A method of batch-purifying microalgae with multiple antibiotics at extremely high concentrations

Jichang Han; Song Wang; Lin Zhang; Guanpin Yang; Lu Zhao; Kehou Pan

doi:10.1007/s00343-015-4288-2

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A method of batch-purifying microalgae with multiple antibiotics at extremely high concentrations

OriginalPaper | Updated：2023-05-16

- A method of batch-purifying microalgae with multiple antibiotics at extremely high concentrations
- A method of batch-purifying microalgae with multiple antibiotics at extremely high concentrations
- 海洋湖沼学报（英文） 2016年34卷第1期页码：79-85
- Affiliations：
  
  1. Laboratory of Applied Microalgae Biology Key Laboratory of Aquaculture of Ministry of Education Ocean University of China
  2. College of Marine Life Sciences Ocean University of China
- Author bio：
- Funds：
  
  Supported by the National Basic Research Program of China (973 Program) (No. 2011CB200901), the National Technical Supporting Project Foundation (No. 2011BAD14B01), and the National High Technology Research and Development Program of China (863 Program) (No. 2013AA065801)
- DOI：10.1007/s00343-015-4288-2
  中图分类号：
- 网络首发：2015-06-18，
  
  纸质出版：2016
- Accepted：
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A method of batch-purifying microalgae with multiple antibiotics at extremely high concentrations[J]. 海洋湖沼学报（英文）, 2016,34(1):79-85.

Han, J., Wang, S., Zhang, L. et al. A method of batch-purifying microalgae with multiple antibiotics at extremely high concentrations., Chin. J. Ocean. Limnol. 34, 79–85 (2016). https://doi.org/10.1007/s00343-015-4288-2
A method of batch-purifying microalgae with multiple antibiotics at extremely high concentrations[J]. 海洋湖沼学报（英文）, 2016,34(1):79-85. DOI： 10.1007/s00343-015-4288-2.

Han, J., Wang, S., Zhang, L. et al. A method of batch-purifying microalgae with multiple antibiotics at extremely high concentrations., Chin. J. Ocean. Limnol. 34, 79–85 (2016). https://doi.org/10.1007/s00343-015-4288-2 DOI：

摘要

Abstract

Axenic microalgal strains are highly valued in diverse microalgal studies and applications. Antibiotics

alone or in combination

are often used to avoid bacterial contamination during microalgal isolation and culture. In our preliminary trials

we found that many microalgae ceased growing in antibiotics at extremely high concentrations but could resume growth quickly when returned to an antibiotics-free liquid medium and formed colonies when spread on a solid medium. We developed a simple and highly efficient method of obtaining axenic microalgal cultures based on this observation. First

microalgal strains of different species or strains were treated with a mixture of ampicillin

gentamycin sulfate

kanamycin

neomycin and streptomycin (each at a concentration of 600 mg/L) for 3 days; they were then transferred to antibiotics-free medium for 5 days; and finally they were spread on solid f/2 media to allow algal colonies to form. With this method

five strains of Nannochloropsis sp. (Eustigmatophyceae)

two strains of Cylindrotheca sp. (Bacillariophyceae)

two strains of Tetraselmis sp. (Chlorodendrophyceae) and one strain of Amphikrikos sp. (Trebouxiophyceae) were purified successfully. The method shows promise for batch-purifying microalgal cultures.

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references

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