Structure-based function prediction of the expanding mollusk tyrosinase family

Ronglian Huang; Li Li; Guofan Zhang

doi:10.1007/s00343-017-6066-9

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Structure-based function prediction of the expanding mollusk tyrosinase family

OriginalPaper | Updated：2023-05-16

- Structure-based function prediction of the expanding mollusk tyrosinase family
- Structure-based function prediction of the expanding mollusk tyrosinase family
- 海洋湖沼学报（英文） 2017年35卷第6期页码：1454-1464
- Affiliations：
  
  1. Key Laboratory of Experimental Marine Biology Institute of Oceanology Chinese Academy of Sciences
  2. University of Chinese Academy of Sciences
  3. Marine Pearl Culture Lab Fishery College GuangDong Ocean University
  4. Laboratory for Marine Biology and Biotechnology Qingdao National Laboratory for Marine Science and Technology
- Author bio：
- Funds：
  
  Supported by the National Natural Science Foundation of China (No. 31530079), the Western Pacific Ocean System: Structure, Dynamics and Consequences (No. XDA11000000), the Technological Innovation Project (No. 2015ASKJ02-03, financially supported by Qingdao National Laboratory for Marine Science and Technology), and the Earmarked Fund for Modern Agro-Industry Technology Research System (No. CARS-48)
- DOI：10.1007/s00343-017-6066-9
  中图分类号：
- 网络首发：2017-01-26，
  
  纸质出版：2017
- Accepted：
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Structure-based function prediction of the expanding mollusk tyrosinase family[J]. 海洋湖沼学报（英文）, 2017,35(6):1454-1464.

Huang, R., Li, L. & Zhang, G. Structure-based function prediction of the expanding mollusk tyrosinase family., Chin. J. Ocean. Limnol. 35, 1454–1464 (2017). https://doi.org/10.1007/s00343-017-6066-9
Structure-based function prediction of the expanding mollusk tyrosinase family[J]. 海洋湖沼学报（英文）, 2017,35(6):1454-1464. DOI： 10.1007/s00343-017-6066-9.

Huang, R., Li, L. & Zhang, G. Structure-based function prediction of the expanding mollusk tyrosinase family., Chin. J. Ocean. Limnol. 35, 1454–1464 (2017). https://doi.org/10.1007/s00343-017-6066-9 DOI：

摘要

Abstract

Tyrosinase (Ty) is a common enzyme found in many different animal groups. In our previous study

genome sequencing revealed that the Ty family is expanded in the Pacific oyster (Crassostrea gigas). Here

we examine the larger number of Ty family members in the Pacific oyster by high-level structure prediction to obtain more information about their function and evolution

especially the unknown role in biomineralization. We verified 12 Ty gene sequences from Crassostrea gigas genome and Pinctada fucata martensii transcriptome. By using phylogenetic analysis of these Tys with functionally known Tys from other molluscan species

eight subgroups were identified (CgTy_s1

CgTy_s2

MolTy_s1

MolTy-s2

MolTy-s3

PinTy-s1

PinTy-s2 and PviTy). Structural data and surface pockets of the dinuclear copper center in the eight subgroups of molluscan Ty were obtained using the latest versions of prediction online servers. Structural comparison with other Ty proteins from the protein databank revealed functionally important residues (HA1

HA2

HA3

HB1

HB2

HB3

Z1–Z9) and their location within these protein structures. The structural and chemical features of these pockets which may related to the substrate binding showed considerable variability among mollusks

which undoubtedly defines Ty substrate binding. Finally

we discuss the potential driving forces of Ty family evolution in mollusks. Based on these observations

we conclude that the Ty family has rapidly evolved as a consequence of substrate adaptation in mollusks.

关键词

Keywords

references

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