The combined effects of Dolichospermum flos-aquae, light, and temperature on microcystin production by Microcystis aeruginosa

Ruoqi Chen; Fangfang Li; Jiadong Liu; Hongye Zheng; Fei Shen; Yarong Xue; Changhong Liu

doi:10.1007/s00343-016-5204-0

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The combined effects of Dolichospermum flos-aquae, light, and temperature on microcystin production by Microcystis aeruginosa

OriginalPaper | Updated：2023-05-16

- The combined effects of Dolichospermum flos-aquae, light, and temperature on microcystin production by Microcystis aeruginosa
- The combined effects of Dolichospermum flos-aquae, light, and temperature on microcystin production by Microcystis aeruginosa
- 海洋湖沼学报（英文） 2016年34卷第6期页码：1173-1182
- Affiliations：
  
  State Key Laboratory of Pharmaceutical Biotechnology School of Life Science Nanjing University
- Author bio：
- Funds：
  
  Supported by the National Natural Science Foundation of China (Nos. 31471810, 31272081), the Major Science and Technology Program for Water Pollution Control and Treatment (No. 2012ZX07101-013-05(02)), and the Jiangsu Key Technology R&D Program (No. BE2012372)
- DOI：10.1007/s00343-016-5204-0
  中图分类号：
- 网络首发：2016-03-14，
  
  纸质出版：2016
- Accepted：
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The combined effects of Dolichospermum flos-aquae, light, and temperature on microcystin production by Microcystis aeruginosa[J]. 海洋湖沼学报（英文）, 2016,34(6):1173-1182.

Chen, R., Li, F., Liu, J. et al. The combined effects of Dolichospermum flos-aquae, light, and temperature on microcystin production by Microcystis aeruginosa, ., Chin. J. Ocean. Limnol. 34, 1173–1182 (2016). https://doi.org/10.1007/s00343-016-5204-0
The combined effects of Dolichospermum flos-aquae, light, and temperature on microcystin production by Microcystis aeruginosa[J]. 海洋湖沼学报（英文）, 2016,34(6):1173-1182. DOI： 10.1007/s00343-016-5204-0.

Chen, R., Li, F., Liu, J. et al. The combined effects of Dolichospermum flos-aquae, light, and temperature on microcystin production by Microcystis aeruginosa, ., Chin. J. Ocean. Limnol. 34, 1173–1182 (2016). https://doi.org/10.1007/s00343-016-5204-0 DOI：

摘要

Abstract

The effects of light

temperature

and coculture on the intracellular microcystin-LR (MC-LR) quota of Microcystis aeruginosa were evaluated based on coculture experiments with nontoxic Dolichospermum (Anabaena) flos-aquae. The MC-LR quota and transcription of mcyB and mcyD genes encoding MC synthetases in M. aeruginosa were evaluated on the basis of cell counts

high-performance liquid chromatography

and reverse-transcription quantitative real-time PCR. The MC-LR quotas of M. aeruginosa in coculture with a 1/1 ratio of inoculum of the two species were significantly lower relative to monocultures 6-d after inoculation. Decreased MC-LR quotas under coculture conditions were enhanced by increasing the D. flos-aquae to M. aeruginosa ratio in the inoculum and by environmental factors

such as temperature and light intensity. Moreover

the transcriptional concentrations of mcyB and mcyD genes in M. aeruginosa were significantly inhibited by D. flos-aquae competition in coculture (P <0.01)

lowered to 20% of initial concentrations within 8 days. These data suggested that coculture eff ects by D. flos-aquae not only reduced M. aeruginosa’s intracellular MC-LR quota via inhibition of genes encoding MC synthetases

but also that this eff ect was regulated by environmental factors

including temperature and light intensities.

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Keywords

references

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