Transcriptome profiling analysis of <italic style="font-style: italic">Mactra veneriformis</italic> by deep sequencing after exposure to 2, 2', 4, 4'-tetrabromodiphenyl ether

鹏举 石; 世航 董; 焕君 张; 培亮 王; 壮 牛; 燕 房

doi:10.1007/s00343-018-6347-y

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Transcriptome profiling analysis of Mactra veneriformis by deep sequencing after exposure to 2, 2', 4, 4'-tetrabromodiphenyl ether

Biology | Updated：2023-05-19

- Transcriptome profiling analysis of Mactra veneriformis by deep sequencing after exposure to 2, 2', 4, 4'-tetrabromodiphenyl ether
- Transcriptome profiling analysis of Mactra veneriformis by deep sequencing after exposure to 2, 2', 4, 4'-tetrabromodiphenyl ether
- 海洋湖沼学报（英文） 2018年36卷第2期页码：490-507
- Affiliations：
  
  1.School of Agriculture, Ludong University, Yantai 264025, China
  2.Shandong Marine Resource and Environment Research Institute, Shandong Provincial Key Laboratory of Restoration for Marine Ecology, Yantai 264006, China
  3.Weihai Hongrun Marine Technology Co. Ltd., Weihai 264200, China
- Author bio：
  
  FANG Yan, fly12689@163.com
- Funds：
  
  the National Natural Science Foundation of China(41206120);the Development Plan of Science and Technology in Yantai(2011062);the National Special Research Fund for Non-Profit Marine Sector(201205023);the Ludong University Research Funding(210-32040301)
- DOI：10.1007/s00343-018-6347-y
  中图分类号：
- 收稿：2016-12-27，
  
  录用：2017-2-3，
  
  网络首发：2018-02-14，
  
  纸质出版：2018-03
- Accepted：
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鹏举石, 世航董, 焕君张, 等. Transcriptome profiling analysis of Mactra veneriformis by deep sequencing after exposure to 2, 2', 4, 4'-tetrabromodiphenyl ether[J]. 海洋湖沼学报（英文）, 2018,36(2):490-507.

SHI Pengju, DONG Shihang, ZHANG Huanjun, et al. Transcriptome profiling analysis of Mactra veneriformis by deep sequencing after exposure to 2, 2', 4, 4'-tetrabromodiphenyl ether[J]. Chinese Journal of Oceanology and Limnology, 2018, 36(2): 490-507.
鹏举石, 世航董, 焕君张, 等. Transcriptome profiling analysis of Mactra veneriformis by deep sequencing after exposure to 2, 2', 4, 4'-tetrabromodiphenyl ether[J]. 海洋湖沼学报（英文）, 2018,36(2):490-507. DOI： 10.1007/s00343-018-6347-y.

SHI Pengju, DONG Shihang, ZHANG Huanjun, et al. Transcriptome profiling analysis of Mactra veneriformis by deep sequencing after exposure to 2, 2', 4, 4'-tetrabromodiphenyl ether[J]. Chinese Journal of Oceanology and Limnology, 2018, 36(2): 490-507. DOI： 10.1007/s00343-018-6347-y.

摘要

Abstract

Polybrominated diphenyl ethers (PBDEs) are ubiquitous global pollutants

which are known to have immune

development

reproduction

and endocrine toxicity in aquatic organisms

including bivalves. 2

4'-Tetrabromodiphenyl ether (BDE-47) is the predominant PBDE congener detected in environmental samples and the tissues of organisms. However

the mechanism of its toxicity remains unclear. In this study

high-throughput sequencing was performed using the clam

Mactra veneriformis

a good model for toxicological research

to clarify the transcriptomic response to BDE-47 and the mechanism responsible for the toxicity of BDE-47. The clams were exposed to 5 μg/L BDE-47 for 3 days and the digestive glands were sampled for high-throughput sequencing analysis. We obtained 127 648

154 225

and 124 985 unigenes by de novo assembly of the control group reads (CG)

BDE-47 group reads (BDEG)

and control and BDE-47 reads (CG

BDEG)

respectively. We annotated 32 176 unigenes from the CG

BDEG reads using the NR database. We categorized 24 401 unigenes into 25 functional COG clusters and 21 749 unigenes were assigned to 259 KEGG pathways. Moreover

17 625 differentially expressed genes (DEGs) were detected

with 10 028 upregulated DEGs and 7 597 downregulated DEGs. Functional enrichment analysis showed that the DEGs were involved with detoxification

antioxidant defense

immune response

apoptosis

and other functions. The mRNA expression levels of 26 DEGs were verified by quantitative real-time PCR

which demonstrated the high agreement between the two methods. These results provide a good basis for future research using the

M. veneriformis

model into the mechanism of PBDEs toxicity and molecular biomarkers for BDE-47 pollution. The regulation and interaction of the DEGs would be studied in the future for clarifying the mechanism of PBDEs toxicity.

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Keywords

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Transcriptome profiling analysis of Mactra veneriformis by deep sequencing after exposure to 2, 2', 4, 4'-tetrabromodiphenyl ether

Transcriptome profiling analysis of Mactra veneriformis by deep sequencing after exposure to 2, 2', 4, 4'-tetrabromodiphenyl ether

DOI：10.1007/s00343-018-6347-y

摘要

Abstract

关键词

Keywords

references