Homologous cloning, characterization and expression of a new halophyte phytochelatin synthase gene in Suaeda salsa

Ming Cong; Jianmin Zhao; Jiasen Lü; Zhiming Ren; Huifeng Wu

doi:10.1007/s00343-016-4382-0

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Homologous cloning, characterization and expression of a new halophyte phytochelatin synthase gene in Suaeda salsa

OriginalPaper | Updated：2023-05-16

- Homologous cloning, characterization and expression of a new halophyte phytochelatin synthase gene in Suaeda salsa
- Homologous cloning, characterization and expression of a new halophyte phytochelatin synthase gene in Suaeda salsa
- 海洋湖沼学报（英文） 2016年34卷第5期页码：1034-1043
- Affiliations：
  
  1. Key Laboratory of Coastal Zone Environmental Processes Yantai Institute of Coastal Zone Research (YIC) Chinese Academy of Sciences (CAS) Shandong Provincial Key Laboratory of Coastal Zone Environmental Processes YICCAS
  2. Biology School of Yantai University
- Author bio：
- Funds：
  
  Supported by the 100 Talents Program of the Chinese Academy of Sciences and the Key Technology R&D Program of Shandong Province (No. 2012GGA06032)
- DOI：10.1007/s00343-016-4382-0
  中图分类号：
- 网络首发：2016-01-28，
  
  纸质出版：2016
- Accepted：
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Homologous cloning, characterization and expression of a new halophyte phytochelatin synthase gene in Suaeda salsa[J]. 海洋湖沼学报（英文）, 2016,34(5):1034-1043.

Cong, M., Zhao, J., Lü, J. et al. Homologous cloning, characterization and expression of a new halophyte phytochelatin synthase gene in Suaeda salsa, ., Chin. J. Ocean. Limnol. 34, 1034–1043 (2016). https://doi.org/10.1007/s00343-016-4382-0
Homologous cloning, characterization and expression of a new halophyte phytochelatin synthase gene in Suaeda salsa[J]. 海洋湖沼学报（英文）, 2016,34(5):1034-1043. DOI： 10.1007/s00343-016-4382-0.

Cong, M., Zhao, J., Lü, J. et al. Homologous cloning, characterization and expression of a new halophyte phytochelatin synthase gene in Suaeda salsa, ., Chin. J. Ocean. Limnol. 34, 1034–1043 (2016). https://doi.org/10.1007/s00343-016-4382-0 DOI：

摘要

Abstract

The halophyte Suaeda salsa can grow in heavy metal-polluted areas along intertidal zones having high salinity. Since phytochelatins can eff ectively chelate heavy metals

it was hypothesized that S. salsa possessed a phytochelatin synthase (PCS) gene. In the present study

the cDNA of PCS was obtained from S. salsa (designated as SsPCS) using homologous cloning and the rapid amplification of cDNA ends (RACE). A sequence analysis revealed that SsPCS consisted of 1 916 bp nucleotides

encoding a polypeptide of 492 amino acids with one phytochelatin domain and one phytochelatin C domain. A similarity analysis suggested that SsPCS shared up to a 58.6% identity with other PCS proteins and clustered with PCS proteins from eudicots. There was a new kind of metal ion sensor motif in its C-terminal domain. The SsPCS transcript was more highly expressed in elongated and fibered roots and stems (P<0.05) than in leaves. Lead and mercury exposure significantly enhanced the mRNA expression of SsPCS (P<0.05). To the best of our knowledge

SsPCS is the second PCS gene cloned from a halophyte

and it might contain a diff erent metal sensing capability than the first PCS from Thellungiella halophila. This study provided a new view of halophyte PCS genes in heavy metal tolerance.

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references

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