Investigation into the hemolytic activity of tentacle venom from jellyfish Cyanea nozakii Kishinouye

Cuiping Li; Huahua Yu; Rongfeng Li; Ronge Xing; Song Liu; Pengcheng Li

doi:10.1007/s00343-015-4322-4

Your Location：

Home >

Browse articles >

Investigation into the hemolytic activity of tentacle venom from jellyfish Cyanea nozakii Kishinouye

OriginalPaper | Updated：2023-05-16

- Investigation into the hemolytic activity of tentacle venom from jellyfish Cyanea nozakii Kishinouye
- Investigation into the hemolytic activity of tentacle venom from jellyfish Cyanea nozakii Kishinouye
- 海洋湖沼学报（英文） 2016年34卷第2期页码：382-385
- Affiliations：
  
  1. Qingdao University of Technology
  2. Key Laboratory of Experimental Marine Biology Institute of Oceanology Chinese Academy of Sciences
- Author bio：
- Funds：
  
  Supported by the National Natural Science Foundation of China (Nos. 41376004, 41406152), the Key R&D program of Shandong Province (Nos. 2015GSF115022, 2015GSF115034) and the China Postdoctoral Science Foundation (No. 2013M531654)
- DOI：10.1007/s00343-015-4322-4
  中图分类号：
- 网络首发：2016-01-09，
  
  纸质出版：2016
- Accepted：
Scan QR Code
Investigation into the hemolytic activity of tentacle venom from jellyfish Cyanea nozakii Kishinouye[J]. 海洋湖沼学报（英文）, 2016,34(2):382-385.

Li, C., Yu, H., Li, R. et al. Investigation into the hemolytic activity of tentacle venom from jellyfish Cyanea nozakii Kishinouye., Chin. J. Ocean. Limnol. 34, 382–385 (2016). https://doi.org/10.1007/s00343-015-4322-4
Investigation into the hemolytic activity of tentacle venom from jellyfish Cyanea nozakii Kishinouye[J]. 海洋湖沼学报（英文）, 2016,34(2):382-385. DOI： 10.1007/s00343-015-4322-4.

Li, C., Yu, H., Li, R. et al. Investigation into the hemolytic activity of tentacle venom from jellyfish Cyanea nozakii Kishinouye., Chin. J. Ocean. Limnol. 34, 382–385 (2016). https://doi.org/10.1007/s00343-015-4322-4 DOI：

摘要

Abstract

Cyanea nozakii Kishinouy e (C. nozakii)

a giant cnidarian of the class Scyphomedusae

order Semaeostomeae and family Cyaneidae

is widely distributed in the East China Sea

the Yellow Sea and the Bohai Sea

and is abundant from late summer to early autumn. Venom produced by C. nozakii during mass agglomerations can contaminate seawater resulting in death of the halobios and seriously damage commercial fisheries. Swimmers and fishermen commonly suff er painful stings from this jellyfish

resulting in local edema

tingling

breathing difficulties

depressed blood pressure and even death. Such effects arise from the complex mixture of biologically active molecules that make up jellyfish venom. In the present study

the hemolytic activity of venom from tentacles of C. nozakii and factors aff ecting its activity were assayed. The HU50 ( defined as the amount of protein required to lyse 50 % of erythrocytes) of the venom against dove and chicken erythrocytes was 34 and 59 μg/mL

respectively. Carboxylmethyl chitosan and glycerol could increase hemolytic activity at concentrations greater than 0.06% and 0.2 mol/L

respectively.

关键词

Keywords

references

Bradford M M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem., 72 (1-2): 248–254.

Chung J J, Ratnapala L A, Cooke I M, Yanagihara A A. 2001. Partial purification and characterization of a hemolysin (CAH1) from Hawaiian box jellyfish (Carybdea alata) venom. Toxicon, 39 (7): 981–990.

Dong J, Liu C Y, Li W Q, Yu H B, Wang B, Wang Y Q. 2005. The morphology and structure of jellyfish (Cyanea nozakii Kishinouye). Fish. Sci., 24 (2): 22–23. (in Chinese with English abstract)

Feng J H, Yu H H, Li C P, Xing R E, Liu S, Wang L, Cai S B, Li P C. 2010a. Isolation and characterization of lethal proteins in nematocyst venom of the jellyfish Cyanea nozakii Kishinouye. Toxicon, 55 (1): 118–125.

Feng J H, Yu H H, Xing R E, Liu S, Wang L, Cai S B, Li P C. 2010b. Partial characterization of the hemolytic activity of the nematocyst venom from the jellyfish Cyanea nozakii Kishinouye. Toxico l. in Vitro, 24 (6): 1750–1756.

Gusmani L, Avian M, Galil B, Patriarca P, Rottini G. 1997. Biologically active polypeptides in the venom of the jellyfish Rhopilema nomadica. Toxicon, 35 (5): 637–648.

Li C P, Yu H H, Liu S, Xing R E, Guo Z Y, Li P C. 2005. Factors aff ecting the protease activity of venom from jellyfish Rhopilema esculentum Kishinouye. Bioorg. Med. Chem. Lett., 15 (24): 5370–5374.

Li R F, Yu H H, Xing R E, Liu S, Qing Y K, Li K C, Li B, Meng X T, Cui J H, Li P C. 2013. Isolation and in vitro partial characterization of hemolytic proteins from the nematocyst venom of the jellyfish Stomolophus meleagris. Toxicol. in Vitro., 27(6): 1 620–1 625.

Radwan F F Y, Burnett J W, Bloom D A, Coliano T, Eldefrawi M E, Erderly H, Aurelian L, Torres M, Heimer-de la Cotera E P. 2001. A comparison of the toxinological characteristics of two Cassiopea and Aurelia species. Toxicon, 39 (2-3): 245–257.

Radwan F F Y, Gershwin L A, Burnett J W. 2000. Toxinological studies on the nematocyst venom of Chrysaora achlyos. Toxicon, 38 (11): 1581–1591.

Radwan F F Y, Román L G, Baksi K, Burnett J W. 2005. Toxicity and mAChRs binding activity of Cassiopea xamachana venom from Puerto Rican coasts. Toxicon, 45 (1): 107–112.

Ramasamy S, Isbister G K, Seymour J E, Hodgson W C. 2003. The in vitro Effects of two chirodropid (Chironex fleckeri and Chiropsalmus sp.) venoms: efficacy of box jellyfish antivenom. Toxicon, 41 (6): 703–711.

Rottini G, Gusmani L, Parovel E, Avian M, Patriarca P. 1995. Purification and properties of a cytolytic toxin in venom of the jellyfish Carybdea marsupi a lis. Toxicon, 33 (3): 315–326.

Torres M, Aguilar M B, Falcón A, Sánchez L, Radwan F F Y, Burnett J W, Heimer-de la Cotera E P, Arellano R O. 2001. Electrophysiological and hemolytic activity elicited by the venom of the jellyfish Cassiopea xamachana. Toxicon, 39 (9): 1297–1307.

Uechi G, Toma H, Arakawa T, Sato Y. 2005. Biochemical and physiological analyses of a hemolytic toxin isolated from a sea anemone Actineria villosa. Toxicon, 45 (6): 761–766.

Winter K L, Isbister G K, Seymour J E, Hodgson WC. 2007. An in vivo examination of the stability of venom from the Australian box jellyfish Chironex fleckeri. Toxicon, 49 (6): 804–809.

Xiao L, He Q, Guo Y F, Zhang J, Nie F, Li Y, Ye X F, Zhang L M. 2009. Cyanea capillata tentacle-only extract as a potential alternative of nematocyst venom: its cardiovascular toxicity and tolerance to isolation and purification procedures. Toxicon, 53 (1): 146–152.

Yu H H, Li C P, Li R G, Xing R E, Liu S, Li P C. 2007. Factors influencing hemolytic activity of venom from the jellyfish Rhopilema Esculentum Kishinouye. Food Chem. Toxico l., 45 (7): 1173–1178.

Yu H H, Li R F, Dong X L, Xing R E, Liu S, Li P C. 2014. Efficacy of venom from tentacle of jellyfish Stomolophus meleagris (Nemopilema nomurai) against the cotton bollworm Helicoverpa armigera. Biomed Res. Int., 2014: 315853, http://dx.doi.org/10.1155/2014/315853.

Yu H H, Liu X G, Dong X L, Li C P, Xing R E, Liu S, Li P C. 2005. Insecticidal activity of proteinous venom from tentacle of jellyfish Rhopilema esculentum Kishinouye. Bioorg. Med. Chem. Lett., 15 (22): 4949–4952.

Yu H H, Liu X G, Xing R E, Guo Zh Y, Wang P B, Li C P, Li P C. 2006. In vitro determination of antioxidant activity of proteins from jellyfish Rhopilema esculentum. Food Chem., 95 (1): 123–130.

Zhong X M, Tang J H, Liu P T. 2004. A study on the relationship between Cyanea nozakii Kishinouye breaking out and ocean ecosystem. Mod. Fish. Inf., 19 (3): 15–17. (in Chinese with English abstract)

浏览量

Downloads

CSCD

文章被引用时，请邮件提醒。

Submit

工具集

关联资源

Comparative study of the hemolytic and cytotoxic activities of nematocyst venoms from the jellyfish Cyanea nozakii Kishinouye and Nemopilema nomurai Kishinouye

Comparative analysis of methods for concentrating venom from jellyfish Rhopilema esculentum Kishinouye

Molecular monitoring plankton community during jellyfish blooms in the water near the Liaoning Hongyan River Nuclear Power Plant

Stress regulation of photosynthetic system of Phaeocystis globosa and their hemolytic activity

Inductive effect of bioactive substances on strobilation of jellyfish Aurelia coerulea