Activation of macrophages by an exopolysaccharide isolated from Antarctic Psychrobacter sp. B-3

Leiye Yu; Guojie Sun; Jingfang Wei; Yingze Wang; Chao Du; Jiang Li

doi:10.1007/s00343-016-4393-x

Your Location：

Home >

Browse articles >

Activation of macrophages by an exopolysaccharide isolated from Antarctic Psychrobacter sp. B-3

OriginalPaper | Updated：2023-05-16

- Activation of macrophages by an exopolysaccharide isolated from Antarctic Psychrobacter sp. B-3
- Activation of macrophages by an exopolysaccharide isolated from Antarctic Psychrobacter sp. B-3
- 海洋湖沼学报（英文） 2016年34卷第5期页码：1064-1071
- Affiliations：
  
  1. College of Bioscience and Bioengineering Hebei University of Science and Technology
  2. Key Laboratory of Marine Bioactive Substances First Institute of Oceanography State Oceanic Administration (SOA)
- Author bio：
- Funds：
  
  Supported by the Chinese Polar Environment Comprehensive Investigation & Assessment Programs (No. CHINARE2013-01-05), the National Science & Technology Major Project (No. 2011ZX8001-003), the Hebei Province Science & Technology Support Program (No. 12220407D), and the National Natural Science Foundation of China (No. 31100715)
- DOI：10.1007/s00343-016-4393-x
  中图分类号：
- 网络首发：2016-01-09，
  
  纸质出版：2016
- Accepted：
Scan QR Code
Activation of macrophages by an exopolysaccharide isolated from Antarctic Psychrobacter sp. B-3[J]. 海洋湖沼学报（英文）, 2016,34(5):1064-1071.

Yu, L., Sun, G., Wei, J. et al. Activation of macrophages by an exopolysaccharide isolated from Antarctic Psychrobacter sp. B-3., Chin. J. Ocean. Limnol. 34, 1064–1071 (2016). https://doi.org/10.1007/s00343-016-4393-x
Activation of macrophages by an exopolysaccharide isolated from Antarctic Psychrobacter sp. B-3[J]. 海洋湖沼学报（英文）, 2016,34(5):1064-1071. DOI： 10.1007/s00343-016-4393-x.

Yu, L., Sun, G., Wei, J. et al. Activation of macrophages by an exopolysaccharide isolated from Antarctic Psychrobacter sp. B-3., Chin. J. Ocean. Limnol. 34, 1064–1071 (2016). https://doi.org/10.1007/s00343-016-4393-x DOI：

摘要

Abstract

An exopolysaccharide (EPS) was isolated and purified from an Antarctic psychrophilic bacterium B-3

identified as Psychrobacter sp.

and the activation of RAW264.7 cells by B-3 EPS was investigated. The results show that B-3 EPS

over a certain concentration range

promoted cell viability

nitric oxide production

tumor necrosis factor (TNF)α secretion

and phagocytic ability. Furthermore

TAK-242

an inhibitor of the toll-like receptor 4 (TLR4) significantly reduced nitric oxide production by these cells after stimulation with B-3 EPS. Moreover

B-3 EPS induced p65 phosphorylation and IκBα degradation in these cells. In conclusion

B-3 EPS might have activated RAW264.7 cells by combining with TLR4 on cell surface and triggering activation of NF-κB signaling pathways

implying that this EPS could activate macrophages and regulate initial immune response.

关键词

Keywords

references

Akashi S, Shimazu R, Ogata H, Nagai Y, Takeda K, Kimoto M, Miyake K. 2000. Cutting edge: cell surface expression and lipopolysaccharide signaling via the Toll-like receptor 4-MD-2 complex on mouse peritoneal macrophages. J. Immunol., 164(7): 3471–3475.

Akira S, Takeda K. 2004. Toll-like receptor signalling. Nat. Rev. Immunol., 4: 499–511.

Ando I, Tsukumo Y, Wakabayashi T, Akashi S, Miyake K, Kataoka T, Nagai K. 2002. Safflower polysaccharides activate the transcription factor NF-κB via Toll-like receptor 4 and induce cytokine production by macrophages. Int. Immunopharmacol., 2(8): 1155–1162.

Bai Y G, Zhang P Y, Chen G C, Cao J F, Huang T T, Chen K S. 2012. Macrophage Immunomodulatory activity of extracellular polysaccharide (PEP) of Antarctic bacterium Pseudoaltermonas sp. S-5. International Immunopharmacology, 12(4): 611–617.

Fang J N. 1984. The separation, purification and determination the purity and molecular weight of polysaccharide. Pharmaceutical Bulletin, 19(10): 46–49.

Hagström Å, Pommier T, Rohwer F, Simu K, Stolte W, Svensson D, Zweifel U L. 2002. Use of 16S ribosomal DNA for delineation of marine bacterioplankton species. Appl. Environ. Microbiol., 68(7): 3628–3633.

Hayden M S, Ghosh S. 2004. Signaling to NF-κB. Genes. Dev., 18(18): 2195–2224.

Hayden M S, Ghosh S. 2008. Shared principles in NF-κB Signaling. Cell, 132(3): 344–362.

Kawai T, Akira S. 2011. Toll-like receptors and their crosstalk with other innate receptors in infection and immunity. Immunity, 34(5): 637–650.

Kawamoto T, Ii M, Kitazaki T, Iizawa Y, Kimura H. 2008 TAK-242 selectively suppresses Toll-like receptor 4-signaling mediated by the intracellular domain. Eur. J. Pharmacol., 584(1): 40–48.

Kitazawa H, Ishii Y, Uemura J, Kawai Y, Saito T, Kaneko T, Noda K, Itoh T. 2000. Augmentation of macrophage functions by an extracellular phosphopolysaccharide from Lactobacillus Delbrueckii ssp. bulgaricus. Food. Microbiol., 17(1): 109–118.

Leung M Y K, Liu C, Koon J C M, Fung K P. 2006. Polysaccharide biological response modifiers. Immunol. Lett., 105(2): 101–114.

Li B, Cramer D, Wagner S, Hansen R, King C, Kakar S, Ding C L, Yan J. 2007. Yeast glucan particles activate murine resident macrophages to secrete proinflammatory cytokines via MyD88- and Syk kinase dependent pathways. Clin. Immuno l., 124(2): 170–181.

Li T L, Wu C X, Zhang Y X. 1982. Studies of gas chromatographic analysis of saccharides and alditols II: some improvements in the analysis of acetylated aldononitriles by gas chromatography. Chinese Journal of Analytical Chemistry, 10(5): 272–276.

Murray P J, Wynn T A. 2011. Protective and pathogenic functions of macrophage subsets. Nat. Rev. Immunol., 11(11): 723–737.

Naumann M, Scheidereit C. 1994. Activation of NF-κB in vivo is regulated by multiple phosphorylations. EMBO J., 13(19): 4597–4607.

Neumann M, Grieshammer T, Chuvpilo S, Kneitz B, Lohoff M, Schimpl A, Franza BR Jr, Serfling E. 1995. RelA/p65 is a molecular target for the immunosuppressive action of protein kinase A. EMBO J., 14(9): 1991–2004.

Nichols C A M, Guezennec J, Bowman J P. 2005. Bacterial exopolysaccharides from extreme marine environments with special consideration of the Southern Ocean, sea ice, and deep-sea hydrothermal vents: a review. Mar. Biotechnol., 7(4): 253–271.

Nunnery J K, Mevers E, Gerwick W H. 2010. Biologically active secondary metabolites from marine cyanobacteria. Curr. Opin. Biotechnol., 21(6): 787–793.

Ramberg J E, Nelson E D, Sinnott R A. 2010. Immunomodulatory dietary polysaccharides: a systematic review of the literature. Nutrition Journal, 9: 54.

Schepetkin I A, Quinn M T. 2006. Botanical polysaccharides: macrophage immunomodulation and therapeutic potential. Int. J. Immunopharmacol., 6(3): 317–333.

Senni K, Pereira J, Gueniche F, Delbarre-Ladrat C, Sinquin C, Ratiskol J, Godeau G, Fischer A M, Helley D, Colliec-Jouault S. 2011. Marine polysaccharides: a source of bioactive molecules for cell therapy and tissue engineering. Mar. Drugs., 9(9): 1664–1681.

Sun S C. 2012. The noncanonical NF-κB pathway. Immunol. Rev., 246(1): 125–140.

Takeuchi O, Akira S. 2010. Pattern recognition receptors and inflammation. Cell, 140(6): 805–820.

Tan L T. 2007. Bioactive natural products from marine cyanobacteria for drug discovery. Phytochemistry, 68(7): 954–979.

Wasser S P. 2002. Medicinal mushrooms as a source of antitumor and immunomodulating polysaccharides. Appl. Microbiol. Biotechnol., 60(3): 258–274.

Wynn T A, Chawla A, Pollard J W. 2013. Macrophage biology in development, homeostasis and disease. Nature, 496(7446): 445–455.

Yoshimura T, Kurita C, Hayata M, Nagai H. 1993. Diagnosis of drug allergy by the lymphocyte stimulation test with the MTT [3-(4, 5-dimethyl thiazol-2-yl)-2, 5-diphenyl tetrazolium bromide] assay. Biol. Pharm. Bull., 16(7): 686–689.

浏览量

Downloads

CSCD

文章被引用时，请邮件提醒。

Submit

工具集

关联资源

暂无数据