The Clock gene clone and its circadian rhythms in Pelteobagrus vachelli

Chuanjie Qin; Ting Shao

doi:10.1007/s00343-015-4167-x

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The Clock gene clone and its circadian rhythms in Pelteobagrus vachelli

OriginalPaper | Updated：2023-05-16

- The Clock gene clone and its circadian rhythms in Pelteobagrus vachelli
- The Clock gene clone and its circadian rhythms in Pelteobagrus vachelli
- 海洋湖沼学报（英文） 2015年33卷第3期页码：597-603
- Affiliations：
  
  College of Life Sciences Neijiang Normal University
- Author bio：
- Funds：
  
  Supported by the National Natural Science Foundation of China (No. 31402305), the Educational Commission of Sichuan Province of China (No. 14ZA0249), the Key Technologies R&D Program of Neijiang, Sichuan, China (No. 12108), and the College Students’ Scientific Research Project of Neijiang Normal University (Nos. 13NSD-66, 13NSD-77)
- DOI：10.1007/s00343-015-4167-x
  中图分类号：
- 网络首发：2015-01-30，
  
  纸质出版：2015
- Accepted：
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The Clock gene clone and its circadian rhythms in Pelteobagrus vachelli[J]. 海洋湖沼学报（英文）, 2015,33(3):597-603.

Qin, C., Shao, T. The Clock gene clone and its circadian rhythms in Pelteobagrus vachelli, ., Chin. J. Ocean. Limnol. 33, 597–603 (2015). https://doi.org/10.1007/s00343-015-4167-x
The Clock gene clone and its circadian rhythms in Pelteobagrus vachelli[J]. 海洋湖沼学报（英文）, 2015,33(3):597-603. DOI： 10.1007/s00343-015-4167-x.

Qin, C., Shao, T. The Clock gene clone and its circadian rhythms in Pelteobagrus vachelli, ., Chin. J. Ocean. Limnol. 33, 597–603 (2015). https://doi.org/10.1007/s00343-015-4167-x DOI：

摘要

Abstract

The Clock gene

a key molecule in circadian systems

is widely distributed in the animal kingdom. We isolated a 936-bp partial cDNA sequence of the Clock gene (Pva-clock) from the darkbarbel catfish Pelteobagrus vachelli that exhibited high identity with Clock genes of other species of fish and animals (65%–88%). The putative domains included a basic helix-loop-helix (bHLH) domain and two period-ARNT-single-minded (PAS) domains

which were also similar to those in other species of fish and animals. Pva-Clock was primarily expressed in the brain

and was detected in all of the peripheral tissues sampled. Additionally

the pattern of Pva-Clock expression over a 24-h period exhibited a circadian rhythm in the brain

liver and intestine

with the acrophase at zeitgeber time 21:35

23:00

and 23:23

respectively. Our results provide insight into the function of the molecular Clock of P. vachelli.

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references

Allada R, White N E, So W V, Hall J C, Rosbash M. 1998. A mutant Drosophila homolog of mammalian Clock disrupts circadian rhythms and transcription of period and timeless. Cell, 93(5): 791–804.

Antoch M P, Song E J, Chang A M, Vitaterna M H, Zhao Y L, Wilsbacher L D, Sangoram A M, King D P, Pinto L H, Takanashi J S. 1997. Functional identification of the mouse circadian Clock gene by transgenic BAC rescue. Cell, 89(4): 655–667.

Bell-Pedersen D, Cassone V M, Earnest D J, Golden S S, Hardin P E, Thomas T L, Zoran M J. 2005. Circadian rhythms from multiple oscillators: lessons from diverse organisms. Nat. Rev. Genet., 6(7): 544–556.

Cerdá-Reverter J M, Rodríguez G M, Zanuy S, Carrillo M, Larhammar D. 1998. Cloning of neuropeptide Y, peptide YY, and peptide Y from sea bass (Dicentrarchus labrax), a marine teleost. Annals of the New York Academy of Sciences, 839: 493–495.

Chang D C, McWatters H G, Williams J A, Gotter A L, Levine J D, Reppert S M. 2003. Constructing a feedback loop with circadian Clock molecules from the silkmoth, Antheraea pernyi. J. Biol. Chem., 278(40): 38 149–38 158.

Davie D, Minghetti M, Migaud H. 2009. Seasonal variations in Clock-gene expression in Atlantic salmon (Salmo salar). Chronobiol. Int., 26(3): 379–395.

Del Pozo A, Vera L M, Sánchez J A, Sánchez-Vázquez F J. 2012. Molecular cloning, tissue distribution and daily expression of cry1 and cry2 clock genes in European seabass (Dicentrarchus labrax). Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology, 163(3–4): 364–371.

Feliciano A, Vivas Y, de Pedro N, Delgado M J, Velarde E, Isorna E. 2011. Feeding time synchronizes Clock gene rhythmic expression in brain and liver of goldfish (Carassius auratus). J. Biol. Rhythms, 26(1): 24–33.

Hardin P E. 2005. The circadian timekeeping system of Drosophila. Curr. Biol., 15(17): 714–722.

Hardin P E. 2009. Molecular mechanisms of circadian timekeeping in Drosophila. Sleep and Biological Rhythms, 7(4): 235–242.

Hirota T, Fukada Y. 2004. Resetting mechanism of central and peripheral circadian clocks in mammals. Zoolog. Sci., 21(4): 359–368.

Kohsaka A, Bass J. 2007. A sense of time: how molecular clocks organize metabolism. Trends Endocrinol. Metab., 18(1): 4–11.

Li M, Chen L Q, Qin J G, Li E C, Yu N, Du Z Y. 2013. Growth performance, antioxidant status and immune response in darkbarbel catfish Pelteobagrus vachelli fed different PUFA/vitamin E dietary levels and exposed to high or low ammonia. Aquaculture, 406–407: 18–27.

Livak K J, Schmittgen T D. 2001. Analysis of relative gene expression data using real-time quantitative PCR and the 2−ΔΔCt Method. Methods, 25(4): 402–408.

Martín-Robles Á J, Isorna E, Whitmore D, Muñoz-Cueto J A, Pendón C. 2011. The clock gene Period3 in the nocturnal flatfish Solea senegalensis: molecular cloning, tissue expression and daily rhythms in central areas. Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology, 159(1): 7–15.

Montoya A, López-Olmeda J F, Yúfera M, Sánchez-Muros M J, Sánchez-Vázquez F J. 2010. Feeding time synchronises daily rhythms of behaviour and digestive physiology in gilthead seabream (Sparus aurata). Aquaculture, 306(1–4): 315–321.

Park J G, Park Y J, Sugama N, Kim S J, Takemura A. 2007. Molecular cloning and daily variations of the Period gene in a reef fish Siganus guttatus. J. Comp. Physiol. A, 193(4): 403–411.

Steeves T D L, King D P, Zhao Y L, Sangoram A M, Du F H, Bowcock A M, Moore R Y, Takahashi J S. 1999. Molecular cloning and characterization of the human CLOCK gene: expression in the suprachiasmatic nuclei. Genomics, 57(2): 189–200.

Stokkan K A, Yamazaki S, Tei H, Sakaki Y, Menaker M. 2001. Entrainment of the circadian clock in the liver by feeding. Science, 291(5503): 490–493.

Tamai T K, Carr A J, Whitmore D. 2005. Zebrafish circadian clocks: cells that see light. Biochem. Soc. Trans., 33(Pt5): 962–966.

Velarde E, Haque R, Iuvone P M, Azpeleta C, Alonso-Gómez A L, Delgado M J. 2009. Circadian clock genes of goldfish, Carassius auratus: cDNA cloning and rhythmic expression of Period and Cryptochrome transcripts in retina, liver, and gut. J. Biol. Rhythms, 24(2): 104–113.

Whitmore D, Foulkes N S, Strähle U, Sassone-Corsi P. 1998. Zebrafish Clock rhythmic expression reveals independent peripheral circadian oscillators. Nat. Neurosci., 1(8): 701–707.

Xue J P, Ai Q H, Mai K S, Xu W, Yang Y H, Liufu Z G. 2011. Effects of melamine on growth performance and skin color of darkbarbel catfish (Pelteobagrus vachelli). Aquaculture, 320(1–2): 142–146.

Yang J S, Dai Z M, Yang F, Yang W J. 2006. Molecular cloning of Clock cDNA from the prawn, Macrobrachium rosenbergii. Brain Res., 1067(1): 13–24.

Yoshimura T, Suzuki Y, Makino E, Suzuki T, Kuroiwa A, Matsuda Y, Namikawa T, Ebihara S. 2000. Molecular analysis of avian circadian Clock genes. Mol. Brain Res., 78(1–2): 207–215.

Young M W, Kay S A. 2001. Time zones: a comparative genetics of circadian clocks. Nat. Rev. Genet., 2(9): 702–715.

Yu W J, Hardin P E. 2006. Circadian oscillators of Drosophila and mammals. J. Cell. Sci., 119(Pt23): 4 793–4 795.

Zheng K K, Zhu X M, Han D, Yang Y X, Lei W, Xie S Q. 2010. Effects of dietary lipid levels on growth, survival and lipid metabolism during early ontogeny of Pelteobagrus vachelli larvae. Aquaculture, 299(1–4): 121–127.

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