Stocking density affects the growth performance and metabolism of Amur sturgeon by regulating expression of genes in the GH/IGF axis

任源远; 温海深; 李昀; 李吉方

doi:10.1007/s00343-018-7018-8

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Stocking density affects the growth performance and metabolism of Amur sturgeon by regulating expression of genes in the GH/IGF axis

Aquaculture and Fisheries | Updated：2023-05-19

- Stocking density affects the growth performance and metabolism of Amur sturgeon by regulating expression of genes in the GH/IGF axis
- Stocking density affects the growth performance and metabolism of Amur sturgeon by regulating expression of genes in the GH/IGF axis
- 海洋湖沼学报（英文） 2018年36卷第3期页码：956-972
- Affiliations：
  
  Key Laboratory of Mariculture(Ocean University of China), Ministry of Education, Qingdao 266003, China
- Author bio：
  
  WEN Haishen, wenhaishen@ouc.edu.cn
- Funds：
  
  the National Special Research Fund for Non-Profit Sector (Agriculture)(201003055)
- DOI：10.1007/s00343-018-7018-8
  中图分类号：
- 收稿：2017-01-16，
  
  录用：2017-3-9，
  
  网络首发：2017-03-13，
  
  纸质出版：2018-05
- Accepted：
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任源远, 温海深, 李昀, 等. Stocking density affects the growth performance and metabolism of Amur sturgeon by regulating expression of genes in the GH/IGF axis[J]. 海洋湖沼学报（英文）, 2018,36(3):956-972.

Yuanyuan REN, Haishen WEN, Yun LI, et al. Stocking density affects the growth performance and metabolism of Amur sturgeon by regulating expression of genes in the GH/IGF axis[J]. Chinese Journal of Oceanology and Limnology, 2018, 36(3): 956-972.
任源远, 温海深, 李昀, 等. Stocking density affects the growth performance and metabolism of Amur sturgeon by regulating expression of genes in the GH/IGF axis[J]. 海洋湖沼学报（英文）, 2018,36(3):956-972. DOI： 10.1007/s00343-018-7018-8.

Yuanyuan REN, Haishen WEN, Yun LI, et al. Stocking density affects the growth performance and metabolism of Amur sturgeon by regulating expression of genes in the GH/IGF axis[J]. Chinese Journal of Oceanology and Limnology, 2018, 36(3): 956-972. DOI： 10.1007/s00343-018-7018-8.

摘要

Abstract

The effects of stocking density on the growth and metabolism of Amur sturgeon were assessed. Amur sturgeon were grown for 70 days at three different stocking densities (low stocking density

LSD:5.5 kg/m

; medium stocking density

MSD:8.0 kg/m

; and high stocking density

HSD:11.0 kg/m

)

and the biometric index

muscle composition

and serum biochemical parameters were evaluated. In addition

pituitary

liver

and muscle samples were collected for gene cloning and expression analyses. After 70 days of growth

the fish maintained at HSD had significantly lower final body weight and specific growth rate

and a higher feed conversion ratio than those of the fish in the MSD and LSD groups. The HSD group had the lowest lipid and protein concentrations in serum and muscle. The serum cortisol concentration increased significantly in the HSD group

indicating that the stress-response system was activated in these fish. There was no change in the concentration of serum insulin-like growth factor 2 (IGF-2)

while the concentrations of serum growth hormone (GH) and insulin-like growth factor 1 (IGF-1) decreased in the HSD group. The full-length cDNAs of

and

IGF-2

genes (995-bp and 1 207-bp long

respectively)

were cloned and analyzed. In the HSD group

the expressions of

in the pituitary and growth hormone receptor (

GHR

) and

IGF-1

in the liver were down-regulated at the end of the 70-day experiment. In the HSD group

the transcript level of

IGF-2

significantly decreased in the liver

but did not change in muscle. Overall

our results indicated that a HSD negatively affects the growth performance and leads to changes in lipid and protein metabolism in Amur sturgeon. The down-regulated expression of genes related to the GH/IGF axis may be responsible for the poor growth performance of Amur sturgeon under crowding stress.

关键词

Keywords

references

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