Survival, recovery and microcystin release of Microcystis aeruginosa in cold or dark condition

Yi Ding; Nanqin Gan; Jin Liu; Lingling Zheng; Lin Li; Lirong Song

doi:10.1007/s00343-016-5215-x

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Survival, recovery and microcystin release of Microcystis aeruginosa in cold or dark condition

OriginalPaper | Updated：2023-05-16

- Survival, recovery and microcystin release of Microcystis aeruginosa in cold or dark condition
- Survival, recovery and microcystin release of Microcystis aeruginosa in cold or dark condition
- 海洋湖沼学报（英文） 2017年35卷第2期页码：313-323
- Affiliations：
  
  1. State Key Laboratory of Freshwater Ecology and Biotechnology Institute of Hydrobiology Chinese Academy of Sciences
  2. Key Laboratory of Plant Germplasm Enhancement and Speciality Agriculture Wuhan Botanical Garden Chinese Academy of Sciences
- Author bio：
- Funds：
  
  Supported by the National Natural Science Foundation of China (Nos. 31070355, 31370418)
- DOI：10.1007/s00343-016-5215-x
  中图分类号：
- 网络首发：2016-05-10，
  
  纸质出版：2017
- Accepted：
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Survival, recovery and microcystin release of Microcystis aeruginosa in cold or dark condition[J]. 海洋湖沼学报（英文）, 2017,35(2):313-323.

Ding, Y., Gan, N., Liu, J. et al. Survival, recovery and microcystin release of Microcystis aeruginosa in cold or dark condition., Chin. J. Ocean. Limnol. 35, 313–323 (2017). https://doi.org/10.1007/s00343-016-5215-x
Survival, recovery and microcystin release of Microcystis aeruginosa in cold or dark condition[J]. 海洋湖沼学报（英文）, 2017,35(2):313-323. DOI： 10.1007/s00343-016-5215-x.

Ding, Y., Gan, N., Liu, J. et al. Survival, recovery and microcystin release of Microcystis aeruginosa in cold or dark condition., Chin. J. Ocean. Limnol. 35, 313–323 (2017). https://doi.org/10.1007/s00343-016-5215-x DOI：

摘要

Abstract

Microcystis often dominates phytoplankton in eutrophic lakes and must survive a long period of cold or dark conditions. However

the survival strategies of Microcystis to withstand cold or dark stress are less well known. In this study

we conducted experiments on the responses of two toxic Microcystis aeruginosa strains (FACHB-905 and FACHB-915) and their microcystin release in conditions of low temperature (15°C or 4°C

with illumination) or darkness

and subsequent recovery in standard conditions (25°C with illumination). On exposure to 15°C

a small decrease in cell viability was observed

but the cell number increased gradually

suggesting that M. aeruginosa FACHB-905 and FACHB-915 cells seem in general tolerant in 15°C. Interestingly

our results show that a higher carotenoid content and microcystin release potentially enhance the fitness of surviving cells at 15°C. M. aeruginosa cells exposed to lower temperature light stress (4°C) did not completely lose viability and retained the ability to reinitiate growth. In darkness

the maximum quantum yield (F v/F m) and the maximum electron transport rate (ETRmax) values and cell viability of M. aeruginosa cells gradually decreased with time. During the recovery period

the photosynthetic efficiency of M. aeruginosa reverted to the normal level. Additionally

M. aeruginosa FACHB-905 and FACHB-915 exposed to low temperature had increased caspase-3-like activity and DNA fragmentation

which suggests the occurrence of a type of cell death in M. aeruginosa cells under cold stress similar to programmed cell death. Overall

our findings could confer certain advantages on the Microcystis for surviving cold or dark conditions encountered in the annual cycle

and help explain its repeated occurrence in water blooms in large and shallow lakes.

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references

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