Simultaneous identification and quantification of tetrodotoxin in fresh pufferfish and pufferfish-based products using immunoaffinity columns and liquid chromatography/quadrupole-linear ion trap mass spectrometry

Mengmeng Guo; Haiyan Wu; Tao Jiang; Zhijun Tan; Chunxia Zhao; Guanchao Zheng; Zhaoxin Li; Yuxiu Zhai

doi:10.1007/s00343-017-6097-2

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Simultaneous identification and quantification of tetrodotoxin in fresh pufferfish and pufferfish-based products using immunoaffinity columns and liquid chromatography/quadrupole-linear ion trap mass spectrometry

OriginalPaper | Updated：2023-05-16

- Simultaneous identification and quantification of tetrodotoxin in fresh pufferfish and pufferfish-based products using immunoaffinity columns and liquid chromatography/quadrupole-linear ion trap mass spectrometry
- Simultaneous identification and quantification of tetrodotoxin in fresh pufferfish and pufferfish-based products using immunoaffinity columns and liquid chromatography/quadrupole-linear ion trap mass spectrometry
- 海洋湖沼学报（英文） 2017年35卷第4期页码：883-893
- Affiliations：
  
  1. Key Laboratory of Testing and Evaluation for Aquatic Product Safety and Quality Ministry of Agriculture Chinese Academy of Fishery Sciences
  2. National Center for Quality Supervision and Test of Aquatic Products
  3. Yellow Sea Fisheries Research Institute Chinese Academy of Fishery Sciences
  4. Key Laboratory of Food Safety Risk Assessment Ministry of Health China National Center for Food Safety Risk Assessment
- Author bio：
- Funds：
  
  Supported by the National Natural Science Foundation of China (No. 41106109) and the China National Food Safety Standards Development Project (No. ZHENGHE-2015-356)
- DOI：10.1007/s00343-017-6097-2
  中图分类号：
- 网络首发：2016-08-12，
  
  纸质出版：2017
- Accepted：
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Simultaneous identification and quantification of tetrodotoxin in fresh pufferfish and pufferfish-based products using immunoaffinity columns and liquid chromatography/quadrupole-linear ion trap mass spectrometry[J]. 海洋湖沼学报（英文）, 2017,35(4):883-893.

Guo, M., Wu, H., Jiang, T. et al. Simultaneous identification and quantification of tetrodotoxin in fresh pufferfish and pufferfish-based products using immunoaffinity columns and liquid chromatography/quadrupole-linear ion trap mass spectrometry., Chin. J. Ocean. Limnol. 35, 883–893 (2017). https://doi.org/10.1007/s00343-017-6097-2
Simultaneous identification and quantification of tetrodotoxin in fresh pufferfish and pufferfish-based products using immunoaffinity columns and liquid chromatography/quadrupole-linear ion trap mass spectrometry[J]. 海洋湖沼学报（英文）, 2017,35(4):883-893. DOI： 10.1007/s00343-017-6097-2.

Guo, M., Wu, H., Jiang, T. et al. Simultaneous identification and quantification of tetrodotoxin in fresh pufferfish and pufferfish-based products using immunoaffinity columns and liquid chromatography/quadrupole-linear ion trap mass spectrometry., Chin. J. Ocean. Limnol. 35, 883–893 (2017). https://doi.org/10.1007/s00343-017-6097-2 DOI：

摘要

Abstract

In this study

we established a comprehensive method for simultaneous identification and quantification of tetrodotoxin (TTX) in fresh pufferfish tissues and pufferfish-based products using liquid chromatography/quadrupole-linear ion trap mass spectrometry (LC-QqLIT-MS). TTX was extracted by 1% acetic acid-methanol

and most of the lipids were then removed by freezing lipid precipitation

followed by purification and concentration using immunoaffinity columns (IACs). Matrix effects were substantially reduced due to the high specificity of the IACs

and thus

background interference was avoided. Quantitation analysis was therefore performed using an external calibration curve with standards prepared in mobile phase. The method was evaluated by fortifying samples at 1

and 100 ng/g

respectively

and the recoveries ranged from 75.8%–107%

with a relative standard deviation of less than 15%. The TTX calibration curves were linear over the range of 1–1 000 μg/L

with a detection limit of 0.3 ng/g and a quantification limit of 1 ng/g. Using this method

samples can be further analyzed using an information-dependent acquisition (IDA) experiment

in the positive mode

from a single liquid chromatography-tandem mass spectrometry injection

which can provide an extra level of confirmation by matching the full product ion spectra acquired for a standard sample with those from an enhanced product ion (EPI) library. The scheduled multiple reaction monitoring method enabled TTX to be screened for

and TTX was positively identified using the IDA and EPI spectra. This method was successfully applied to analyze a total of 206 samples of fresh pufferfish tissues and pufferfish-based products. The results from this study show that the proposed method can be used to quantify and identify TTX in a single run with excellent sensitivity and reproducibility

and is suitable for the analysis of complex matrix pufferfish samples.

关键词

Keywords

references

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