Thermal tolerance evaluation and related microsatellite marker screening and identification in the large yellow croaker Larimichthys crocea
OriginalPaper|Updated:2023-05-16
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Thermal tolerance evaluation and related microsatellite marker screening and identification in the large yellow croaker Larimichthys crocea
Thermal tolerance evaluation and related microsatellite marker screening and identification in the large yellow croaker Larimichthys crocea
海洋湖沼学报(英文)2017年35卷第3期 页码:566-571
Affiliations:
1. Key Laboratory of Mariculture for the East China Sea Ministry of Agriculture Jimei University
2. Fisheries College Jimei University
Author bio:
Funds:
Supported by the National Natural Science Foundation of China (No. 31172397) and the New Century Excellent Talents of Fujian University (Nos. JA14167, C18013)
Thermal tolerance evaluation and related microsatellite marker screening and identification in the large yellow croaker Larimichthys crocea[J]. 海洋湖沼学报(英文), 2017,35(3):566-571.
Chen, X., Li, J., Wang, Z. et al. Thermal tolerance evaluation and related microsatellite marker screening and identification in the large yellow croaker Larimichthys crocea, ., Chin. J. Ocean. Limnol. 35, 566–571 (2017). https://doi.org/10.1007/s00343-017-5342-z
Thermal tolerance evaluation and related microsatellite marker screening and identification in the large yellow croaker Larimichthys crocea[J]. 海洋湖沼学报(英文), 2017,35(3):566-571. DOI: 10.1007/s00343-017-5342-z.
Chen, X., Li, J., Wang, Z. et al. Thermal tolerance evaluation and related microsatellite marker screening and identification in the large yellow croaker Larimichthys crocea, ., Chin. J. Ocean. Limnol. 35, 566–571 (2017). https://doi.org/10.1007/s00343-017-5342-zDOI:
Thermal tolerance evaluation and related microsatellite marker screening and identification in the large yellow croaker Larimichthys crocea
摘要
Abstract
Thermal tolerance to high temperature was evaluated in the large yellow croaker Larimichthys crocea. The survival thermal maximum for L. crocea was 33.0°C
the 50% critical thermal maximum (50% CTMax) was 35.5°C
and the critical thermal maximum (CTMax) was 36.0°C. Three microsatellite markers (LYC0148
LYC0200 and LYC0435)
associated with thermal tolerance were screened and identified using a Bulked Segregation Analysis (BSA) method. These markers have six amplified fragments in which four are related to thermal tolerance. These fragments were cloned and sequenced
and the results showed the core motif were all “AC” repeats. For LYC0148 and LYC0200
the lengths of fragments are 181 bp and 197 bp
respectively. For LYC0435
which has two fragments
the fragment lengths are 112 bp and 100 bp. The results provide useful molecular markers for thermal-tolerance breeding of large yellow croaker in the near future.
关键词
Keywords
references
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相关作者
Hailing YANG
Fangzhu WU
Fangping CHEN
Jiangning ZENG
Qikun YANG
Lukuo MA
Tianqi ZHAI
Yu ZHOU
相关机构
Key Laboratory of Nearshore Engineering Environment and Ecological Security of Zhejiang Province, Second Institute of Oceanography, Ministry of Natural Resources
Shelf Sea System Ecology, Alfred Wegener Institute Helmholtz Center for Polar and Marine Research, Helgoland
Key Laboratory of Ocean Space Resource Management Technology, Ministry of Natural Resources
Key Laboratory of Marine Ecosystem Dynamics, Second Institute of Oceanography, Ministry of Natural Resources
Key Laboratory of Applied Marine Biotechnology by the Ministry of Education, School of Marine Sciences, Ningbo University