Iodothyronine deiodinase gene analysis of the Pacific oyster Crassostrea gigas reveals possible conservation of thyroid hormone feedback regulation mechanism in mollusks

Wen Huang; Fei Xu; Tao Qu; Li Li; Huayong Que; Guofan Zhang

doi:10.1007/s00343-015-4300-x

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Iodothyronine deiodinase gene analysis of the Pacific oyster Crassostrea gigas reveals possible conservation of thyroid hormone feedback regulation mechanism in mollusks

OriginalPaper | Updated：2023-05-16

- Iodothyronine deiodinase gene analysis of the Pacific oyster Crassostrea gigas reveals possible conservation of thyroid hormone feedback regulation mechanism in mollusks
- Iodothyronine deiodinase gene analysis of the Pacific oyster Crassostrea gigas reveals possible conservation of thyroid hormone feedback regulation mechanism in mollusks
- 海洋湖沼学报（英文） 2015年33卷第4期页码：997-1006
- Affiliations：
  
  1. National & Local Joint Engineering Laboratory of Ecological Mariculture Institute of Oceanology Chinese Academy of Sciences
  2. University of Chinese Academy of Sciences
- Author bio：
- Funds：
  
  Supported by the National Natural Science Foundation of China (Nos. 31372515, 31402285), the National Basic Research Program of China (973 Program) (No. 2010CB126401), and the National High Technology Research and Development Program (863 Program) (No. 2012AA10A405)
- DOI：10.1007/s00343-015-4300-x
  中图分类号：
- 网络首发：2015-04-10，
  
  纸质出版：2015
- Accepted：
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Iodothyronine deiodinase gene analysis of the Pacific oyster Crassostrea gigas reveals possible conservation of thyroid hormone feedback regulation mechanism in mollusks[J]. 海洋湖沼学报（英文）, 2015,33(4):997-1006.

Huang, W., Xu, F., Qu, T. et al. Iodothyronine deiodinase gene analysis of the Pacific oyster Crassostrea gigas reveals possible conservation of thyroid hormone feedback regulation mechanism in mollusks., Chin. J. Ocean. Limnol. 33, 997–1006 (2015). https://doi.org/10.1007/s00343-015-4300-x
Iodothyronine deiodinase gene analysis of the Pacific oyster Crassostrea gigas reveals possible conservation of thyroid hormone feedback regulation mechanism in mollusks[J]. 海洋湖沼学报（英文）, 2015,33(4):997-1006. DOI： 10.1007/s00343-015-4300-x.

Huang, W., Xu, F., Qu, T. et al. Iodothyronine deiodinase gene analysis of the Pacific oyster Crassostrea gigas reveals possible conservation of thyroid hormone feedback regulation mechanism in mollusks., Chin. J. Ocean. Limnol. 33, 997–1006 (2015). https://doi.org/10.1007/s00343-015-4300-x DOI：

摘要

Abstract

Iodothyronine deiodinase catalyzes the initiation and termination of thyroid hormones (THs) effects

and plays a central role in the regulation of thyroid hormone level in vertebrates. In non-chordate invertebrates

only one deiodinase has been identified in the scallop Chlamys farreri. Here

two deiodinases were cloned in the Pacific oyster Crassostrea gigas (CgDx and CgDy). The characteristic in-frame TGA codons and selenocysteine insertion sequence elements in the oyster deiodinase cDNAs supported the activity of them. Furthermore

seven orthologs of deiodinases were found by a tblastn search in the mollusk Lottia gigantea and the annelid Capitella teleta. A phylogenetic analysis revealed that the deiodinase gene originated from an common ancestor and a clade-specific gene duplication occurred independently during the differentiation of the mollusk

annelid

and vertebrate lineages. The distinct spatiotemporal expression patterns implied functional divergence of the two deiodinases. The expression of CgDx and CgDy was influenced by L-thyroxine T4

and putative thyroid hormone responsive elements were found in their promoters

which suggested that the oyster deiodinases were feedback regulated by TH. Epinephrine stimulated the expression level of CgDx and CgDy

suggesting an interaction effect between different hormones. This study provides the first evidence for the existence of a conserved TH feedback regulation mechanism in mollusks

providing insights into TH evolution.

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references

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