Profile of candidate microsatellite markers in Sebastiscus marmoratus using 454 pyrosequencing

Na Song; Muyan Chen; Tianxiang Gao; Takashi Yanagimoto

doi:10.1007/s00343-016-5103-4

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Profile of candidate microsatellite markers in Sebastiscus marmoratus using 454 pyrosequencing

OriginalPaper | Updated：2023-05-16

- Profile of candidate microsatellite markers in Sebastiscus marmoratus using 454 pyrosequencing
- Profile of candidate microsatellite markers in Sebastiscus marmoratus using 454 pyrosequencing
- 海洋湖沼学报（英文） 2017年35卷第1期页码：198-202
- Affiliations：
  
  1. Fisheries College Ocean University of China
  2. Fisheries College Zhejiang Ocean University
  3. National Research Institute of Fisheries Science Fisheries Research Agency
- Author bio：
- Funds：
  
  Supported by the Special Fund for Agro-scientific Research in the Public Interest (201303048), the National Natural Science Foundation of China (Nos. 41176117, 31172447), and the National Infrastructure of Fishery Germplasm Resources;SONG Na and CHEN Muyan contributed equally to this work.;An erratum to this article is available at;http://dx.doi.org/10.1007/s00343-017-7466-6;.
- DOI：10.1007/s00343-016-5103-4
  中图分类号：
- 网络首发：2016-04-21，
  
  纸质出版：2017
- Accepted：
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Profile of candidate microsatellite markers in Sebastiscus marmoratus using 454 pyrosequencing[J]. 海洋湖沼学报（英文）, 2017,35(1):198-202.

Song, N., Chen, M., Gao, T. et al. Profile of candidate microsatellite markers in Sebastiscus marmoratus using 454 pyrosequencing., Chin. J. Ocean. Limnol. 35, 198–202 (2017). https://doi.org/10.1007/s00343-016-5103-4
Profile of candidate microsatellite markers in Sebastiscus marmoratus using 454 pyrosequencing[J]. 海洋湖沼学报（英文）, 2017,35(1):198-202. DOI： 10.1007/s00343-016-5103-4.

Song, N., Chen, M., Gao, T. et al. Profile of candidate microsatellite markers in Sebastiscus marmoratus using 454 pyrosequencing., Chin. J. Ocean. Limnol. 35, 198–202 (2017). https://doi.org/10.1007/s00343-016-5103-4 DOI：

摘要

Abstract

Sebastiscus marmoratus is an important sedentary ovoviparous fish distributed in near-shore coastal waters from the coast of China to Japan. Candidate S. marmoratus microsatellite markers were developed in the present study using 454 pyrosequencing

and the marker profile was analyzed. A total of 2 000 000 raw sequence reads were assembled to reduce redundancy. Among them

1 043 dinucleotide

925 trinucleotide

692 tetranucleotide

and 315 pentanucleotide repeats were detected. AC repeats were the most frequent motifs among the dinucleotide repeats

and AAT was the most abundant among the trinucleotide repeats. AAAT

ATAG

and ATCC were the three most common tetranucleotide motifs

and AAGAT and AATAT were the most dominant pentanucleotide motifs. The greatest numbers of loci and potentially amplifiable loci were found in dinucleotide repeats

whereas trinucleotide repeats had the fewest. In summary

a wide range of candidate microsatellite markers were identified in the present study using a rapid and efficient 454 pyrosequencing approach.

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references

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