Development and evaluation of a DNA microarray assay for the simultaneous detection of nine harmful algal species in ship ballast and seaport waters

Xianfeng Chen; Qianjin Zhou; Weijun Duan; Chengxu Zhou; Lijun Duan; Huili Zhang; Aili Sun; Xiaojun Yan; Jiong Chen

doi:10.1007/s00343-015-4265-9

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Development and evaluation of a DNA microarray assay for the simultaneous detection of nine harmful algal species in ship ballast and seaport waters

OriginalPaper | Updated：2023-05-16

- Development and evaluation of a DNA microarray assay for the simultaneous detection of nine harmful algal species in ship ballast and seaport waters
- Development and evaluation of a DNA microarray assay for the simultaneous detection of nine harmful algal species in ship ballast and seaport waters
- 海洋湖沼学报（英文） 2016年34卷第1期页码：86-101
- Affiliations：
  
  1. Laboratory of Biochemistry and Molecular Biology School of Marine Sciences Ningbo University
  2. The Donghai Sea Collaborative Innovation Center for Industrial Upgrading Mariculture Ningbo University
  3. Ningbo Academy of Inspection and Quarantine
- Author bio：
- Funds：
  
  Supported by the National High Technology Research and Development Program of China (863 Program) (No. 2012AA092001), the Science and Technology Project of Zhejiang Province (No. 2013C03045-1), the Zhejiang Marine Biotechnology Innovation Team (No. 2010R50029-12), the Natural Science Foundation of Ningbo City of China (No. 2013A610168), and the KC Wong Magna Fund in Ningbo University
- DOI：10.1007/s00343-015-4265-9
  中图分类号：
- 网络首发：2015-06-04，
  
  纸质出版：2016
- Accepted：
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Development and evaluation of a DNA microarray assay for the simultaneous detection of nine harmful algal species in ship ballast and seaport waters[J]. 海洋湖沼学报（英文）, 2016,34(1):86-101.

Chen, X., Zhou, Q., Duan, W. et al. Development and evaluation of a DNA microarray assay for the simultaneous detection of nine harmful algal species in ship ballast and seaport waters., Chin. J. Ocean. Limnol. 34, 86–101 (2016). https://doi.org/10.1007/s00343-015-4265-9
Development and evaluation of a DNA microarray assay for the simultaneous detection of nine harmful algal species in ship ballast and seaport waters[J]. 海洋湖沼学报（英文）, 2016,34(1):86-101. DOI： 10.1007/s00343-015-4265-9.

Chen, X., Zhou, Q., Duan, W. et al. Development and evaluation of a DNA microarray assay for the simultaneous detection of nine harmful algal species in ship ballast and seaport waters., Chin. J. Ocean. Limnol. 34, 86–101 (2016). https://doi.org/10.1007/s00343-015-4265-9 DOI：

摘要

Abstract

Rapid

high-throughput and reliable methods are urgently required to accurately detect and monitor harmful algae

which are responsible for algal blooms

such as red and green tides. In this study

we successfully developed a multiplex PCR-based DNA microarray method capable of detecting nine harmful algal species simultaneously

namely Alexandrium tamarense

Gyrodinium instriatum

Heterosigma akashiwo

Karenia mikimotoi

Prorocentrum donghaiense

Prorocentrum minimum

Ulva compressa

Ulva ohnoi and Ulva prolifera. This method achieved a limit of detection (LOD) of 0.5 ng of genomic DNA (orders of magnitude of the deci-nanogram range) in the tested algae cultures. Altogether

230 field samples from ship ballast waters and seaport waters were used to evaluate the DNA microarray. The clinical sensitivity and specificity of the DNA microarray assay in detecting field samples were 96.4% and 90.9%

respectively

relative to conventional morphological methods. This indicated that this high-throughput

automatic

and specific method is well suited for the detection of algae in water samples.

关键词

Keywords

references

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