Development and evaluation of specific PCR primers targeting the ribosomal DNA-internal transcribed spacer (ITS) region of peritrich ciliates in environmental samples

苏蕾; 张倩倩; 龚骏

doi:10.1007/s00343-018-6326-3

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Development and evaluation of specific PCR primers targeting the ribosomal DNA-internal transcribed spacer (ITS) region of peritrich ciliates in environmental samples

Updated：2023-05-19

- Development and evaluation of specific PCR primers targeting the ribosomal DNA-internal transcribed spacer (ITS) region of peritrich ciliates in environmental samples
- Development and evaluation of specific PCR primers targeting the ribosomal DNA-internal transcribed spacer (ITS) region of peritrich ciliates in environmental samples
- 海洋湖沼学报（英文） 2018年36卷第3期页码：818-826
- Affiliations：
  
  1.Laboratory of Microbial Ecology and Matter Cycles, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, China
  2.University of Chinese Academy of Sciences, Beijing 100049, China
- Author bio：
  
  GONG Jun, jgong@yic.ac.cn
- Funds：
  
  the National Natural Science Foundation of China(31572255);the National Natural Science Foundation of China(41522604);the National Natural Science Foundation of China(31301867);the Strategic Priority Research Program of CAS(XDA11020702);the Science and Technology Development Program of Yantai(2014ZH073)
- DOI：10.1007/s00343-018-6326-3
  中图分类号：
- 收稿：2016-12-08，
  
  录用：2017-2-3，
  
  网络首发：2017-03-28，
  
  纸质出版：2018-05
- Accepted：
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苏蕾, 张倩倩, 龚骏. Development and evaluation of specific PCR primers targeting the ribosomal DNA-internal transcribed spacer (ITS) region of peritrich ciliates in environmental samples[J]. 海洋湖沼学报（英文）, 2018,36(3):818-826.

Lei SU, Qianqian ZHANG, Jun GONG. Development and evaluation of specific PCR primers targeting the ribosomal DNA-internal transcribed spacer (ITS) region of peritrich ciliates in environmental samples[J]. Chinese Journal of Oceanology and Limnology, 2018, 36(3): 818-826.
苏蕾, 张倩倩, 龚骏. Development and evaluation of specific PCR primers targeting the ribosomal DNA-internal transcribed spacer (ITS) region of peritrich ciliates in environmental samples[J]. 海洋湖沼学报（英文）, 2018,36(3):818-826. DOI： 10.1007/s00343-018-6326-3.

Lei SU, Qianqian ZHANG, Jun GONG. Development and evaluation of specific PCR primers targeting the ribosomal DNA-internal transcribed spacer (ITS) region of peritrich ciliates in environmental samples[J]. Chinese Journal of Oceanology and Limnology, 2018, 36(3): 818-826. DOI： 10.1007/s00343-018-6326-3.

摘要

Abstract

Peritrich ciliates are highly diverse and can be important bacterial grazers in aquatic ecosystems. Morphological identifications of peritrich species and assemblages in the environment are time-consuming and expertise-demanding. In this study

two peritrich-specific PCR primers were newly designed to amplify a fragment including the internal transcribed spacer (ITS) region of ribosomal rDNA from environmental samples. The primers showed high specificity in silico

and in tests with peritrich isolates and environmental DNA. Application of these primers in clone library construction and sequencing yielded exclusively sequences of peritrichs for water and sediment samples. We also found the ITS1

ITS2

ITS

D1 region of 28S rDNA

and ITS+D1 region co-varied with

and generally more variable than

the V9 region of 18S rDNA in peritrichs. The newly designed specific primers thus provide additional tools to study the molecular diversity

community composition

and phylogeography of these ecologically important protists in different systems.

关键词

Keywords

references

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