Characterization of dominant and cellulolytic bacterial communities along the gut of silver carp Hypophthalmichthys molitrix during cyanobacterial blooms

Congqiang Luo; Chunlong Yi; Leyi Ni; Longgen Guo

doi:10.1007/s00343-017-5253-z

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Characterization of dominant and cellulolytic bacterial communities along the gut of silver carp Hypophthalmichthys molitrix during cyanobacterial blooms

OriginalPaper | Updated：2023-05-16

- Characterization of dominant and cellulolytic bacterial communities along the gut of silver carp Hypophthalmichthys molitrix during cyanobacterial blooms
- Characterization of dominant and cellulolytic bacterial communities along the gut of silver carp Hypophthalmichthys molitrix during cyanobacterial blooms
- 海洋湖沼学报（英文） 2017年35卷第3期页码：624-633
- Affiliations：
  
  1. Donghu Experimental Station of Lake Ecosystems State Key Laboratory of Freshwater Ecology and Biotechnology of China Institute of Hydrobiology Chinese Academy of Sciences
  2. University of Chinese Academy of Sciences
- Author bio：
- Funds：
  
  Supported by the National Science and Technology Ministry (No. 2014BAC09B02) and the National Water Pollution Control and Management Technology Major Projects (No. 2012ZX07101-002)
- DOI：10.1007/s00343-017-5253-z
  中图分类号：
- 网络首发：2016-05-12，
  
  纸质出版：2017
- Accepted：
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Characterization of dominant and cellulolytic bacterial communities along the gut of silver carp Hypophthalmichthys molitrix during cyanobacterial blooms[J]. 海洋湖沼学报（英文）, 2017,35(3):624-633.

Luo, C., Yi, C., Ni, L. et al. Characterization of dominant and cellulolytic bacterial communities along the gut of silver carp Hypophthalmichthys molitrix during cyanobacterial blooms., Chin. J. Ocean. Limnol. 35, 624–633 (2017). https://doi.org/10.1007/s00343-017-5253-z
Characterization of dominant and cellulolytic bacterial communities along the gut of silver carp Hypophthalmichthys molitrix during cyanobacterial blooms[J]. 海洋湖沼学报（英文）, 2017,35(3):624-633. DOI： 10.1007/s00343-017-5253-z.

Luo, C., Yi, C., Ni, L. et al. Characterization of dominant and cellulolytic bacterial communities along the gut of silver carp Hypophthalmichthys molitrix during cyanobacterial blooms., Chin. J. Ocean. Limnol. 35, 624–633 (2017). https://doi.org/10.1007/s00343-017-5253-z DOI：

摘要

Abstract

Silver carp is one of the most important planktivorous fish in Chinese aquaculture and plays a significant role controlling cyanobacterial blooms. A balanced gut microbiota is crucial for growth and health of the host because of its important roles in immune defense

digestion of complex carbohydrates

and production of enterocytes. In our study

the dominant bacterial and cellulolytic bacterial (Clostridium I

Clostridium III

Clostridium XIVab

and Fibrobacter) communities in the contents and mucus of the silver carp gut (foregut

midgut

and hindgut) were analyzed by denaturing gradient gel electrophoresis and quantitative polymerase chain reaction (qPCR) analyses. The results revealed that the dominant and cellulolytic bacterial communities were significantly different among gut regions as well as in contents and mucus. Bacterial diversity and richness in contents and mucus increased along the gut and were higher in contents than those in local mucus. A sequence analysis of gut samples exhibited the conservative phylotypes of Proteobacteria

Actinobacteria

and Firmicutes. The gut of silver carp harbored an abundance of cellulolytic bacteria

particularly Clostridium XIV ab. The foregut segment had the highest proportions of the four cellulolytic bacteria

followed by the midgut and hindgut. However

the proportions of cellulolytic species in the silver carp gut was much lower than those in the terrestrial vertebrate gastrointestinal tract. We conclude that gut bacteria could help silver carp obtain energy from cyanobacteria

which may be why silver carp can maintain high growth rates during cyanobacterial blooms.

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references

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