Functional characterization of a novel microbial esterase identified from the Indian Ocean and its use in the stereoselective preparation of (R)-methyl mandelate

Jiayuan Liang; Aijun Sun; Yun Zhang; Dun Deng; Yongfei Wang; Sanmei Ma; Yunfeng Hu

doi:10.1007/s00343-016-5164-4

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Functional characterization of a novel microbial esterase identified from the Indian Ocean and its use in the stereoselective preparation of (R)-methyl mandelate

OriginalPaper | Updated：2023-05-16

- Functional characterization of a novel microbial esterase identified from the Indian Ocean and its use in the stereoselective preparation of (R)-methyl mandelate
- Functional characterization of a novel microbial esterase identified from the Indian Ocean and its use in the stereoselective preparation of (R)-methyl mandelate
- 海洋湖沼学报（英文） 2016年34卷第6期页码：1269-1277
- Affiliations：
  
  1. Guangdong Key Laboratory of Marine Materia Medica South China Sea Institute of Oceanology Chinese Academy of Sciences
  2. Key Laboratory of Tropical Marine Bio-Resources and Ecology South China Sea Institute of Oceanology Chinese Academy of Sciences
  3. Department of Biotechnology Jinan University
  4. South China Sea Bio-Resource Exploitation and Utilization Collaborative Innovation Center
- Author bio：
- Funds：
  
  Supported by the National Natural Science Foundation of China (No. 21302199), the Strategic Priority Research Program of the Chinese Academy of Sciences (No. XDA11030404), the Project of “Engineering High-Performance Microorganisms for Advanced Bio-Based Manufacturing” from the Chinese Academy of Sciences (No. KGZD-EW-606), and the Guangzhou Science and Technology Plan Projects (No. 201510010012)
- DOI：10.1007/s00343-016-5164-4
  中图分类号：
- 网络首发：2016-03-19，
  
  纸质出版：2016
- Accepted：
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Functional characterization of a novel microbial esterase identified from the Indian Ocean and its use in the stereoselective preparation of (R)-methyl mandelate[J]. 海洋湖沼学报（英文）, 2016,34(6):1269-1277.

Liang, J., Sun, A., Zhang, Y. et al. Functional characterization of a novel microbial esterase identified from the Indian Ocean and its use in the stereoselective preparation of (R)-methyl mandelate., Chin. J. Ocean. Limnol. 34, 1269–1277 (2016). https://doi.org/10.1007/s00343-016-5164-4
Functional characterization of a novel microbial esterase identified from the Indian Ocean and its use in the stereoselective preparation of (R)-methyl mandelate[J]. 海洋湖沼学报（英文）, 2016,34(6):1269-1277. DOI： 10.1007/s00343-016-5164-4.

Liang, J., Sun, A., Zhang, Y. et al. Functional characterization of a novel microbial esterase identified from the Indian Ocean and its use in the stereoselective preparation of (R)-methyl mandelate., Chin. J. Ocean. Limnol. 34, 1269–1277 (2016). https://doi.org/10.1007/s00343-016-5164-4 DOI：

摘要

Abstract

Genomic mining has identified a novel microbial alkaline esterase from the Indian Ocean. This esterase was overexpressed in E. coli BL21 (DE3) and further functionally characterized. Under optimal conditions (10 mmol/L substrate

pH 6.0

2 h at 40 °C)

this esterase can hydrolyze racemic methyl mandelate to (R)-methyl mandelate with very high optical purity (e.e. >99%) and yield (nearly 90%). Interestingly

the stereoselectivity of this esterase is opposite to that of two previously reported lipases that can generate (S)-methyl mandelate through the hydrolysis of racemic methyl mandelate. No organic solvents or other additives were required to optimize the optical purity and production of the final chiral product (R)-methyl mandelate

which can potentially simplify the production procedure of (R)-methyl mandelate catalyzed by esterase.

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references

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