The metabolite profiling of coastal coccolithophorid species Pleurochrysis carterae (Haptophyta)

Chengxu Zhou; Jie Luo; Yangfang Ye; Xiaojun Yan; Baoning Liu; Xin Wen

doi:10.1007/s00343-016-5042-0

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The metabolite profiling of coastal coccolithophorid species Pleurochrysis carterae (Haptophyta)

OriginalPaper | Updated：2023-05-16

- The metabolite profiling of coastal coccolithophorid species Pleurochrysis carterae (Haptophyta)
- The metabolite profiling of coastal coccolithophorid species Pleurochrysis carterae (Haptophyta)
- 海洋湖沼学报（英文） 2016年34卷第4期页码：749-756
- Affiliations：
  
  School of Marine Sciences Ningbo University
- Author bio：
- Funds：
- DOI：10.1007/s00343-016-5042-0
  中图分类号：
- 网络首发：2015-11-24，
  
  纸质出版：2016
- Accepted：
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The metabolite profiling of coastal coccolithophorid species Pleurochrysis carterae (Haptophyta)[J]. 海洋湖沼学报（英文）, 2016,34(4):749-756.

Zhou, C., Luo, J., Ye, Y. et al. The metabolite profiling of coastal coccolithophorid species Pleurochrysis carterae (Haptophyta)., Chin. J. Ocean. Limnol. 34, 749–756 (2016). https://doi.org/10.1007/s00343-016-5042-0
The metabolite profiling of coastal coccolithophorid species Pleurochrysis carterae (Haptophyta)[J]. 海洋湖沼学报（英文）, 2016,34(4):749-756. DOI： 10.1007/s00343-016-5042-0.

Zhou, C., Luo, J., Ye, Y. et al. The metabolite profiling of coastal coccolithophorid species Pleurochrysis carterae (Haptophyta)., Chin. J. Ocean. Limnol. 34, 749–756 (2016). https://doi.org/10.1007/s00343-016-5042-0 DOI：

摘要

Abstract

Pleurochrysis carterae is a calcified coccolithophorid species that usually blooms in the coastal area and causes aquaculture losses. The cellular calcification

blooming and many other critical species specific eco-physiological processes are closely related to various metabolic pathways. The purpose of this study is to apply the unbiased and non-destructive method of nuclear magnetic resonance (NMR) to detect the unknown holistic metabolite of P. carterae. The results show that NMR spectroscopic method is practical in the analysis of metabolites of phytoplankton. The metabolome of P. carterae was dominated by 26 metabolites involved in a number of different primary and secondary metabolic pathways. Organic acids and their derivatives

amino acids

sugars

nucleic aides were mainly detected. The abundant metabolites are that closely related to the process of cellular osmotic adjustment

which possibly reflect the active ability of P. carterae to adapt to the versatile coastal niche. DMSP (dimethylsulphoniopropionate) was the most dominant metabolite in P. carterae

up to 2.065±0.278 mg/g lyophilized cells

followed by glutamate and lactose

the contents were 0.349±0.035 and 0.301±0.073 mg/g lyophilized cells respectively. Other metabolites that had the content ranged between 0.1–0.2 mg/g lyophilized cells were alanine

isethionate and arabinose. Amino acid (valine

phenylalanine

isoleucine

tyrosine)

organic acid salts (lactate

succinate)

scyllitol and uracil had content ranged from 0.01 to below 0.1 mg/g lyophilized cells. Trigonelline

fumarate and formate were detected in very low content (only thousandths of 1 mg per gram of lyophilized cells or below). Our results of the holistic metabolites of P. carterae are the basic references for the further studies when multiple problems will be addressed to this notorious blooming calcifying species.

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references

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