Effects of chilled storage and cryopreservation on sperm characteristics, antioxidant enzyme activities, and lipid peroxidation in Pacific cod Gadus microcephalus

Xueying Wang; Xuehui Shi; Yifan Liu; Daode Yu; Shuguang Guan; Qinghua Liu; Jun Li

doi:10.1007/s00343-016-5088-z

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Effects of chilled storage and cryopreservation on sperm characteristics, antioxidant enzyme activities, and lipid peroxidation in Pacific cod Gadus microcephalus

OriginalPaper | Updated：2023-05-16

- Effects of chilled storage and cryopreservation on sperm characteristics, antioxidant enzyme activities, and lipid peroxidation in Pacific cod Gadus microcephalus
- Effects of chilled storage and cryopreservation on sperm characteristics, antioxidant enzyme activities, and lipid peroxidation in Pacific cod Gadus microcephalus
- 海洋湖沼学报（英文） 2016年34卷第4期页码：763-771
- Affiliations：
  
  1. Key Laboratory of Experimental Marine Biology Institute of Oceanology Chinese Academy of Sciences
  2. College of Earth Science University of Chinese Academy of Sciences
  3. College of Chemistry and Pharmaceutical Sciences Qingdao Agricultural University
  4. Marine Biology Institute of Shandong Province
  5. National & Local Joint Engineering Laboratory of Ecological Mariculture Chinese Academy of Sciences
- Author bio：
- Funds：
  
  Supported by the National Natural Science Foundation of China (Nos. 31072212, 41076100), the CAS Scientific and Technological Innovation Program for Cross and Cooperative Team, Marine Economy Innovative Development Project (No. 12PYY001SF08), and the National Key Basic Program of Science and Technology Platforms of Aquaculture Stock Resources, Shandong Technology Development Project (No. 2013GHY11509)
- DOI：10.1007/s00343-016-5088-z
  中图分类号：
- 网络首发：2016-01-07，
  
  纸质出版：2016
- Accepted：
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Effects of chilled storage and cryopreservation on sperm characteristics, antioxidant enzyme activities, and lipid peroxidation in Pacific cod Gadus microcephalus[J]. 海洋湖沼学报（英文）, 2016,34(4):763-771.

Wang, X., Shi, X., Liu, Y. et al. Effects of chilled storage and cryopreservation on sperm characteristics, antioxidant enzyme activities, and lipid peroxidation in Pacific cod Gadus microcephalus, ., Chin. J. Ocean. Limnol. 34, 763–771 (2016). https://doi.org/10.1007/s00343-016-5088-z
Effects of chilled storage and cryopreservation on sperm characteristics, antioxidant enzyme activities, and lipid peroxidation in Pacific cod Gadus microcephalus[J]. 海洋湖沼学报（英文）, 2016,34(4):763-771. DOI： 10.1007/s00343-016-5088-z.

Wang, X., Shi, X., Liu, Y. et al. Effects of chilled storage and cryopreservation on sperm characteristics, antioxidant enzyme activities, and lipid peroxidation in Pacific cod Gadus microcephalus, ., Chin. J. Ocean. Limnol. 34, 763–771 (2016). https://doi.org/10.1007/s00343-016-5088-z DOI：

摘要

Abstract

The present study evaluated the effects of chilled storage and cryopreservation on sperm motion characteristics

antioxidant enzyme activities

and lipid peroxidation in the Pacific cod Gadus macrocephalus. Sperm motility and the activities of superoxide dismutase (SOD)

catalase (CAT)

glutathione peroxidase (GPx)

glutathione reductase (Gr)

and lipid peroxidation (measured via malondialdehyde (MDA) content) were determined after the milt was stored at 4°C for 12 h

cryopreserved without cryoprotectant in 12% propylene glycol (PG)

cryopreserved in 12% PG+0.1 mol/L trehalose

or cryopreserved in 12% PG spermatozoa but centrifuged to decant the supernatant prior to cryopreservation (only sperm cells were cryopreserved). After chilled storage or cryopreservation

the SOD

CAT and GPx activities were reduced in sperm cells and increased in seminal plasma in almost all treatments; sperm motility parameters were also decreased. However

the addition of trehalose into the cryoprotectant could significantly improve the postthaw sperm quality as revealed by the sperm average path velocity. This improvement might be attributed to the function of trehalose in scavenging reactive oxygen species. Chilled storage and cryopreservation had significant effects on sperm motion characteristics

antioxidant enzyme activities

and lipid peroxidation in the Pacific cod.

关键词

Keywords

references

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