Recovery of gonadal development in tiger puffer Takifugu rubripes after exposure to 17β-estradiol during early life stages

Peng Hu; Bin Liu; Zhen Meng; Xinfu Liu; Yudong Jia; Zhi Yang; Jilin Lei

doi:10.1007/s00343-017-6016-6

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Recovery of gonadal development in tiger puffer Takifugu rubripes after exposure to 17β-estradiol during early life stages

OriginalPaper | Updated：2023-05-16

- Recovery of gonadal development in tiger puffer Takifugu rubripes after exposure to 17β-estradiol during early life stages
- Recovery of gonadal development in tiger puffer Takifugu rubripes after exposure to 17β-estradiol during early life stages
- 海洋湖沼学报（英文） 2017年35卷第3期页码：613-623
- Affiliations：
  
  1. Key Laboratory of Mariculture of Ministry of Education Ocean University of China
  2. Municipal Key Laboratory of Marine Fish Breeding and Biotechnology Yellow Sea Fisheries Research Institute Chinese Academy of Fishery Sciences
  3. Tianyuan Aquaculture Co. Ltd. of Yantai Economic Development Zone
- Author bio：
- Funds：
  
  Supported by the China Agriculture Research System (No. CARS-50-G20), the National Natural Science Foundation of China (No. 31402284), and the National High Technology Research and Development Program of China (863 Program) (No. 2012AA10A413-2);An erratum to this article is available at;https://doi.org/10.1007/s00343-017-7000-x;.
- DOI：10.1007/s00343-017-6016-6
  中图分类号：
- 网络首发：2016-06-14，
  
  纸质出版：2017
- Accepted：
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Recovery of gonadal development in tiger puffer Takifugu rubripes after exposure to 17β-estradiol during early life stages[J]. 海洋湖沼学报（英文）, 2017,35(3):613-623.

Hu, P., Liu, B., Meng, Z. et al. Recovery of gonadal development in tiger puffer Takifugu rubripes after exposure to 17β-estradiol during early life stages., Chin. J. Ocean. Limnol. 35, 613–623 (2017). https://doi.org/10.1007/s00343-017-6016-6
Recovery of gonadal development in tiger puffer Takifugu rubripes after exposure to 17β-estradiol during early life stages[J]. 海洋湖沼学报（英文）, 2017,35(3):613-623. DOI： 10.1007/s00343-017-6016-6.

Hu, P., Liu, B., Meng, Z. et al. Recovery of gonadal development in tiger puffer Takifugu rubripes after exposure to 17β-estradiol during early life stages., Chin. J. Ocean. Limnol. 35, 613–623 (2017). https://doi.org/10.1007/s00343-017-6016-6 DOI：

摘要

Abstract

The aim of the present study was to investigate the long-term effects of 17β-estradiol (E2) exposure on gonadal development in the tiger puff er (Takifugu rubripes)

which has a genetic sex determination system of male homogametic XY-XX. Tiger puff er larvae were exposed to 1

10 and 100 μg/L E2 from 15 to 100 days post-hatch (dph) and then maintained in clean seawater until 400 dph. Changes in sex ratio

gonadal structure and gonadosomatic index (GSI) were monitored at 100

160

270 and 400 dph. Sex-associated single nucleotide polymorphism (SNP) markers were used to analyze the genetic sex of samples

except those at 100 dph. Exposure had a positive effect on the conversion of genetically male gonads into phenotypically female gonads at 100 dph. However

gonads from 60% of genetic XY males in the 1-μg/L E2 group and 100% in the 10-μg/L E2 group developed intersexual gonads at 160 dph; gonads of all genetic XY males in the two treatment groups reverted to testis by 270 dph. While 38%

57% and 44% of gonads of XY fish in the 100-μg/L E2 group reverted to intersexual gonads at 160

270 and 400 dph

respectively

none reverted to testis after E2 treatment. In addition

E2 exposure inhibited gonadal growth of both genetic sexes

as indicated by the clear dose-dependent decrease in GSI at 270 and 400 dph. The results showed that exposure to E2 during the early life stages of tiger puff er disrupted gonadal development

but that fish recovered after migration to clean seawater. The study suggests the potential use of tiger puff er as a valuable indicator species to evaluate the effects of environmental estrogens on marine fish

thereby protecting valuable fishery resources.

关键词

Keywords

references

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