGGE.FEMS Microbiology Ecology, 49(3):401-417.

van Niel E W J, Braber K J, Robertson L A, Kuene J G. 1992.Heterotrophic nitrification and aerobic denitrification in Alcaligenes faecalis strain TUD. Antonie van Leeuwenhoek, 62(3):231-237.

Verschuere L, Rombaut G, Sorgeloos P, Verstraete W. 2000.Probiotic bacteria as biological control agents in aquaculture. Microbiology and Molecular Biology Reviews, 64(4):655-671.

Wan C L, Yang X, Lee D J, Du M A, Wan F, Chen C. 2011.Aerobic denitrification by novel isolated strain using NO 2 - -N as nitrogen source. Bioresource Technology, 102(15):7 244-7 248.

Wang P, Yuan Y Z, Li Q, Yang J Z, Zheng Y L, He M Q, Geng H, Xiong L, Liu D L. 2013. Isolation and immobilization of new aerobic denitrifying bacteria. International Biodeterioration & Biodegradation, 76:12-17.

Xiang M F, Wang P, Liu X T, Zhai Q. 2006. Selection and identification of aerobic denitrifiers in denitrification process of wastewater. Food Science and Technology, 31(7):153-156. (in Chinese with English abstract)

Xu Y T. 1994. The effect of different carbon sources on biological denitrification. Chinese Journal of Environmental Science, 15(2):28-32, 44. (in Chinese with English abstract)

Xu Z N, Lin X T, Lin Q, Yang Y F, Wang Y X. 2007. Nitrogen, phosphorus, and energy waste outputs of four marine cage-cultured fish fed with trash fish. Aquaculture, 263(1-4):130-141.

Yao M J, Rui J P, Li J B, Dai Y M, Bai Y F, Heděnec P, Wang J M, Zhang S H, Pei K Q, Liu C, Wang Y F, He Z L, Frouz J, Li X Z. 2014. Rate-specific responses of prokaryotic diversity and structure to nitrogen deposition in the Leymus chinensis steppe. Soil Biology and Biochemistry, 79:81-90.

Ye L, Shao M F, Zhang T, Tong A H Y, Lok S. 2011. Analysis of the bacterial community in a laboratory-scale nitrification reactor and a wastewater treatment plant by 454-pyrosequencing. Water Research, 45(15):4 390-4 398.

Ye L, Zhang T. 2010. Estimation of nitrifier abundances in a partial nitrification reactor treating ammonium-rich saline wastewater using DGGE, T-RFLP and mathematical modeling. Applied Microbiology and Biotechnology, 88(6):1 403-1 412.

Zhang J B, Wu P X, Hao B, Yu Z N. 2011. Heterotrophic nitrification and aerobic denitrification by the bacterium Pseudomonas stutzeri YZN-001. Bioresource Technology, 102(21):9 866-9 869.

Zhang Q L, Liu Y, Ai G M, Miao L L, Zheng H Y, Liu Z P. 2012. The characteristics of a novel heterotrophic nitrification-aerobic denitrification bacterium, Bacillus methylotrophicus strain L7. Bioresource Technology, 108:35-44.

Zhao Y G, Fang Y, Jin Y L, Huang J, Ma X R, He K Z, He Z M, Wang F, Zhao H. 2015. Microbial community and removal of nitrogen via the addition of a carrier in a pilotscale duckweed-based wastewater treatment system.Bioresource Technology, 179:549-558.

Zheng H Y, Liu Y, Gao X Y, Ai G M, Miao L L, Liu Z P. 2012.Characterization of a marine origin aerobic nitrifyingdenitrifying bacterium. Journal of Bioscience and Bioengineering, 114(1):33-37.

Zhu L, Ding W, Feng L J, Dai X, Xu X Y. 2012a. Characteristics of an aerobic denitrifier that utilizes ammonium and nitrate simultaneously under the oligotrophic niche.Environmental Science and Pollution Research, 19(8):3 185-3 191.

Zhu L, Ding W, Feng L J, Kong Y, Xu J, Xu X Y. 2012b.Isolation of aerobic denitrifiers and characterization for their potential application in the bioremediation of oligotrophic ecosystem. Bioresource Technology, 108:1-7.

浏览量

Downloads

CSCD

文章被引用时，请邮件提醒。

Submit

工具集

关联资源

Protective efficacy evaluation of immunogenic protein AHA_3793 of Aeromonas hydrophila as vaccine candidate for largemouth bass Micropterus salmoides

水下超声速气流流场非定常特性研究

高压涡轮封严流与主流相互作用的机理研究

导流锥结构参数对燃气弹射筒内压力冲击平滑效果影响研究

双转子轴间滑油颗粒运动轨迹参数化模型

Denitrification potential evaluation of a newly indigenous aerobic denitrifier isolated from largemouth bass Micropterus salmoides culture pond

Denitrification potential evaluation of a newly indigenous aerobic denitrifier isolated from largemouth bass Micropterus salmoides culture pond

DOI：10.1007/s00343-018-7029-5

摘要

Abstract

关键词

Keywords

references