Characterization and in-vivo evaluation of potential probiotics of the bacterial flora within the water column of a healthy shrimp larviculture system

Ming Xue; Huafang Liang; Yaoyao He; Chongqing Wen

doi:10.1007/s00343-016-5024-2

Your Location：

Home >

Browse articles >

Characterization and in-vivo evaluation of potential probiotics of the bacterial flora within the water column of a healthy shrimp larviculture system

OriginalPaper | Updated：2023-05-16

- Characterization and in-vivo evaluation of potential probiotics of the bacterial flora within the water column of a healthy shrimp larviculture system
- Characterization and in-vivo evaluation of potential probiotics of the bacterial flora within the water column of a healthy shrimp larviculture system
- 海洋湖沼学报（英文） 2016年34卷第3期页码：484-491
- Affiliations：
  
  Fisheries College Guangdong Ocean University
- Author bio：
- Funds：
  
  Supported by the National Natural Science Foundation of China (No. 31372536), the Special Projects of Science and Technology Promotion for Marine Fisheries in Guangdong Province (No. A201008B03), and the Startup Programs for Introduction Personnel of Guangdong Ocean University (Nos. E10046, E11328)
- DOI：10.1007/s00343-016-5024-2
  中图分类号：
- 网络首发：2015-11-24，
  
  纸质出版：2016
- Accepted：
Scan QR Code
Characterization and in-vivo evaluation of potential probiotics of the bacterial flora within the water column of a healthy shrimp larviculture system[J]. 海洋湖沼学报（英文）, 2016,34(3):484-491.

Xue, M., Liang, H., He, Y. et al. Characterization and in-vivo evaluation of potential probiotics of the bacterial flora within the water column of a healthy shrimp larviculture system., Chin. J. Ocean. Limnol. 34, 484–491 (2016). https://doi.org/10.1007/s00343-016-5024-2
Characterization and in-vivo evaluation of potential probiotics of the bacterial flora within the water column of a healthy shrimp larviculture system[J]. 海洋湖沼学报（英文）, 2016,34(3):484-491. DOI： 10.1007/s00343-016-5024-2.

Xue, M., Liang, H., He, Y. et al. Characterization and in-vivo evaluation of potential probiotics of the bacterial flora within the water column of a healthy shrimp larviculture system., Chin. J. Ocean. Limnol. 34, 484–491 (2016). https://doi.org/10.1007/s00343-016-5024-2 DOI：

摘要

Abstract

A thorough understanding of the normal bacterial flora associated with shrimp larviculture systems contributes to probiotic screening and disease control. The bacterial community of the water column over a commercial Litopenaeus vannamei larval rearing run was characterized with both culture-dependent and culture-independent methods. A total of 27 phylotypes at the species level were isolated and identified based on 16S rDNA sequence analysis. Denaturing gradient gel electrophoresis (DGGE) analysis of the V3–V5 region of 16S rRNA genes showed a dynamic bacterial community with major changes occurred from stages zoea to mysis during the rearing run. The sequences retrieved were affiliated to four phyla

Proteobacteria

Actinobacteria

Bacteroidetes

and Firmicutes

with the family Rhodobacteraceae being the most frequently recovered one. Subsequently

13 representative strains conferred higher larval survival than the control when evaluated in the in-vivo experiments; in particular

three candidates

assigned to Phaeobacter sp.

Arthrobacter sp.

and Microbacterium sp.

significantly improved larval survival (P < 0.05). Therefore

the healthy shrimp larviculture system harbored a diverse and favorable bacterial flora

which contribute to larval development and are of great importance in exploiting novel probiotics.

关键词

Keywords

references

Beardsley C, Moss S, Malfatti F, Azam F. 2011. Quantitative role of shrimp fecal bacteria in organic matter fluxes in a recirculating shrimp aquaculture system. FEMS Microbiol. Ecol., 77 (1): 134–145.

Blancheton J P, Attramadal K J K, Michaud L, Roque d'Orbcastel E, Vadstein O. 2013. Insight into bacterial population in aquaculture systems and its implication. Aquacult. Eng., 53: 30–39.

D’Alvise P W, Lillebø S, Prol-Garcia M J, Wergeland H I, Nielsen K F, Bergh Ø, Gram L. 2012. Phaeobacter gallaeciensis reduces Vibrio anguillarum in cultures of microalgae and rotifers, and prevents vibriosis in cod larvae. PLoS One, 7 (8): e43996.

Das S, Ward L R, Burke C. 2008. Prospects of using marine actinobacteria as probiotics in aquaculture. Appl. Microbiol. Biotechnol., 81 (3): 419–429.

FAO. 2003. Health management and biosecurity maintenance in white shrimp (Penaeus vannamei) hatcheries in Latin America. FAO Fisheries Technical, Paper No.450. Rome.

Fu H L, Wei Y F, Zou Y Y, Li M Z, Wang F Y, Chen J R, Zhang L X, Liu Z H, Ding L X. 2014. Research progress on the Actinomyces arthrobacter. Adv. Microbiol., 4 (12): 747–753.

Garcia A T, Olmos J S. 2007. Quantification by fluorescent in situ hybridization of bacteria associated with Litopenaeus vannamei larvae in Mexican shrimp hatchery. Aquaculture, 262 (2-4): 211–218.

George M R, John K R, Iyappan T, Jeyaseelan M J P. 2005. Genetic heterogeneity among Vibrio alginolyticus isolated from shrimp farms by PCR fingerprinting. Lett. Appl. Microbiol., 40 (5): 369–372.

Holmström C, Egan S, Franks A, McCloy S, Kjelleberg S. 2002. Anti-fouling activities expressed by marine surface associated Pseudoalteromonas species. FEMS Microbiol. Ecol., 41 (1): 47–58.

Huang Z B, Li X Y, Wang L P, Shao Z Z. 2014. Changes in the intestinal bacterial community during the growth of white shrimp, Litopenaeus vannamei. Aquacult. Res., http://dx. doi.org/10.1111/are.12628.

Jiang Y, Marang L, Kleerebezem R, Muyzer G, Van Loosdrecht M C M. 2011. Effect of temperature and cycle length on microbial competition in PHB-producing sequencing batch reactor. ISME J., 5 (5): 896–907.

Kim O S, Cho Y J, Lee K, Yoon S H, Kim M, Na H, Park S C, Jeon Y S, Lee J H, Yi H N, Won S, Chun J. 2012. Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int. J. Syst. Evol. Microbiol., 62 (3): 716–721.

Li J Q, Tan B P, Mai K S, Ai Q H, Zhang W B, Liufu Z G, Xu W. 2008. Immune responses and resistance against Vibrio parahaemolyticus induced by probiotic bacterium Arthrobacter XE-7 in Pacific white shrimp, Litopenaeus vannamei. J. World Aquacult. Soc., 39 (4): 477–489.

Longeon A, Peduzzi J, Barthélemy M, Corre S, Nicolas J-L, Guyot M. 2004. Purification and partial identification of novel antimicrobial protein from marine bacterium Pseudoalteromonas species strain X153. Mar. Biotechnol., 6 (6): 633–641.

Pangastuti A, Suwanto A, Lestari Y, Suhartono M T. 2010. Bacterial communities associated with white shrimp (Litopenaeus vannamei) larvae at early developmental stages. Biodiversitas, 11 (2): 65–68.

Prado S, Montes J, Romalde J L, Barja J L. 2009. Inhibitory activity of Phaeobacter strains against aquaculture pathogenic bacteria. Int. Microbiol., 12 (2): 107–114.

Ruiz-Ponte C, Samain J F, Sánchez J L, Nicolas J L. 1999. The benefit of a Roseobacter species on the survival of scallop larvae. Mar. Biotechnol., 1 (1): 52–59.

Sakami T, Koiso M, Sugaya T. 2014. Characterization of bacterial community composition in rotifer cultures under unexpected growth suppression. Fish Sci., 80 (4): 757–765.

Schulze A D, Alabi A O, Tattersall-Sheldrake A R, Miller K M. 2006. Bacterial diversity in a marine hatchery: balance between pathogenic and potentially probiotic bacterial strains. Aquaculture, 256 (1-4): 50–73.

Soto-Rodríguez S A, Simoes N, Roque A, Gómez Gil B. 2006. Pathogenicity and colonization of Litopenaeus vannamei larvae by luminescent vibrios. Aquaculture, 258 (1-4): 109–115.

Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S. 2011. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol. Biol. Evol., 28 (10): 2 731–2 739.

Vandenberghe J, Verdonck L, Robles-Arozarena R, Rivera G, Bolland A, Balladares M, Gomez-Gil B, Calderon J, Sorgeloos P, Swings J. 1999. Vibrios associated with Litopenaeus vannamei larvae, postlarvae, broodstock, and hatchery probionts. Appl. Environ. Microbiol., 65 (6): 2 592–2 597.

Wen C Q, Lai X T, Xue M, Huang Y L, Li H X, Zhou S N. 2009. Molecular typing and identification of Bdellovibrio-and-like organisms isolated from seawater shrimp ponds and adjacent coastal waters. J. Appl. Microbiol., 106 (4): 1 154–1 162.

Xia Z, Zhu M, Zhang Y. 2013. Effects of the probiotic Arthrobacter sp. CW9 on the survival and immune status of white shrimp (Penaeus vannamei). Lett. Appl. Microbiol., 58 (1): 60–64.

Xu H S, Yang X S, Li Y. 1999. Diagnosis and Control of Bacterial Diseases in Penaeid Shrimp Hatcheries. Ocean Press, Beijing, China. p.136–152. (in Chinese)

浏览量

Downloads

CSCD

文章被引用时，请邮件提醒。

Submit

工具集

关联资源

Description of two new species of Pseudoaliinostoc (Nostocales, Cyanobacteria) from China based on the polyphasic approach

Characterization of microbial communities in sediments of the South Yellow Sea

Bacterial and archaeal communities in deep sea waters near the Ninetyeast Ridge in Indian Ocean

High-throughput sequencing of 16S rRNA amplicons characterizes gut microbiota shift of juvenile sea cucumber Apostichopus japonicus feeding with three antibiotics

Effects of dietary supplementation of Ulva pertusa and nonstarch polysaccharide enzymes on gut microbiota of Siganus canaliculatus