Construction of a growth model in the green alga Tetraselmis subcordiformis using a response surface approach

Hui Wang; Jingyan Niu; Jiahui Liu; Hongshuai Yang; Zhigang Liu

doi:10.1007/s00343-017-5298-z

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Construction of a growth model in the green alga Tetraselmis subcordiformis using a response surface approach

OriginalPaper | Updated：2023-05-16

- Construction of a growth model in the green alga Tetraselmis subcordiformis using a response surface approach
- Construction of a growth model in the green alga Tetraselmis subcordiformis using a response surface approach
- 海洋湖沼学报（英文） 2017年35卷第3期页码：537-545
- Affiliations：
  
  1. Life Sciences College Huaiyin Normal University
  2. Polytechnic Department Jiaozuo Teachers College
  3. Fisheries College Guangdong Ocean University
- Author bio：
- Funds：
  
  Supported by the Guangdong Province Science and Technology Project (No. 2010B020201014), the Guangdong Province South China Sea Invertebrates Healthy Culture Engineering Technology Research Center (No. GCZX-A0909), the Guangdong Province Ocean and Fisheries Science and Technology Extension Project (No. 20120980), the Guangdong Province Industry-University-Science Partnership Project (No. 20110908), and the Sci & Tech Plan of Huaiyin Normal University (No. WH0031)
- DOI：10.1007/s00343-017-5298-z
  中图分类号：
- 网络首发：2016-06-29，
  
  纸质出版：2017
- Accepted：
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Construction of a growth model in the green alga Tetraselmis subcordiformis using a response surface approach[J]. 海洋湖沼学报（英文）, 2017,35(3):537-545.

Wang, H., Niu, J., Liu, J. et al. Construction of a growth model in the green alga Tetraselmis subcordiformis using a response surface approach., Chin. J. Ocean. Limnol. 35, 537–545 (2017). https://doi.org/10.1007/s00343-017-5298-z
Construction of a growth model in the green alga Tetraselmis subcordiformis using a response surface approach[J]. 海洋湖沼学报（英文）, 2017,35(3):537-545. DOI： 10.1007/s00343-017-5298-z.

Wang, H., Niu, J., Liu, J. et al. Construction of a growth model in the green alga Tetraselmis subcordiformis using a response surface approach., Chin. J. Ocean. Limnol. 35, 537–545 (2017). https://doi.org/10.1007/s00343-017-5298-z DOI：

摘要

Abstract

The green alga Tetraselmis subcordiformis has been widely used as a quality live food for aquaculture species

and also has been studied as a model organism for the photo-biological production of hydrogen. We attempted to quantify the relationship between T. subcordiformis specific growth rate (SGR) and three important environmental factors (temperature

salinity

and pH) using the central composite design and response surface method under laboratory conditions. The results showed that the linear effects of temperature and salinity were significant (P< 0.05)

and they were equally important in impacting T. subcordiformis specific growth; the linear effect of pH was not significant (P>0.05); the interactive effect of temperature and pH was significant (P<0.05)

whereas the temperature × salinity and salinity × pH interactions were not significant (P>0.05); all of the quadratic effects of the three factors were significant (P<0.05). A model equation for specific growth rate with the three factors was established

with the unadjusted and predictive R 2 as high as 0.990 and 0.921

respectively

suggesting that the model was a very good fit and that it could be used to predict SGR. Through optimizing the reliable model

an optimal 3-factor combination of 25 °C/35 of salinity/pH 7.9 was obtained

at which the maximum specific growth rate (0.65) was recorded

with a desirability value of 93.8%. These experimental results could serve as guidelines for increasing T. subcordiformis production efficiency.

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references

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