Polymorphisms of clip domain serine proteinase and serine proteinase homolog in the swimming crab Portunus trituberculatus and their association with Vibrio alginolyticus

Meng Liu; Yuan Liu; Min Hui; Chengwen Song; Zhaoxia Cui

doi:10.1007/s00343-016-5331-7

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Polymorphisms of clip domain serine proteinase and serine proteinase homolog in the swimming crab Portunus trituberculatus and their association with Vibrio alginolyticus

OriginalPaper | Updated：2023-05-16

- Polymorphisms of clip domain serine proteinase and serine proteinase homolog in the swimming crab Portunus trituberculatus and their association with Vibrio alginolyticus
- Polymorphisms of clip domain serine proteinase and serine proteinase homolog in the swimming crab Portunus trituberculatus and their association with Vibrio alginolyticus
- 海洋湖沼学报（英文） 2017年35卷第2期页码：235-243
- Affiliations：
  
  1. College of Fisheries and Life Science Dalian Ocean University
  2. Key Laboratory of Experimental Marine Biology Institute of Oceanology Chinese Academy of Sciences
  3. Laboratory for Marine Biology and Biotechnology Qingdao National Laboratory for Marine Science and Technology
  4. National & Local Joint Engineering Laboratory of Ecological Mariculture
- Author bio：
- Funds：
  
  Supported by the National Natural Science Foundation of China (Nos. 41206147, 31302187) and the Scientific and Technological Innovation Project Financially Supported by Qingdao National Laboratory for Marine Science and Technology (No. 2015ASKJ02)
- DOI：10.1007/s00343-016-5331-7
  中图分类号：
- 网络首发：2016-05-26，
  
  纸质出版：2017
- Accepted：
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Polymorphisms of clip domain serine proteinase and serine proteinase homolog in the swimming crab Portunus trituberculatus and their association with Vibrio alginolyticus[J]. 海洋湖沼学报（英文）, 2017,35(2):235-243.

Liu, M., Liu, Y., Hui, M. et al. Polymorphisms of clip domain serine proteinase and serine proteinase homolog in the swimming crab Portunus trituberculatus and their association with Vibrio alginolyticus, ., Chin. J. Ocean. Limnol. 35, 235–243 (2017). https://doi.org/10.1007/s00343-016-5331-7
Polymorphisms of clip domain serine proteinase and serine proteinase homolog in the swimming crab Portunus trituberculatus and their association with Vibrio alginolyticus[J]. 海洋湖沼学报（英文）, 2017,35(2):235-243. DOI： 10.1007/s00343-016-5331-7.

Liu, M., Liu, Y., Hui, M. et al. Polymorphisms of clip domain serine proteinase and serine proteinase homolog in the swimming crab Portunus trituberculatus and their association with Vibrio alginolyticus, ., Chin. J. Ocean. Limnol. 35, 235–243 (2017). https://doi.org/10.1007/s00343-016-5331-7 DOI：

摘要

Abstract

Clip domain serine proteases (cSPs) and their homologs (SPHs) play an important role in various biological processes that are essential components of extracellular signaling cascades

especially in the innate immune responses of invertebrates. Here

polymorphisms of PtcSP and PtSPH from the swimming crab Portunus trituberculatus were investigated to explore their association with resistance/susceptibility to Vibrio alginolyticus. Polymorphic loci were identified using Clustal X

and characterized with SPSS 16.0 software

and then the significance of genotype and allele frequencies between resistant and susceptible stocks was determined by a χ 2 test. A total of 109 and 77 single nucleotide polymorphisms (SNPs) were identified in the genomic fragments of PtcSP and PtSPH

respectively. Notably

nearly half of PtSPH polymorphisms were found in the non-coding exon 1. Fourteen SNPs investigated were significantly associated with susceptibility/resistance to V. alginolyticus (P <0.05). Among them

eight SNPs were observed in introns

and one synonymous

four non-synonymous SNPs and one ins-del were found in coding exons. In addition

five simple sequence repeats (SSRs) were detected in intron 3 of PtcSP. Although there was no statistically significant difference of allele frequencies

the SSRs showed different polymorphic alleles on the basis of the repeat number between resistant and susceptible stocks. After further validation

polymorphisms investigated here might be applied to select potential molecular markers of P. trituberculatus with resistance to V. alginolyticus.

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references

Bao Y B, Li L, Zhang G F. 2010. Polymorphism of the superoxide dismutase gene family in the bay scallop (Argopecten irradians) and its association with resistance/ susceptibility to Vibrio anguillarum. Developmental & Comparative Immunology, 34 (5): 553–561.

Chen J M, Sun Y X. 2011. Variation in the analysis of positively selected sites using nonsynonymous/synonymous rate ratios: an example using influenza virus. PLoS One, 6 (5): e19996.

Cui Z X, Liu Y, Wu D H, Luan W S, Wang S Y, Li Q Q, Song C W. 2010. Molecular cloning and characterization of a serine proteinase homolog prophenoloxidase-activating factor in the swimming crab Portunus trituberculatus. Fish & Shellfish Immunology, 29 (4): 679–686.

Dekkers J C M, Hospital F. 2002. The use of molecular genetics in the improvement of agricultural populations. Nature Reviews Genetics, 3 (1): 22–32.

Duan J B, Wainwright M S, Comeron J M, Saitou N, Sanders A R, Gelernter J, Gejman P V. 2003. Synonymous mutations in the human dopamine receptor D2 (DRD2) affect mRNA stability and synthesis of the receptor. Human Molecular Genetics, 12 (3): 205–216.

Hancock J M. 1995. The contribution of slippage-like processes to genome evolution. Journal of Molecular Evolution, 41 (6): 1038–1047.

Hao G J, Lin F, Mu C K, Li R H, Yao J Y, Yuan X M, Pan X Y, Shen J Y, Wang C L. 2015. SNP E4-205 C/T in C-type lectin of Portunus trituberculatus is association with susceptibility/resistance to Vibrio alginolyticus challenge. Aquaculture, 442: 125–131.

Hershberg R, Petrov D A. 2008. Selection on codon bias. Annual Review of Genetics, 42 (1): 287–299.

Li L, Zhao J M, Wang L L, Qiu L M, Zhang H, Dong C H, Cong M, Song L S. 2009. The polymorphism of lysozyme gene in Zhikong scallop (Chlamys farreri) and its association with susceptibility/resistance to Listonella anguillarum. Fish & Shellfish Immunology, 27 (2): 136–142.

Li X H, Cui Z X, Liu Y, Song C W, Shi G H, Wang C L. 2013. Polymorphisms of anti-lipopolysaccharide factors in the swimming crab Portunus trituberculatus and their association with resistance/susceptibility to Vibrio alginolyticus. Fish & Shellfish Immunology, 34 (6): 1560–1568.

Liu Q, Wang X Z, Dai F Y, Liu P, Li J. 2007. Preliminary study on Vibrio alginolyticus disease in Portunus trituberculatus. Shandong Fish, 24 (9): 1–3. (in Chinese with English abstract)

Liu Z J, Cordes J F. 2004. DNA marker technologies and their applications in aquaculture genetics. Aquaculture, 238 (1-4): 1–37.

Liu Z J. 2007. Single nucleotide polymorphism (SNP). In: Liu Z J ed. Aquaculture Genome Technologies. Blackwell, USA. p.59–72.

Pagani F, Baralle F E. 2004. Genomic variants in exons and introns: identifying the splicing spoilers. Nature Reviews Genetics, 5 (5): 389–396.

Song C W, Cui Z X, Liu Y, Li Q Q, Li X H, Shi G H, Wang C L. 2013a. Characterization and functional analysis of serine proteinase and serine proteinase homologue from the swimming crab Portunus trituberculatus. Fish & Shellfish Immunology, 35 (2): 231–239.

Song C W, Luo D L, Cui Z X, Liu Y, Li X H, Shi G H, Wang C L. 2013b. Polymorphism of crustins in the swimming crab (Portunus trituberculatus) and its association with Vibrio alginolyticus. Aquaculture Research, 46 (5): 1261–1268.

Thanh N M, Barnes A C, Mather P B, Li Y T, Lyons R E. 2010. Single nucleotide polymorphisms in the actin and crustacean hyperglycemic hormone genes and their correlation with individual growth performance in giant freshwater prawn Macrobrachium rosenbergii. Aquaculture, 301 (1-4): 7–15.

Vignal A, Milan D, San Cristobal M, Eggen A. 2002. A review on SNP and other types of molecular markers and their use in animal genetics. Genetics Selection Evolution, 34: 275.

Wan X H, Shen H, Wang L B, Cheng Y X. 2011. Isolation and characterization of Vibrio metschnikovii causing infection in farmed Portunus trituberculatus in China. Aquaculture International, 19 (2): 351–359.

Wang G L, Jin S, Chen Y E, Li Z. 2006. Study on pathogens and pathogenesis of emulsification disease of Portunus trituberculatus. Advances in Marine Science, 24 (4): 526–531. (in Chinese with English abstract)

Wang L, Su J G, Yang C R, Wan Q Y, Peng L M. 2012. Genomic organization, promoter activity of grass carp MDA5 and the association of its polymorphisms with susceptibility/ resistance to grass carp reovirus. Mol ecular Immunol ogy, 50 (4): 236–243.

Yue X, Wang H X, Huang X H, Wang C, Chai X L, Wang C D, Liu B Z. 2012. Single nucleotide polymorphisms in i-type lysozyme gene and their correlation with vibrio-resistance and growth of clam Meretrix meretrix based on the selected resistance stocks. Fish & Shellfish Immunology, 33 (3): 559–568.

Zeng D G, Chen X H, Li Y M et al. 2008. Analysis of Hsp70 in Litopenaeus vannamei and detection of SNPs. Journal of Crustacean Biology, 28 (4): 727–730.

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