Low temperature stress on the hematological parameters and HSP gene expression in the turbot Scophthalmus maximus

Liqin Ji; Keyong Jiang; Mei Liu; Baojie Wang; Longjiang Han; Mingming Zhang; Lei Wang

doi:10.1007/s00343-016-4367-z

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Low temperature stress on the hematological parameters and HSP gene expression in the turbot Scophthalmus maximus

OriginalPaper | Updated：2023-05-16

- Low temperature stress on the hematological parameters and HSP gene expression in the turbot Scophthalmus maximus
- Low temperature stress on the hematological parameters and HSP gene expression in the turbot Scophthalmus maximus
- 海洋湖沼学报（英文） 2016年34卷第3期页码：430-440
- Affiliations：
  
  1. Key Laboratory of Experimental Marine Biology Chinese Academy of Sciences
  2. University of Chinese Academy of Sciences
  3. Fisheries College Ocean University of China
- Author bio：
- Funds：
  
  Supported by the Special Foundation for Youth Science and Technology of Qingdao (No. 14-2-4-59-jch), the Science and Technology Development Planning of Shandong Province (No. 2011GGC21007), and the National Natural Science Foundation of China (No. 41206144).
- DOI：10.1007/s00343-016-4367-z
  中图分类号：
- 网络首发：2015-10-21，
  
  纸质出版：2016
- Accepted：
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Low temperature stress on the hematological parameters and HSP gene expression in the turbot Scophthalmus maximus[J]. 海洋湖沼学报（英文）, 2016,34(3):430-440.

Ji, L., Jiang, K., Liu, M. et al. Low temperature stress on the hematological parameters and HSP gene expression in the turbot Scophthalmus maximus, ., Chin. J. Ocean. Limnol. 34, 430–440 (2016). https://doi.org/10.1007/s00343-016-4367-z
Low temperature stress on the hematological parameters and HSP gene expression in the turbot Scophthalmus maximus[J]. 海洋湖沼学报（英文）, 2016,34(3):430-440. DOI： 10.1007/s00343-016-4367-z.

Ji, L., Jiang, K., Liu, M. et al. Low temperature stress on the hematological parameters and HSP gene expression in the turbot Scophthalmus maximus, ., Chin. J. Ocean. Limnol. 34, 430–440 (2016). https://doi.org/10.1007/s00343-016-4367-z DOI：

摘要

Abstract

To study the effect of low temperature stress on hematological parameters and HSP gene expression in the turbot (Scophthalmus maximus)

water temperature was lowered rapidly from 18 to 1°C. During the cooling process

three individuals were removed from culture tanks at 18

and 1°C. Blood samples and tissues were taken from each individual

hematological indices and HSP gene expression in tissues were measured. The red blood cell count

white blood cell count

and hemoglobin concentration decreased significantly (P < 0.05) as temperature decreased. Enzyme activities of plasma alanine transaminase and creatine kinase increased as temperature decreased

whereas aspartic transaminase and γ-glutamyl transpeptidase activities displayed no obvious changes above 1°C and lactate dehydrogenase activity increased first and then decreased. Blood urea nitrogen and uric acid levels were highest at 8°C

and creatinine concentration was highest at 3°C. The concentrations of plasma cortisol

cholesterol

and triglyceride all increased significantly (P < 0.05) as temperature decreased. The serum glucose concentration increased first and then decreased to the initial level. The HSP70 mRNA expression showed various patterns in different tissues

whereas HSP90 mRNA expression showed the same tendency in all tissues. Overall

these results indicate that temperature decreases in the range of 8 to 5°C may induce a stress response in S. maximus and that temperature should be kept above 8°C in the aquaculture setting to avoid damage to the fish.

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references

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