Effects of dietary supplementation of <italic style="font-style: italic">Ulva pertusa</italic> and nonstarch polysaccharide enzymes on gut microbiota of <italic style="font-style: italic">Siganus canaliculatus</italic>

张新旭; 吴惠娟; 李忠贞; 李远友; 王树启; 朱大世; 温小波; 李升康

doi:10.1007/s00343-017-6235-x

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Effects of dietary supplementation of Ulva pertusa and nonstarch polysaccharide enzymes on gut microbiota of Siganus canaliculatus

Biology | Updated：2023-05-19

- Effects of dietary supplementation of Ulva pertusa and nonstarch polysaccharide enzymes on gut microbiota of Siganus canaliculatus
- Effects of dietary supplementation of Ulva pertusa and nonstarch polysaccharide enzymes on gut microbiota of Siganus canaliculatus
- 海洋湖沼学报（英文） 2018年36卷第2期页码：438-449
- Affiliations：
  
  1.Guangdong Provincial Key Laboratory of Marine Biotechnology, Shantou University, Shantou 515063, China
  2.Marine Biology Institute, Shantou University, Shantou 515063, China
- Author bio：
  
  LI Shengkang, lisk@stu.edu.cn
- Funds：
  
  the National Natural Science Foundation of China(41641053);the National Natural Science Foundation of China(41376176);the National Natural Science Foundation of China(41276179);the Guangdong Natural Science Foundation(S2011030005257);the China Postdoctoral Science Foundation(2016M602501);Supplementary material (Supplementary Fig.S1 and Tables S1–S7) is available in the online version of this article at https://doi.org/10.1007/s00343-017-6235-x.
- DOI：10.1007/s00343-017-6235-x
  中图分类号：
- 收稿：2016-09-19，
  
  录用：2016-11-10，
  
  网络首发：2016-12-26，
  
  纸质出版：2018-03
- Accepted：
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张新旭, 吴惠娟, 李忠贞, 等. Effects of dietary supplementation of Ulva pertusa and nonstarch polysaccharide enzymes on gut microbiota of Siganus canaliculatus[J]. 海洋湖沼学报（英文）, 2018,36(2):438-449.

Xinxu ZHANG, Huijuan WU, Zhongzhen LI, et al. Effects of dietary supplementation of Ulva pertusa and nonstarch polysaccharide enzymes on gut microbiota of Siganus canaliculatus[J]. Chinese Journal of Oceanology and Limnology, 2018, 36(2): 438-449.
张新旭, 吴惠娟, 李忠贞, 等. Effects of dietary supplementation of Ulva pertusa and nonstarch polysaccharide enzymes on gut microbiota of Siganus canaliculatus[J]. 海洋湖沼学报（英文）, 2018,36(2):438-449. DOI： 10.1007/s00343-017-6235-x.

Xinxu ZHANG, Huijuan WU, Zhongzhen LI, et al. Effects of dietary supplementation of Ulva pertusa and nonstarch polysaccharide enzymes on gut microbiota of Siganus canaliculatus[J]. Chinese Journal of Oceanology and Limnology, 2018, 36(2): 438-449. DOI： 10.1007/s00343-017-6235-x.

摘要

Abstract

Fishes represent the highest diversity of vertebrates; however

our understanding of the compositions and functions of their gut microbiota is limited. In this study

we provided the first insight into the gut microbiota of the herbivorous fish

Siganus canaliculatus

by using three molecular ecology techniques based on the 16S rRNA genes (denaturing gradient gel electrophoresis

clone library construction

and highthroughput Illumina sequencing)

and the Illumina sequencing technique is suggested here due to its higher overall coverage of the total 16S rRNA genes. A core gut microbiota of 29 bacterial groups

covering

99.9% of the total bacterial community

was found to be dominated by Proteobacteria and Firmicutes in fish fed three different diets with/without the supplementation of

Ulva pertusa

and non-starch polysaccharide (NSP) enzymes (cellulase

xylanase

and β-glucanase). Diverse potential NSP-degrading bacteria and probiotics (e.g.

Ruminococcus

Clostridium

and Lachnospiraceae) were detected in the intestine of the fish fed

U. pertusa

suggesting that these microorganisms likely participated in the degradation of NSPs derived from

U. pertusa

. This study supports our previous conclusion that

U. pertusa

-based diets are suitable for the production of

S. canaliculatus

with lower costs without compromising quality.

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Keywords

references

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Effects of dietary supplementation of Ulva pertusa and nonstarch polysaccharide enzymes on gut microbiota of Siganus canaliculatus

DOI：10.1007/s00343-017-6235-x

摘要

Abstract

关键词

Keywords

references