Complete nuclear ribosomal DNA sequence amplification and molecular analyses of Bangia (Bangiales, Rhodophyta) from China

Jiajie Xu; Bo Jiang; Sanming Chai; Yuan He; Jianyi Zhu; Zonggen Shen; Songdong Shen

doi:10.1007/s00343-016-5033-1

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Complete nuclear ribosomal DNA sequence amplification and molecular analyses of Bangia (Bangiales, Rhodophyta) from China

OriginalPaper | Updated：2023-05-16

- Complete nuclear ribosomal DNA sequence amplification and molecular analyses of Bangia (Bangiales, Rhodophyta) from China
- Complete nuclear ribosomal DNA sequence amplification and molecular analyses of Bangia (Bangiales, Rhodophyta) from China
- 海洋湖沼学报（英文） 2016年34卷第5期页码：1044-1053
- Affiliations：
  
  1. Department of Cell Biology College of Biology and Basic Medical Sciences Soochow University SIP
  2. Department of Biology and Food Engineering Changshu Institute of Technology
  3. Maternal and Child Health Hospital of Gansu Province
- Author bio：
- Funds：
  
  Supported by the National Natural Science Foundation of China (Nos. 31270256, 41276134) and the National High Technology Research and Development Program of China (863 Program) (No. 2012AA10A406-6);XU Jiajie and CHAI Sanming contributed equally to this work.
- DOI：10.1007/s00343-016-5033-1
  中图分类号：
- 网络首发：2016-01-07，
  
  纸质出版：2016
- Accepted：
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Complete nuclear ribosomal DNA sequence amplification and molecular analyses of Bangia (Bangiales, Rhodophyta) from China[J]. 海洋湖沼学报（英文）, 2016,34(5):1044-1053.

Xu, J., Jiang, B., Chai, S. et al. Complete nuclear ribosomal DNA sequence amplification and molecular analyses of Bangia (Bangiales, Rhodophyta) from China., Chin. J. Ocean. Limnol. 34, 1044–1053 (2016). https://doi.org/10.1007/s00343-016-5033-1
Complete nuclear ribosomal DNA sequence amplification and molecular analyses of Bangia (Bangiales, Rhodophyta) from China[J]. 海洋湖沼学报（英文）, 2016,34(5):1044-1053. DOI： 10.1007/s00343-016-5033-1.

Xu, J., Jiang, B., Chai, S. et al. Complete nuclear ribosomal DNA sequence amplification and molecular analyses of Bangia (Bangiales, Rhodophyta) from China., Chin. J. Ocean. Limnol. 34, 1044–1053 (2016). https://doi.org/10.1007/s00343-016-5033-1 DOI：

摘要

Abstract

Filamentous Bangia

which are distributed extensively throughout the world

have simple and similar morphological characteristics. Scientists can classify these organisms using molecular markers in combination with morphology. We successfully sequenced the complete nuclear ribosomal DNA

approximately 13 kb in length

from a marine Bangia population. We further analyzed the small subunit ribosomal DNA gene (nrSSU) and the internal transcribed spacer (ITS) sequence regions along with nine other marine

and two freshwater Bangia samples from China. Pairwise distances of the nrSSU and 5.8S ribosomal DNA gene sequences show the marine samples grouping together with low divergences (00.003; 0–0.006

respectively) from each other

but high divergences (0.123–0.126; 0.198

respectively) from freshwater samples. An exception is the marine sample collected from Weihai

which shows high divergence from both other marine samples (0.063–0.065; 0.129

respectively) and the freshwater samples (0.097; 0.120

respectively). A maximum likelihood phylogenetic tree based on a combined SSU-ITS dataset with maximum likelihood method shows the samples divided into three clades

with the two marine sample clades containing Bangia spp. from North America

Europe

Asia

and Australia; and one freshwater clade

containing Bangia atropurpurea from North America and China.

关键词

Keywords

references

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