Transcriptome profiles of embryos before and after cleavage in Eriocheir sinensis: identification of developmental genes at the earliest stages

Min Hui; Zhaoxia Cui; Yuan Liu; Chengwen Song

doi:10.1007/s00343-017-5364-6

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Transcriptome profiles of embryos before and after cleavage in Eriocheir sinensis: identification of developmental genes at the earliest stages

OriginalPaper | Updated：2023-05-16

- Transcriptome profiles of embryos before and after cleavage in Eriocheir sinensis: identification of developmental genes at the earliest stages
- Transcriptome profiles of embryos before and after cleavage in Eriocheir sinensis: identification of developmental genes at the earliest stages
- 海洋湖沼学报（英文） 2017年35卷第4期页码：770-781
- Affiliations：
  
  1. Key Laboratory of Experimental Marine Biology Institute of Oceanology Chinese Academy of Sciences
  2. National & Local Joint Engineering Laboratory of Ecological Mariculture
  3. Laboratory for Marine Biology and Biotechnology Qingdao National Laboratory for Marine Science and Technology
  4. University of Chinese Academy of Sciences
- Author bio：
- Funds：
  
  Supported by the National High Technology Research and Development Program of China (863 Program) (No. 2012AA10A409), the Scientific and Technological Innovation Project of Qingdao National Laboratory for Marine Science and Technology (No. 2015ASKJ02), and the National Natural Science Foundation of China (Nos. 41276165 to Dr. CUI Zhaoxia, 31302187 to Dr. HUI Min);Electronic supplementary material;Supplementary material (Supplementary Tables 1–2 and Supplementary Fig.1) is available in the online version of this article at http://dx.doi.org/10.1007/s00343-017-5364-6.
- DOI：10.1007/s00343-017-5364-6
  中图分类号：
- 网络首发：2016-09-08，
  
  纸质出版：2017
- Accepted：
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Transcriptome profiles of embryos before and after cleavage in Eriocheir sinensis: identification of developmental genes at the earliest stages[J]. 海洋湖沼学报（英文）, 2017,35(4):770-781.

Hui, M., Cui, Z., Liu, Y. et al. Transcriptome profiles of embryos before and after cleavage in Eriocheir sinensis: identification of developmental genes at the earliest stages., Chin. J. Ocean. Limnol. 35, 770–781 (2017). https://doi.org/10.1007/s00343-017-5364-6
Transcriptome profiles of embryos before and after cleavage in Eriocheir sinensis: identification of developmental genes at the earliest stages[J]. 海洋湖沼学报（英文）, 2017,35(4):770-781. DOI： 10.1007/s00343-017-5364-6.

Hui, M., Cui, Z., Liu, Y. et al. Transcriptome profiles of embryos before and after cleavage in Eriocheir sinensis: identification of developmental genes at the earliest stages., Chin. J. Ocean. Limnol. 35, 770–781 (2017). https://doi.org/10.1007/s00343-017-5364-6 DOI：

摘要

Abstract

In crab

embryogenesis is a complicated developmental program marked by a series of critical events. RNA-Sequencing technology offers developmental biologists a way to identify many more developmental genes than ever before. Here

we present a comprehensive analysis of the transcriptomes of Eriocheir sinensis oosperms (Os) and embryos at the 2–4 cell stage (Cs)

which are separated by a cleavage event. A total of 18 923 unigenes were identified

and 403 genes matched with gene ontology (GO) terms related to developmental processes. In total

432 differentially expressed genes (DEGs) were detected between the two stages. Nine DEGs were specifically expressed at only one stage. These DEGs may be relevant to stage-specific molecular events during development. A number of DEGs related to ‘hedgehog signaling pathway’

‘Wnt signaling pathway’ ‘germplasm’

‘nervous system’

‘sensory perception’ and ‘segment polarity’ were identified as being up-regulated at the Cs stage. The results suggest that these embryonic developmental events begin before the early cleavage event in crabs

and that many of the genes expressed in the two transcriptomes might be maternal genes. Our study provides ample information for further research on the molecular mechanisms underlying crab development.

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