Response of cellular stoichiometry and phosphorus storage of the cyanobacteria Aphanizomenon flos-aquae to small-scale turbulence

Zhe Li; Yan Xiao; Jixiang Yang; Chao Li; Xia Gao; Jinsong Guo

doi:10.1007/s00343-017-6178-2

Your Location：

Home >

Browse articles >

Response of cellular stoichiometry and phosphorus storage of the cyanobacteria Aphanizomenon flos-aquae to small-scale turbulence

OriginalPaper | Updated：2023-05-16

- Response of cellular stoichiometry and phosphorus storage of the cyanobacteria Aphanizomenon flos-aquae to small-scale turbulence
- Response of cellular stoichiometry and phosphorus storage of the cyanobacteria Aphanizomenon flos-aquae to small-scale turbulence
- 海洋湖沼学报（英文） 2017年35卷第6期页码：1409-1416
- Affiliations：
  
  1. CAS Key Laboratory on Reservoir Water Environment Chongqing Institute of Green and Intelligent Technology Chinese Academy of Sciences
  2. State Key Laboratory of Bioreactor Engineering College of Biotechnology East China University of Science and Technology
- Author bio：
- Funds：
  
  Supported by the National Natural Science Foundation of China (Nos. 51309220, 51679226), the National Key SandT Project on Water Pollution Control and Treatment (Nos. 2014ZX07104-006, 2015ZX07103-007), and the Western Action Program funded by the Chinese Academy of Sciences (No. KZCX2-XB3-14)
- DOI：10.1007/s00343-017-6178-2
  中图分类号：
- 网络首发：2017-01-26，
  
  纸质出版：2017
- Accepted：
Scan QR Code
Response of cellular stoichiometry and phosphorus storage of the cyanobacteria Aphanizomenon flos-aquae to small-scale turbulence[J]. 海洋湖沼学报（英文）, 2017,35(6):1409-1416.

Li, Z., Xiao, Y., Yang, J. et al. Response of cellular stoichiometry and phosphorus storage of the cyanobacteria Aphanizomenon flos-aquae to small-scale turbulence., Chin. J. Ocean. Limnol. 35, 1409–1416 (2017). https://doi.org/10.1007/s00343-017-6178-2
Response of cellular stoichiometry and phosphorus storage of the cyanobacteria Aphanizomenon flos-aquae to small-scale turbulence[J]. 海洋湖沼学报（英文）, 2017,35(6):1409-1416. DOI： 10.1007/s00343-017-6178-2.

Li, Z., Xiao, Y., Yang, J. et al. Response of cellular stoichiometry and phosphorus storage of the cyanobacteria Aphanizomenon flos-aquae to small-scale turbulence., Chin. J. Ocean. Limnol. 35, 1409–1416 (2017). https://doi.org/10.1007/s00343-017-6178-2 DOI：

摘要

Abstract

Turbulent mixing

in particular on a small scale

affects the growth of microalgae by changing diffusive sublayers and regulating nutrient fluxes of cells. We tested the nutrient flux hypothesis by evaluating the cellular stoichiometry and phosphorus storage of microalgae under different turbulent mixing conditions. Aphanizomenon flos-aquae were cultivated in different stirring batch reactors with turbulent dissipation rates ranging from 0.001 51 m2/s3 to 0.050 58 m2/s3

the latter being the highest range observed in natural aquatic systems. Samples were taken in the exponential growth phase and compared with samples taken when the reactor was completely stagnant. Results indicate that

within a certain range

turbulent mixing stimulates the growth of A. flos-aquae. An inhibitory effect on growth rate was observed at the higher range. Photosynthesis activity

in terms of maximum effective quantum yield of PSII (the ratio of Fv/Fm) and cellular chlorophyll a

did not change significantly in response to turbulence. However

Chl a/C mass ratio and C/N molar ratio

showed a unimodal response under a gradient of turbulent mixing

similar to growth rate. Moreover

we found that increases in turbulent mixing might stimulate respiration rates

which might lead to the use of polyphosphate for the synthesis of cellular constituents. More research is required to test and verify the hypothesis that turbulent mixing changes the diffusive sublayer

regulating the nutrient flux of cells.

关键词

Keywords

references

Ayata S D, Lévy M, Aumont O, Resplandy L, Tagliabue A, Sciandra A, Bernard O. 2014. Phytoplankton plasticity drives large variability in carbon fixation efficiency. Geophys. Res. Lett., 41(24): 8994–9000.

Dickman E M, Vanni M J, Horgan M J. 2006. Interactive effects of light and nutrients on phytoplankton stoichiometry. Oecologia, 149(4): 676–689.

Ebert U, Arrayás M, Temme N, Sommeijer B, Huisman J. 2001. Critical conditions for phytoplankton blooms. Bull. Math. Biol., 63(6): 1095–1124.

Eixler S, Karsten U, Selig U. 2006. Phosphorus storage in Chlorella vulgaris (Trebouxiophyceae, Chlorophyta) cells and its dependence on phosphate supply. Phycologia, 45(1): 53–60.

Eixler S, Selig U, Karsten U. 2005. Extraction and detection methods for polyphosphate storage in autotrophic planktonic organisms. Hydrobiologia, 533(1-3): 135–143.

Elliott J A. 2010. The seasonal sensitivity of Cyanobacteria and other phytoplankton to changes in flushing rate and water temperature. Global Change Biol., 16(2): 864–876.

Estrada M, Berdalet E. 1997. Phytoplankton in a turbulent world. Scientia Marina, 61 (S1): 125–140.

Gallardo Rodríguez J J, Sánchez Mirón A, García Camacho F, Cerón García M C, Belarbi E H, Chisti Y, Molina Grima E. 2009. Causes of shear sensitivity of the toxic dinoflagellate Protoceratium reticulatum. Biote chnology Progress, 25(3): 792–800.

Guillard R R L. 1973. Division rates. In: Stein J R ed. Handbook of Phycological Methods. I. Culture Methods and Growth Measurements. Cambridge University Press, Cambridge. p.289–312.

Halsey K H, Jones B M. 2015. Phytoplankton strategies for photosynthetic energy allocation. Annu. Rev. Mar. Sci., 7(1): 265–297.

Halsey K H, Milligan A J, Behrenfeld M J. 2014. Contrasting strategies of photosynthetic energy utilization drive lifestyle strategies in ecologically important picoeukaryotes. Metabolites, 4(2): 260–280.

Hondzo M, Lyn D. 1999. Quantified small-scale turbulence inhibits the growth of a green alga. Freshwater Biol ogy, 41(1): 51–61.

Hondzo M, Wüest A. 2009. Do Microscopic organisms feel turbulent flows? Environ. Sci. Technol., 43(3): 764–768.

Huisman J, Arrayás M, Ebert U, Sommeijer B. 2002. How do sinking phytoplankton species manage to persist? Am. Nat., 159(3): 245–254.

Karp-Boss L, Boss E, Jumars P A. 1996. Nutrient fluxes to planktonic osmotrophs in the presence of fluid motion. Oceanogr. Mar. Biol. Annu. Rev, 34: 71–107.

Klausmeier C A, Litchman E, Daufresne T, Levin S A. 2008. Phytoplankton stoichiometry. Ecol. Res., 23(3): 479–485.

Leupold M, Hindersin S, Gust G, Kerner M, Hanelt D. 2013. Influence of mixing and shear stress on Chlorella vulgaris, Scenedesmus obliquus, and Chlamydomonas reinhardtii. J. Appl. Phycol., 25(2): 485–495.

Litchman E, Klausmeier C A. 2008. Trait-based community ecology of phytoplankton. Ann. Rev. Ecol. Evol. Syst., 39(1): 615–639.

Maxwell K, Johnson G N. 2000. Chlorophyll fluorescence—a practical guide. J. Exp. Bot., 51(345): 659–668.

Michels M H A, van der Goot A J, Norsker N H, Wijffels R H. 2010. Effects of shear stress on the microalgae Chaetoceros muelleri. Bioproc. Biosyst. Eng., 33(8): 921–927.

Montechiaro F, Giordano M. 2010. Compositional homeostasis of the dinoflagellate Protoceratium reticulatum grown at three different pCO2. J. Plant Physiol., 167(2): 110–113.

O’Halloran I P, Cade-Menun B J. 2008. Total and organic phosphorus. In: Carter M R, Gregorich E G eds. Soil Sampling and Methods of Analysis. 2 nd edn. CRC Press, Boca Raton.

Padisák J, Köhler J, Hoeg S. 1999. Effect of changing flushing rates on development of late summer Aphanizomenon and Microcystis populations in a shallow lake, Müggelsee, Berlin, Germany. In: Tundisi J G, Straškraba M eds. Theoretical Reservoir Ecology and Its Applications. Backhuys Publishers, Leiden. p.411–424.

Parkinson J A, Allen S E. 1975. A wet oxidation procedure suitable for the determination of nitrogen and mineral nutrients in biological material. Commun. Soil Sci. Plan t Anal., 6(1): 1–11.

Powell N, Shilton A N, Pratt S, Chisti Y. 2008. Factors influencing luxury uptake of phosphorus by microalgae in waste stabilization ponds. Environ. Sci. Technol., 42(16): 5958–5962.

Powell N, Shilton A, Chisti Y, Pratt S. 2009. Towards a luxury uptake process via microalgae-defining the polyphosphate dynamics. Water Res., 43(17): 4207–4213.

Reynolds C S. 2006. The Ecology of Phytoplankton. Cambridge University Press, Cambridge.

Rothschild B J, Osborn T R. 1988. Small-scale turbulence and plankton contact rates. J. Plankton Res., 10(3): 465–474.

Sterner R W, Elser J J. 2002. Ecological Stoichiometry: The Biology of Elements from Molecules to the Biosphere. Princeton University Press, Princeton.

Thomas W H, Gibson C H. 1990. Effects of small-scale turbulence on microalgae. J. Appl. Phycol., 2(1): 71–77.

Thomas W H, Vernet M, Gibson C H. 1995. Effects of smallscale turbulence on photosynthesis, pigmentation, cell division, and cell size in the marine dinoflagellate Gomaulax polyedra (Dinophyceae). J. Phycol., 31(1): 50–59.

Tripathi K, Sharma N K, Kageyama H, Takabe T, Rai A K. 2013. Physiological, biochemical and molecular responses of the halophilic cyanobacterium Aphanothece halophytica to Pi-deficiency. Eur. J. Phycol., 48(4): 461–473.

Warnaars T A, Hondzo M. 2006. Small-scale fluid motion mediates growth and nutrient uptake of Selenastrum capricornutum. Freshwater Biol ogy, 51(6): 999–1015.

Wintermans J F G M, de Mots A. 1965. Spectrophotometric characteristics of chlorophylls a and b and their phenophytins in ethanol. Biochimica et Biophysica Acta (BBA)-Biophysics including Photosynthesis, 109(2): 448–453.

浏览量

Downloads

CSCD

文章被引用时，请邮件提醒。

Submit

工具集

关联资源

Comparison of the photo-acclimation potential of floating and benthic thalli of Sargassum horneri (Phaeophyta) during autumn and winter

Harmful algal bloom-forming dinoflagellate Prorocentrum donghaiense inhibits the growth and photosynthesis of seaweed Sargassum fusiformis embryos

The mechanism of a new type of modified clay controlling Phaeocystis globosa growth

Effect of high temperature on the balance between photosynthetic light absorption and energy utilization in Chlorella pyrenoidosa (Chlorophyceae)

Cladophora mats in a Crimean hypersaline lake: structure, dynamics, and inhabiting animals