Isolation and characterization of bioactive fungi from shark Carcharodon carcharias’ gill with biopharmaceutical prospects

Yi Zhang; Jinyuan Han; Yan Feng; Jun Mu; Haiyan Bao; Andreas Kulik; Stephanie Grond

doi:10.1007/s00343-015-4187-6

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Isolation and characterization of bioactive fungi from shark Carcharodon carcharias’ gill with biopharmaceutical prospects

OriginalPaper | Updated：2023-05-16

- Isolation and characterization of bioactive fungi from shark Carcharodon carcharias’ gill with biopharmaceutical prospects
- Isolation and characterization of bioactive fungi from shark Carcharodon carcharias’ gill with biopharmaceutical prospects
- 海洋湖沼学报（英文） 2016年34卷第1期页码：186-199
- Affiliations：
  
  1. College of Food Science and Technology Guangdong Ocean University Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety Key Laboratory of Advanced Processing of Aquatic Products of Guangdong Higher Education Institution
  2. School of Environmental and Chemical Engineering Dalian Jiaotong University
  3. School of Life Science and Biotechnology Dalian University of Technology
  4. Institute of Organic Chemistry University of Tübingen
  5. Marine Science and Technology College Zhejiang Ocean University
  6. Inter - Faculty Institute for Microbiology and Infectious Medicine University of Tübingen
- Author bio：
- Funds：
  
  Supported by the National Natural Science Foundation of China (No. 20902009), the National Science Foundation for Post-Doctoral Scientists of China (Nos. 2011M500051, 2012T50258), the YangFan Scarce Top Talent Project of Guangdong Province (to ZHANG Yi), the Program for Scientific Research Start-up Funds of Guangdong Ocean University (GDOU) (to ZHANG Yi), and the Natural Science Research Project of GDOU (No. C14519)
- DOI：10.1007/s00343-015-4187-6
  中图分类号：
- 网络首发：2015-06-04，
  
  纸质出版：2016
- Accepted：
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Isolation and characterization of bioactive fungi from shark Carcharodon carcharias’ gill with biopharmaceutical prospects[J]. 海洋湖沼学报（英文）, 2016,34(1):186-199.

Zhang, Y., Han, J., Feng, Y. et al. Isolation and characterization of bioactive fungi from shark Carcharodon carcharias’ gill with biopharmaceutical prospects., Chin. J. Ocean. Limnol. 34, 186–199 (2016). https://doi.org/10.1007/s00343-015-4187-6
Isolation and characterization of bioactive fungi from shark Carcharodon carcharias’ gill with biopharmaceutical prospects[J]. 海洋湖沼学报（英文）, 2016,34(1):186-199. DOI： 10.1007/s00343-015-4187-6.

Zhang, Y., Han, J., Feng, Y. et al. Isolation and characterization of bioactive fungi from shark Carcharodon carcharias’ gill with biopharmaceutical prospects., Chin. J. Ocean. Limnol. 34, 186–199 (2016). https://doi.org/10.1007/s00343-015-4187-6 DOI：

摘要

Abstract

Until recently

little was known about the fungi found in shark gills and their biomedicinal potential. In this article

we described the isolation

bioactivity

diversity

and secondary metabolites of bioactive fungi from the gill of a shark (Carcharodon carcharias). A total of 115 isolates were obtained and grown in 12 culture media. Fifty-eight of these isolates demonstrated significant activity in four antimicrobial

pesticidal

and cytotoxic bioassay models. Four randomly selected bioactive isolates inhibited human cancer cell proliferation during re-screening. These active isolates were segregated into 6 genera using the internal transcribed spacer-large subunit (ITS-LSU) rDNA-sequence BLAST comparison. Four genera

Penicillium

Aspergillus

Mucor

and Chaetomium were the dominant taxa. A phylogenic tree illustrated their intergenera and intragenera genetic diversity. HPLC-DAD-HRMS analysis and subsequent database searching revealed that nine representative strains produced diverse bioactive compound profiles. These results detail the broad range of bioactive fungi found in a shark’s gills

revealing their biopharmaceutical potential. To the best of our knowledge

this is the first study characterizing shark gill fungi and their bioactivity.

关键词

Keywords

references

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