Verification of protein sparing by feeding carbohydrate to common carp Cyprinus carpio

Zhenyan Cheng; Jinghui Li; Baolong Zhang; Zhenzhen Fang; Jinhui Sun; Dongqing Bai; Jinsheng Sun; Xiuting Qiao

doi:10.1007/s00343-016-5332-6

Your Location：

Home >

Browse articles >

Verification of protein sparing by feeding carbohydrate to common carp Cyprinus carpio

OriginalPaper | Updated：2023-05-16

- Verification of protein sparing by feeding carbohydrate to common carp Cyprinus carpio
- Verification of protein sparing by feeding carbohydrate to common carp Cyprinus carpio
- 海洋湖沼学报（英文） 2017年35卷第2期页码：251-257
- Affiliations：
  
  1. Tianjin Key Laboratory of Animal and Plant Resistance Tianjin Normal University
  2. Tianjin Key Lab of Aqua-Ecology and Aquaculture College of Fisheries Tianjin Agricultural University
- Author bio：
- Funds：
  
  Supported by the Key Technology R&D Program of Tianjin (No. 13ZCZDNC00900), the Tianjin Research Program of Application Foundation and Advanced Technology (No. 14JCQNJC15100), the Tianjin Innovative Research Team (No. TD12-5018), the Tianjin Key Laboratory of Animal and Plant Resistance, and the National Natural Science Foundation of China (No. 31402313);CHENG Zhenyan and LI Jinghui contributed equally to this work.
- DOI：10.1007/s00343-016-5332-6
  中图分类号：
- 网络首发：2016-06-14，
  
  纸质出版：2017
- Accepted：
Scan QR Code
Verification of protein sparing by feeding carbohydrate to common carp Cyprinus carpio[J]. 海洋湖沼学报（英文）, 2017,35(2):251-257.

Cheng, Z., Li, J., Zhang, B. et al. Verification of protein sparing by feeding carbohydrate to common carp Cyprinus carpio, ., Chin. J. Ocean. Limnol. 35, 251–257 (2017). https://doi.org/10.1007/s00343-016-5332-6
Verification of protein sparing by feeding carbohydrate to common carp Cyprinus carpio[J]. 海洋湖沼学报（英文）, 2017,35(2):251-257. DOI： 10.1007/s00343-016-5332-6.

Cheng, Z., Li, J., Zhang, B. et al. Verification of protein sparing by feeding carbohydrate to common carp Cyprinus carpio, ., Chin. J. Ocean. Limnol. 35, 251–257 (2017). https://doi.org/10.1007/s00343-016-5332-6 DOI：

摘要

Abstract

A 9-week feeding trial in floating freshwater cages (1.0 m×1.0 m×2.0 m) was conducted to study the effects of different dietary levels of protein and starch on growth

body composition

and gene expression of enzymes in common carp

Cyprinus carpio (mean body weight

36.12±1.18 g) to evaluate the protein-sparing effect of dietary carbohydrate. Four diets were formulated with corn starch as the carbohydrate source to obtain corn starch levels of 6.5%

13%

19.5%

or 26% and protein levels of 30.5%

28.2%

26.4%

and 24.2%. The results showed no differences in growth performance of fish fed the diets with different protein and corn starch levels

but body composition and glucose metabolic enzyme activity of carp were significantly affected by the different diets (P< 0.05). Weight gain

specific growth rate

and the feed conversion ratio were not different in fish fed the different dietary treatments. Protein efficiency ratio increased significantly as corn starch level increased (P< 0.05). Whole-body crude lipid composition increased with increasing dietary corn starch level (P< 0.05). Glucokinase (GK)

hexokinase

and pyruvate kinase (PK) activities increased significantly with increasing dietary corn starch level (P< 0.05)

whereas glucose-6-phosphate (G6Pase) activity decreased with increasing dietary corn starch level (P< 0.05). GK gene expression was significantly higher in fish fed the high-corn starch diet than those fed the low-corn starch diet (P< 0.05). G6pase gene expression tended to decrease with increasing starch level (P> 0.05). In summary

the results indicate a protein-sparing effect by substituting carbohydrate in the diet of common carp.

关键词

Keywords

references

Borrebaek B, Christophersen B. 2000. Hepatic glucose phosphorylating activities in perch (Perca fluviatilis) after different dietary treatments. Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, 125 (3): 387–393.

Cai C F, Wang Y L, Chen L Q, Yang C G, Ye Y T. 2006. Effects of level and seurce of dietary carbohydrate on growth and body composition of Mylopharyngodon piceus and Carassius auratus. Journal of Fishery Sciences of China, 13 (3): 452–459. (in Chinese with English abstract)

Capilla E, Médale F, Navarro I, Panserat S, Vachot C, Kaushik S, Gutiérrez J. 2003. Muscle insulin binding and plasma levels in relation to liver glucokinase activity, glucose metabolism and dietary carbohydrates in rainbow trout. Regulatory Peptides, 110 (2): 123–132.

Caseras A, Metón I, Vives C, Egea M, Fernández F, Baanante I V. 2002. Nutritional regulation of glucose-6-phosphatase gene expression in liver of the gilthead sea bream (Sparus aurata). British Journal of Nutrition, 88 (6): 607–614.

Cowey C B, Knox D, Walton M J, Adron J W. 1977. The regulation of gluconeogenesis by diet and insulin in rainbow trout (Salmo gairdneri). British Journal of Nutrition, 38 (3): 463–470.

Enes P, Panserat S, Kaushik S, Oliva-Teles A. 2006. Effect of normal and waxy maize starch on growth, food utilization and hepatic glucose metabolism in European sea bass (Dicentrarchus labrax) juveniles. Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology, 143 (1): 89–96.

Guo L, Jing R Z, Chen Z Y, Sun J H, Bai D Q, Qiao X T. 2013. A preliminary study of reducing carp feed protein. Feed Industry, 34 (8): 41–45. (in Chinese)

Hemre G I, Mommsen T P, Krogdahl Å. 2002. Carbohydrates in fish nutrition: effects on growth, glucose metabolism and hepatic enzymes. Aquaculture Nutrition, 8 (3): 175–194.

Keshavanath P, Manjappa K, Gangadhara B. 2002. Evaluation of carbohydrate rich diets through common carp culture in manured tanks. Aquaculture Nutrition, 8 (3): 169–174.

Krogdahl Å, Hemre G I, Mommsen T P. 2005. Carbohydrates in fish nutrition: digestion and absorption in postlarval stages. Aquaculture Nutrition, 11 (2): 103–122.

Metón I, Caseras A, Fernández F, Baanante I V. 2004. Molecular cloning of hepatic glucose-6-phosphatase catalytic subunit from gilthead sea bream (Sparus aurata): response of its mRNA levels and glucokinase expression to refeeding and diet composition. Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, 138 (2): 145–153.

Miao S Y, Miao H J, Nie Q, Zhang W B, Mai K S. 2013. Effects of different dietary carbohydrates on growth performance and metabolism response of juvenile turbot (Scophthalmus maximus). Journal of Fisheries of China, 37 (6): 910–919. (in Chinese with English abstract)

Mohapatra M, Sahu N P, Chaudhari A. 2003. Utilization of gelatinized carbohydrate in diets of Labeo rohita fry. Aquaculture Nutrition, 9 (3): 189–196.

NRC. 2011. Nutrient Requirements of Fish and Shrimp. National Academy Press, Washington DC. 128p.

Orire A M, Sadiku S O E. 2011. Protein sparing effects of lipids in the practical diets of Oreochromis niloticus (Nile tilapia). Nigerian Journal of Basic and Applied Sciences, 19 (1): 142–150.

Panserat S, Blin C, Médale F, Plagnes-Juan E, Brèque J, Krishnamoorthy J, Kaushik S. 2000b. Molecular cloning, tissue distribution and sequence analysis of complete glucokinase cDNAs from gilthead seabream (Sparus aurata), rainbow trout (Oncorhynchus mykiss) and common carp (Cyprinus carpio). Biochimica et Biophysica Acta (BBA)-General Subjects, 1474 (1): 61–69.

Panserat S, Fontagné S, Bergot P, Kaushik S. 2001. Ontogenesis of hexokinase I and hexokinase IV (glucokinase) gene expressions in common carp (Cyprinus carpio) related to diet. British Journal of Nutrition, 85 (6): 649–651.

Panserat S, Médale F, Blin C, Brèque J, Vachot C, Plagnes-Juan E, Gomes E, Krishnamoorthy R, Kaushik S. 2000a. Hepatic glucokinase is induced by dietary carbohydrates in rainbow trout, gilthead seabream, and common carp. American Journal of Physiology: Regulatory, Integrative Physiology, 278 (5): R1164–R1170.

Panserat S, Plagnes-Juan E, Kaushik S. 2002. Gluconeogenic enzyme gene expression is decreased by dietary carbohydrates in common carp (Cyprinus carpio) and gilthead seabream (Sparus aurata). Biochimica et Biophysica Acta (BBA)-Gene Structure and Expression, 1579 (1): 35–42.

Peres H, Oliva-Teles A. 2002. Utilization of raw and gelatinized starch by European sea bass (Dicentrarchus labrax) juveniles. Aquaculture, 205 (3-4): 287–299.

Polakof S, Skiba-Cassy S, Kaushik S, Seiliez I, Soengas J L, Panserat S. 2012. Glucose and lipid metabolism in the pancreas of rainbow trout is regulated at the molecular level by nutritional status and carbohydrate intake. Journal of Comparative Physiology B, 182 (4): 507–516.

Shiau S Y, Lin Y H. 2001. Carbohydrate utilization and its protein-sparing effect in diets for grouper (Epinephelus malabaricus). Animal Science, 73 (2): 299–304.

Shiau S Y. 1997. Utilization of carbohydrates in warmwater fish-with particular reference to tilapia, Oreochromis niloticus X O. aureus. Aquacul ture, 151 (1-4): 79–96.

Stone D A J, Allan G L, Anderson A J. 2003. Carbohydrate utilization by juvenile silver perch, Bidyanus bidyanus (Mitchell). III. The protein-sparing effect of wheat starchbased carbohydrates. Aquaculture Research, 34 (2): 123–134.

Thivend P, Mercier C, Guilbot A. 1972. Determination of starch with glucoamylase. In: Whistler R L, Bemiller J N eds. Methods in Carbohydrate Chemistry. Academic Press, New York, USA. p.100–105.

Tian L X, Liu Y J, Feng J, Liu D H, Liang G Y, Du Z Y. 2002. Effect of different types of starch on growth, the deposition of mesenteric fat and body composition of Ctenopharyngodon idellus. Journal of Fisheries of China, 26 (3): 247–251. (in Chinese with English abstract)

Tranulis M A, Christophersen B, Blom A K, Borrebaek B. 1991. Glucose dehydrogenase, glucose-6-phosphate dehydrogenase and hexokinase in liver of rainbow trout (Salmo gairdneri). Effects of starvation and temperature variations. Comparative Biochemistry and Physiology Part B: Comparative Biochemistry, 99 (3): 687–691.

浏览量

Downloads

CSCD

文章被引用时，请邮件提醒。

Submit

工具集

关联资源

Combined proteomic and metabolomic studies on the liver of Amur sturgeon Acipenserschrenckii under titanium dioxide nanoparticle exposure

Impacts of dietary supplementation of bamboo vinegar and charcoal powder on growth performance, intestinal morphology, and gut microflora of large-scale loach Paramisgurnus dabryanus

Growth performance, digestive enzyme activities and serum nonspecific immunity of the red tilapia (Oreochromis mossambicus×Oreochromis niloticus) fed diets supplemented with ultrafine powder of Enteromopha prolifera

Dietary effects of Azolla pinnata combined with exogenous digestive enzyme (Digestin^TM) on growth and nutrients utilization of freshwater prawn, Macrobrachium rosenbergii (de Man 1879)

Metyrapone supplementation mitigates cortisol-mediated growth inhibition and sex bias in high-density cultured Japanese eels Anguilla japonica