Intestinal disease of scattered mirror carp Cyprinus carpio caused by Thelohanellus kitauei and notes on the morphology and phylogeny of the myxosporean from Sichuan Province, southwest China

Lingtong Ye; Mingmiao Lu; Keyan Quan; Wenxiang Li; Hong Zou; Shangong Wu; Jiangyong Wang; Guitang Wang

doi:10.1007/s00343-017-5312-5

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Intestinal disease of scattered mirror carp Cyprinus carpio caused by Thelohanellus kitauei and notes on the morphology and phylogeny of the myxosporean from Sichuan Province, southwest China

OriginalPaper | Updated：2023-05-16

- Intestinal disease of scattered mirror carp Cyprinus carpio caused by Thelohanellus kitauei and notes on the morphology and phylogeny of the myxosporean from Sichuan Province, southwest China
- Intestinal disease of scattered mirror carp Cyprinus carpio caused by Thelohanellus kitauei and notes on the morphology and phylogeny of the myxosporean from Sichuan Province, southwest China
- 海洋湖沼学报（英文） 2017年35卷第3期页码：587-596
- Affiliations：
  
  1. Key Laboratory of Fishery Ecology and Environment Guangdong Province
  2. Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization Ministry of Agriculture
  3. South China Sea Fisheries Research Institute Chinese Academy of Fishery Sciences
  4. Key Laboratory of Aquatic Biodiversity and Conservation and the Institute of Hydrobiology Chinese Academy of Sciences
  5. Sichuan Fisheries School
- Author bio：
- Funds：
  
  Supported by the earmarked fund for China Agriculture Research System (No. CARS-46-08), the National Science Foundation for Young Scientists of China (No. 31301863), and the Major Scientific and Technological Innovation Project of Hubei Province (No. 2015ABA045)
- DOI：10.1007/s00343-017-5312-5
  中图分类号：
- 网络首发：2016-05-26，
  
  纸质出版：2017
- Accepted：
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Intestinal disease of scattered mirror carp Cyprinus carpio caused by Thelohanellus kitauei and notes on the morphology and phylogeny of the myxosporean from Sichuan Province, southwest China[J]. 海洋湖沼学报（英文）, 2017,35(3):587-596.

Ye, L., Lu, M., Quan, K. et al. Intestinal disease of scattered mirror carp Cyprinus carpio caused by Thelohanellus kitauei and notes on the morphology and phylogeny of the myxosporean from Sichuan Province, southwest China., Chin. J. Ocean. Limnol. 35, 587–596 (2017). https://doi.org/10.1007/s00343-017-5312-5
Intestinal disease of scattered mirror carp Cyprinus carpio caused by Thelohanellus kitauei and notes on the morphology and phylogeny of the myxosporean from Sichuan Province, southwest China[J]. 海洋湖沼学报（英文）, 2017,35(3):587-596. DOI： 10.1007/s00343-017-5312-5.

Ye, L., Lu, M., Quan, K. et al. Intestinal disease of scattered mirror carp Cyprinus carpio caused by Thelohanellus kitauei and notes on the morphology and phylogeny of the myxosporean from Sichuan Province, southwest China., Chin. J. Ocean. Limnol. 35, 587–596 (2017). https://doi.org/10.1007/s00343-017-5312-5 DOI：

摘要

Abstract

The mass mortality of pond-reared scattered mirror carp

Cyprinus carpio

caused by Thelohanellus kitauei

occurred at fish farms in Sichuan Province

southwest China. Morphological and molecular analyses were supplemented with histological evaluation of infected tissues to better understand the route of infection and the pathological effects of T. kitauei on the fish host. The intestine of the diseased host was full of large cysts of the myxosporean. The cysts range from 2 cm to 3.6 cm in diameter. Histopathology indicated that T. kitaue i first invaded the submucosa of the host intestine and then moved into the mucosa layers with the development of their spores

finally entering into the enteric cavity of the hosts after the disruption of mucosa layers. The pyriform spores of T. kitauei were surrounded by the transparent spore sheath

measuring 25.98 μm±0.95 μm in body length

8.72 μm±0.51 μm in body width

and 7.86 μm±0.26 μm in body thickness. The single polar capsule was pyriform

measuring 14.73 μm±0.92 μm in length and 6.82 μm±0.45 μm in width

with eight to 10 turns of filament coils winding inside. Phylogenetic analysis based on the 18S small-subunit ribosomal DNA sequences indicated that minimal genetic differences were present between T. kitauei samples from South Korea and from China. Close affinity was found between the genus Thelohanellus and Myxobolus. Additionally

two polar capsule nuclei were found at the anterior end of the single polar capsule in spores of T. kitauei stained with hematoxylin and eosin

which suggested the separation of the genus Thelohanellus from Myxobolus.

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references

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