Optimizing production of asperolide A, a potential anti-tumor tetranorditerpenoid originally produced by the algal-derived endophytic fungus Aspergillus wentii EN-48

Rui Xu; Xiaoming Li; Gangming Xu; Bingui Wang

doi:10.1007/s00343-017-6028-2

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Optimizing production of asperolide A, a potential anti-tumor tetranorditerpenoid originally produced by the algal-derived endophytic fungus Aspergillus wentii EN-48

OriginalPaper | Updated：2023-05-16

- Optimizing production of asperolide A, a potential anti-tumor tetranorditerpenoid originally produced by the algal-derived endophytic fungus Aspergillus wentii EN-48
- Optimizing production of asperolide A, a potential anti-tumor tetranorditerpenoid originally produced by the algal-derived endophytic fungus Aspergillus wentii EN-48
- 海洋湖沼学报（英文） 2017年35卷第3期页码：658-663
- Affiliations：
  
  1. Laboratory of Marine Biology and Biotechnology Qingdao National Laboratory for Marine Science and Technology Key Laboratory of Experimental Marine Biology Institute of Oceanology Chinese Academy of Sciences
  2. University of Chinese Academy of Sciences
- Author bio：
- Funds：
  
  Supported by the National Natural Science Foundation of China (NSFC, Nos. 31200042, 31570038) and the NSFC-Shandong Joint Fund for Marine Science Research Centers (No. U1406402)
- DOI：10.1007/s00343-017-6028-2
  中图分类号：
- 网络首发：2016-07-28，
  
  纸质出版：2017
- Accepted：
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Optimizing production of asperolide A, a potential anti-tumor tetranorditerpenoid originally produced by the algal-derived endophytic fungus Aspergillus wentii EN-48[J]. 海洋湖沼学报（英文）, 2017,35(3):658-663.

Xu, R., Li, X., Xu, G. et al. Optimizing production of asperolide A, a potential anti-tumor tetranorditerpenoid originally produced by the algal-derived endophytic fungus Aspergillus wentii EN-48., Chin. J. Ocean. Limnol. 35, 658–663 (2017). https://doi.org/10.1007/s00343-017-6028-2
Optimizing production of asperolide A, a potential anti-tumor tetranorditerpenoid originally produced by the algal-derived endophytic fungus Aspergillus wentii EN-48[J]. 海洋湖沼学报（英文）, 2017,35(3):658-663. DOI： 10.1007/s00343-017-6028-2.

Xu, R., Li, X., Xu, G. et al. Optimizing production of asperolide A, a potential anti-tumor tetranorditerpenoid originally produced by the algal-derived endophytic fungus Aspergillus wentii EN-48., Chin. J. Ocean. Limnol. 35, 658–663 (2017). https://doi.org/10.1007/s00343-017-6028-2 DOI：

摘要

Abstract

The marine algal-derived endophytic fungus Aspergillus wentii EN-48 produces the potential anti-tumor agent asperolide A

a tetranorlabdane diterpenoid active against lung cancer. However

the fermentation yield of asperolide A was very low and only produced in static cultures. Static fermentation conditions of A. wentii EN-48 were optimized employing response surface methodology to enhance the production of asperolide A. The optimized conditions resulted in a 13.9-fold yield enhancement

which matched the predicted value

and the optimized conditions were successfully used in scale-up fermentation for the production of asperolide A. Exogenous addition of plant hormones (especially 10 μmol/L methyl jasmonate) stimulated asperolide A production. To our knowledge

this is first optimized production of an asperolide by a marine-derived fungus. The optimization is Effective and valuable to supply material for further anti-tumor mechanism studies and preclinical evaluation of asperolide A and other norditerpenoids.

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references

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