Optimization of four types of antimicrobial agents to increase the inhibitory ability of marine Arthrobacter oxydans KQ11 dextranase mouthwash

Wei Ren; Shujun Wang; Mingsheng Lü; Xiaobei Wang; Yaowei Fang; Yuliang Jiao; Jianen Hu

doi:10.1007/s00343-015-4376-3

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Optimization of four types of antimicrobial agents to increase the inhibitory ability of marine Arthrobacter oxydans KQ11 dextranase mouthwash

OriginalPaper | Updated：2023-05-16

- Optimization of four types of antimicrobial agents to increase the inhibitory ability of marine Arthrobacter oxydans KQ11 dextranase mouthwash
- Optimization of four types of antimicrobial agents to increase the inhibitory ability of marine Arthrobacter oxydans KQ11 dextranase mouthwash
- 海洋湖沼学报（英文） 2016年34卷第2期页码：354-366
- Affiliations：
  
  1. College of Food Science and Engineering Dalian Ocean University
  2. Jiangsu Marine Resources Development Research Institute
  3. Jiangsu Key Laboratory of Marine Pharmaceutical Compound Screening Huaihai Institute of Technology
  4. Innovation Center of Jiangsu Marine Bio - Industry Technology Huaihai Institute of Technology,Co
- Author bio：
- Funds：
  
  Supported by the National Natural Science Foundation of China (Nos. 31471719, 31271929), the Jiangsu Province and Technology Support Program (No. BE2013662), the Lianyungang Fifth “521 High-Level Talent Training Project”, the Priority Academic Program Development of Jiangsu Higher Education Institutions
- DOI：10.1007/s00343-015-4376-3
  中图分类号：
- 网络首发：2015-11-24，
  
  纸质出版：2016
- Accepted：
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Optimization of four types of antimicrobial agents to increase the inhibitory ability of marine Arthrobacter oxydans KQ11 dextranase mouthwash[J]. 海洋湖沼学报（英文）, 2016,34(2):354-366.

Ren, W., Wang, S., Lü, M. et al. Optimization of four types of antimicrobial agents to increase the inhibitory ability of marine Arthrobacter oxydans KQ11 dextranase mouthwash., Chin. J. Ocean. Limnol. 34, 354–366 (2016). https://doi.org/10.1007/s00343-015-4376-3
Optimization of four types of antimicrobial agents to increase the inhibitory ability of marine Arthrobacter oxydans KQ11 dextranase mouthwash[J]. 海洋湖沼学报（英文）, 2016,34(2):354-366. DOI： 10.1007/s00343-015-4376-3.

Ren, W., Wang, S., Lü, M. et al. Optimization of four types of antimicrobial agents to increase the inhibitory ability of marine Arthrobacter oxydans KQ11 dextranase mouthwash., Chin. J. Ocean. Limnol. 34, 354–366 (2016). https://doi.org/10.1007/s00343-015-4376-3 DOI：

摘要

Abstract

We adopted the response surface methodology using single factor and orthogonal experiments to optimize four types of antimicrobial agents that could inhibit biofilm formation by Streptococcus mutans

which is commonly found in the human oral cavity and causes tooth decay. The objective was to improve the function of marine Arthrobacter oxydans KQ11 dextranase mouthwash (designed and developed by our laboratory). The experiment was conducted in a three-level

four-variable central composite design to determine the best combination of ZnSO4

lysozyme

citric acid and chitosan. The optimized antibacterial agents were 2.16 g/L ZnSO4

14 g/L lysozyme

4.5 g/L citric acid and 5 g/L chitosan. The biofilm formation inhibition reached 84.49%. In addition

microscopic observation of the biofilm was performed using scanning electron microscopy and confocal laser scanning microscopy. The optimized formula was tested in marine dextranase Arthrobacter oxydans KQ11 mouthwash and enhanced the inhibition of S. mutans. This work may be promoted for the design and development of future marine dextranase oral care products.

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references

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