Involvement of two glycoside hydrolase family 19 members in colony morphotype and virulence in Flavobacterium columnare

Zhang Xiaolin; Li Nan; Qin Ting; Huang Bei; Nie Pin

doi:10.1007/s00343-017-6160-z

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Involvement of two glycoside hydrolase family 19 members in colony morphotype and virulence in Flavobacterium columnare

OriginalPaper | Updated：2023-05-16

- Involvement of two glycoside hydrolase family 19 members in colony morphotype and virulence in Flavobacterium columnare
- Involvement of two glycoside hydrolase family 19 members in colony morphotype and virulence in Flavobacterium columnare
- 海洋湖沼学报（英文） 2017年35卷第6期页码：1511-1523
- Affiliations：
  
  1. State Key Laboratory of Freshwater Ecology and Biotechnology Institute of Hydrobiology Chinese Academy of Sciences
  2. University of Chinese Academy of Sciences
  3. College of Fisheries Jimei University
- Author bio：
- Funds：
  
  Supported by the Chinese Academy of Sciences (No. XDA08010207), the National Key Technology R&D Program of China (No. 2012BAD25B02), and the State Key Laboratory of Freshwater Ecology and Biotechnology (No. 2016FBZ04)
- DOI：10.1007/s00343-017-6160-z
  中图分类号：
- 网络首发：2016-12-01，
  
  纸质出版：2017
- Accepted：
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Involvement of two glycoside hydrolase family 19 members in colony morphotype and virulence in Flavobacterium columnare[J]. 海洋湖沼学报（英文）, 2017,35(6):1511-1523.

Zhang, X., Li, N., Qin, T. et al. Involvement of two glycoside hydrolase family 19 members in colony morphotype and virulence in Flavobacterium columnare., Chin. J. Ocean. Limnol. 35, 1511–1523 (2017). https://doi.org/10.1007/s00343-017-6160-z
Involvement of two glycoside hydrolase family 19 members in colony morphotype and virulence in Flavobacterium columnare[J]. 海洋湖沼学报（英文）, 2017,35(6):1511-1523. DOI： 10.1007/s00343-017-6160-z.

Zhang, X., Li, N., Qin, T. et al. Involvement of two glycoside hydrolase family 19 members in colony morphotype and virulence in Flavobacterium columnare., Chin. J. Ocean. Limnol. 35, 1511–1523 (2017). https://doi.org/10.1007/s00343-017-6160-z DOI：

摘要

Abstract

Flavobacterium columnare is the pathogenic agent of columnaris disease in aquaculture. Using a recently developed gene deletion strategy

two genes that encode the Glyco_hydro_19 domain (GH19 domain) containing proteins

ghd-1 and ghd-2

were deleted separately and together from the F. columnare G4 wild type strain. Surprisingly

the single-

Δghd-1 and Δghd-2

and double-gene mutants

Δghd-1 Δghd -2

all had rhizoid and non-rhizoid colony morphotypes

which we named Δghd-1

Δghd-2

Δghd-1 Δghd-2

and NΔghd-1

NΔghd-2

and NΔghd-1 Δghd-2. However

chitin utilization was not detected in either these mutants or in the wild type. Instead

skimmed milk degradation was observed for the mutants and the wild type; the non-rhizoid strain NΔghd-2 exhibited higher degradation activity as revealed by the larger transparent circle on the skimmed milk plate. Using zebrafish as the model organism

we found that non-rhizoid mutants had higher LD50 values and were less virulent because zebrafish infected with these survived longer. Transcriptome analysis between the non-rhizoid and rhizoid colony morphotypes of each mutant

i.e.

NΔ ghd -1 versus (vs) Δghd-1

NΔghd-2 vs Δghd-2

and NΔghd-1 Δghd-2 vs Δghd-1 Δghd-2

revealed a large number of differentially expressed genes

among which 39 genes were common in three of the pairs compared. Although most of these genes encode hypothetical proteins

a few molecules such as phage tail protein

rhs element Vgr protein

thiol-activated cytolysin

and TonB-dependent outer membrane receptor precursor

expression of which was down-regulated in non-rhizoid mutants but up-regulated in rhizoid mutants

may play a role F. columnare virulence.

关键词

Keywords

references

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