Comparative transcriptomics reveals genes involved in metabolic and immune pathways in the digestive gland of scallop Chlamys farreri following cadmium exposure

Hui Zhang; Yuxiu Zhai; Lin Yao; Yanhua Jiang; Fengling Li

doi:10.1007/s00343-017-5305-4

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Comparative transcriptomics reveals genes involved in metabolic and immune pathways in the digestive gland of scallop Chlamys farreri following cadmium exposure

OriginalPaper | Updated：2023-05-16

- Comparative transcriptomics reveals genes involved in metabolic and immune pathways in the digestive gland of scallop Chlamys farreri following cadmium exposure
- Comparative transcriptomics reveals genes involved in metabolic and immune pathways in the digestive gland of scallop Chlamys farreri following cadmium exposure
- 海洋湖沼学报（英文） 2017年35卷第3期页码：603-612
- Affiliations：
  
  1. Yellow Sea Fisheries Research Institute Chinese Academy of Fishery Sciences
  2. Key Laboratory of Testing and Evaluation for Aquatic Product Safety and Quality Ministry of Agriculture
  3. Laboratory of Quality and Safety Risk Assessment for Aquatic Products (Qingdao) Ministry of Agriculture
- Author bio：
- Funds：
  
  Supported by the Shandong Provincial Post-Doctoral Special Fund Innovative Project (No. S43201403), the Post-Doctoral Applied Research Project of Qingdao City (No. Q51201413), and the Shandong Provincial Natural Science Foundation, China (No. ZR2015PD004)
- DOI：10.1007/s00343-017-5305-4
  中图分类号：
- 网络首发：2016-05-26，
  
  纸质出版：2017
- Accepted：
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Comparative transcriptomics reveals genes involved in metabolic and immune pathways in the digestive gland of scallop Chlamys farreri following cadmium exposure[J]. 海洋湖沼学报（英文）, 2017,35(3):603-612.

Zhang, H., Zhai, Y., Yao, L. et al. Comparative transcriptomics reveals genes involved in metabolic and immune pathways in the digestive gland of scallop Chlamys farreri following cadmium exposure., Chin. J. Ocean. Limnol. 35, 603–612 (2017). https://doi.org/10.1007/s00343-017-5305-4
Comparative transcriptomics reveals genes involved in metabolic and immune pathways in the digestive gland of scallop Chlamys farreri following cadmium exposure[J]. 海洋湖沼学报（英文）, 2017,35(3):603-612. DOI： 10.1007/s00343-017-5305-4.

Zhang, H., Zhai, Y., Yao, L. et al. Comparative transcriptomics reveals genes involved in metabolic and immune pathways in the digestive gland of scallop Chlamys farreri following cadmium exposure., Chin. J. Ocean. Limnol. 35, 603–612 (2017). https://doi.org/10.1007/s00343-017-5305-4 DOI：

摘要

Abstract

Chlamys farreri is an economically important mollusk that can accumulate excessive amounts of cadmium (Cd). Studying the molecular mechanism of Cd accumulation in bivalves is difficult because of the lack of genome background. Transcriptomic analysis based on high-throughput RNA sequencing has been shown to be an efficient and powerful method for the discovery of relevant genes in non-model and genome reference-free organisms. Here

we constructed two cDNA libraries (control and Cd exposure groups) from the digestive gland of C. farreri and compared the transcriptomic data between them. A total of 227 673 transcripts were assembled into 105 071 unigenes

most of which shared high similarity with sequences in the NCBI non-redundant protein database. For functional classification

24 493 unigenes were assigned to Gene Ontology terms. Additionally

EuKaryotic Ortholog Groups and Kyoto Encyclopedia of Genes and Genomes analyses assigned 12 028 unigenes to 26 categories and 7 849 unigenes to five pathways

respectively. Comparative transcriptomics analysis identified 3 800 unigenes that were differentially expressed in the Cd-treated group compared with the control group. Among them

genes associated with heavy metal accumulation were screened

including metallothionein

divalent metal transporter

and metal tolerance protein. The functional genes and predicted pathways identified in our study will contribute to a better understanding of the metabolic and immune system in the digestive gland of C. farreri. In addition

the transcriptomic data will provide a comprehensive resource that may contribute to the understanding of molecular mechanisms that respond to marine pollutants in bivalves.

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references

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