Microanalysis and preliminary pharmacokinetic studies of a sulfated polysaccharide from Laminaria japonica

Wenjing Zhang; Delin Sun; Xia Zhao; Weihua Jin; Jing Wang; Quanbin Zhang

doi:10.1007/s00343-015-4273-9

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Microanalysis and preliminary pharmacokinetic studies of a sulfated polysaccharide from Laminaria japonica

OriginalPaper | Updated：2023-05-16

- Microanalysis and preliminary pharmacokinetic studies of a sulfated polysaccharide from Laminaria japonica
- Microanalysis and preliminary pharmacokinetic studies of a sulfated polysaccharide from Laminaria japonica
- 海洋湖沼学报（英文） 2016年34卷第1期页码：177-185
- Affiliations：
  
  1. Institute of Oceanology Chinese Academy of Sciences
  2. University of Chinese Academy of Sciences
  3. Heze Junxinyuan Food Company
  4. Ocean University of China
- Author bio：
- Funds：
  
  Supported by the National Natural Science Foundation of China (No. 41376166), the Ocean Public Welfare Scientific Research Project (Nos. 201005024, 201405040), the Jiangsu Science and Technology Project (No. BE2012687), and the Special Fund for Cooperation between Jilin Province and Chinese Academy of Sciences (No. 2013SYHZ0023)
- DOI：10.1007/s00343-015-4273-9
  中图分类号：
- 网络首发：2015-06-17，
  
  纸质出版：2016
- Accepted：
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Microanalysis and preliminary pharmacokinetic studies of a sulfated polysaccharide from Laminaria japonica[J]. 海洋湖沼学报（英文）, 2016,34(1):177-185.

Zhang, W., Sun, D., Zhao, X. et al. Microanalysis and preliminary pharmacokinetic studies of a sulfated polysaccharide from Laminaria japonica, ., Chin. J. Ocean. Limnol. 34, 177–185 (2016). https://doi.org/10.1007/s00343-015-4273-9
Microanalysis and preliminary pharmacokinetic studies of a sulfated polysaccharide from Laminaria japonica[J]. 海洋湖沼学报（英文）, 2016,34(1):177-185. DOI： 10.1007/s00343-015-4273-9.

Zhang, W., Sun, D., Zhao, X. et al. Microanalysis and preliminary pharmacokinetic studies of a sulfated polysaccharide from Laminaria japonica, ., Chin. J. Ocean. Limnol. 34, 177–185 (2016). https://doi.org/10.1007/s00343-015-4273-9 DOI：

摘要

Abstract

A rapid

sensitive and reproducible high performance liquid chromatography (HPLC) method with post-column fluorescence derivatization has been developed to determine the amount of low-molecular-weight sulfated polysaccharide (GFS) in vivo. The metabolism of GFS has been shown to fit a two component model following its administration by intravenous injection

and its pharmacokinetic parameters were determined to be as follows: half-time of distribution phase (t 1/2α)=11.24±2.93 min

half-time of elimination phase (t 1/2β)=98.20±25.78 min

maximum concentration (C max)=110.53 μg/mL and peak time (T max)=5 min. The pharmacokinetic behavior of GFS was also investigated following intragastric administration. However

the concentration of GFS found in serum was too low for detection

and GFS could only be detected for up to 2 h after intragastric administration (200 mg/kg body weight). Thus

the bioavailability of GFS was low following intragastric administration because of the metabolism of GFS. In conclusion

HPLC with postcolumn derivatization could be used for quantitative microanalysis and pharmacokinetic studies to determine the presence of polysaccharides in the serum following intravenous injection.

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references

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