A thermostable serralysin inhibitor from marine bacterium <italic style="font-style: italic">Flavobacterium</italic> sp. YS-80-122

梁朋娟; 李尚勇; 王昆; 王芳; 邢孟欣; 郝建华; 孙谧

doi:10.1007/s00343-018-6266-y

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A thermostable serralysin inhibitor from marine bacterium Flavobacterium sp. YS-80-122

Biology | Updated：2023-05-19

- A thermostable serralysin inhibitor from marine bacterium Flavobacterium sp. YS-80-122
- A thermostable serralysin inhibitor from marine bacterium Flavobacterium sp. YS-80-122
- 海洋湖沼学报（英文） 2018年36卷第2期页码：483-489
- Affiliations：
  
  1.Key Laboratory of Polar Fisheries Development, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China
  2.Laboratory for Marine Drugs and Bioproducts, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071, China
  3.Shanghai Ocean University, Shanghai 201306, China
- Author bio：
  
  HAO Jianhua, haojh@ysfri.ac.cn
  SUN Mi, sunmi@ysfri.ac.cn
- Funds：
  
  the National Natural Science Foundation of China(41376175);the Joint Funds of the National Natural Science Foundation of China(U1406402-5);the International S & T Cooperation Program of China(2014DFG30890);the Qingdao Science and Technology Plan Project(14-2-4-11-jch);the National Science Foundation for PostDoctoral Scientists of China(2016M590673);the Application Foundation of Qingdao for Post-Doctoral Scientists of China(Q51201601)
- DOI：10.1007/s00343-018-6266-y
  中图分类号：
- 收稿：2016-10-13，
  
  录用：2016-12-26，
  
  网络首发：2018-02-22，
  
  纸质出版：2018-03
- Accepted：
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梁朋娟, 李尚勇, 王昆, 等. A thermostable serralysin inhibitor from marine bacterium Flavobacterium sp. YS-80-122[J]. 海洋湖沼学报（英文）, 2018,36(2):483-489.

Pengjuan LIANG, Shangyong LI, Kun WANG, et al. A thermostable serralysin inhibitor from marine bacterium Flavobacterium sp. YS-80-122[J]. Chinese Journal of Oceanology and Limnology, 2018, 36(2): 483-489.
梁朋娟, 李尚勇, 王昆, 等. A thermostable serralysin inhibitor from marine bacterium Flavobacterium sp. YS-80-122[J]. 海洋湖沼学报（英文）, 2018,36(2):483-489. DOI： 10.1007/s00343-018-6266-y.

Pengjuan LIANG, Shangyong LI, Kun WANG, et al. A thermostable serralysin inhibitor from marine bacterium Flavobacterium sp. YS-80-122[J]. Chinese Journal of Oceanology and Limnology, 2018, 36(2): 483-489. DOI： 10.1007/s00343-018-6266-y.

摘要

Abstract

Serralysin inhibitors have been proposed as potent drugs against many diseases and may help to prevent further development of antibiotic-resistant pathogenic bacteria. In this study

a novel serralysin inhibitor gene

lupI

was cloned from the marine bacterium

Flavobacterium

sp. YS-80-122 and expressed in

Escherichia coli

. The deduced serralysin inhibitor

LupI

shows

40% amino acid identity to other reported serralysin inhibitors. Multiple sequence alignment and phylogenetic analysis of LupI with other serralysin inhibitors indicated that LupI was a novel type of serralysin inhibitor. The inhibitory constant for LupI towards its target metalloprotease was 0.64 μmol/L. LupI was thermostable at high temperature

in which 35.6%-90.7% of its inhibitory activity was recovered after treatment at 100℃ for 1-60 min followed by incubation at 0℃. This novel inhibitor may represent a candidate drug for the treatment of serralysin-related infections.

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Keywords

references

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A thermostable serralysin inhibitor from marine bacterium Flavobacterium sp. YS-80-122

A thermostable serralysin inhibitor from marine bacterium Flavobacterium sp. YS-80-122

DOI：10.1007/s00343-018-6266-y

摘要

Abstract

关键词

Keywords

references