Characterization and identification of enzyme-producing microflora isolated from the gut of sea cucumber Apostichopus japonicus

Fenghui Li; Fei Gao; Jie Tan; Chaojing Fan; Huiling Sun; Jingping Yan; Siqing Chen; Xiaojun Wang

doi:10.1007/s00343-015-4149-z

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Characterization and identification of enzyme-producing microflora isolated from the gut of sea cucumber Apostichopus japonicus

OriginalPaper | Updated：2023-05-16

- Characterization and identification of enzyme-producing microflora isolated from the gut of sea cucumber Apostichopus japonicus
- Characterization and identification of enzyme-producing microflora isolated from the gut of sea cucumber Apostichopus japonicus
- 海洋湖沼学报（英文） 2016年34卷第1期页码：153-162
- Affiliations：
  
  1. Yellow Sea Fisheries Research Institute Chinese Academy of Fishery Sciences
  2. College of Fisheries and Life Science Shanghai Ocean University
  3. Zhaoyuan Marine Environmental Monitoring Station
- Author bio：
- Funds：
  
  Supported by the National High Technology Research and Development Program of China (863 Program) (No. 2012AA10A412), the National Natural Science Foundation of China (No. 41106145), the National Key Technology R&D Program of China (No. 2011BAD13B02), the Science and Technology Development Planning Project of Shandong Province (No. 2012GGA06021), and the Key Laboratory of Mariculture & Stock Enhancement in North China’s Sea, Ministry of Agriculture, China (No. 2014-MSENC-KF-03)
- DOI：10.1007/s00343-015-4149-z
  中图分类号：
- 网络首发：2015-06-17，
  
  纸质出版：2016
- Accepted：
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Characterization and identification of enzyme-producing microflora isolated from the gut of sea cucumber Apostichopus japonicus[J]. 海洋湖沼学报（英文）, 2016,34(1):153-162.

Li, F., Gao, F., Tan, J. et al. Characterization and identification of enzyme-producing microflora isolated from the gut of sea cucumber Apostichopus japonicus, ., Chin. J. Ocean. Limnol. 34, 153–162 (2016). https://doi.org/10.1007/s00343-015-4149-z
Characterization and identification of enzyme-producing microflora isolated from the gut of sea cucumber Apostichopus japonicus[J]. 海洋湖沼学报（英文）, 2016,34(1):153-162. DOI： 10.1007/s00343-015-4149-z.

Li, F., Gao, F., Tan, J. et al. Characterization and identification of enzyme-producing microflora isolated from the gut of sea cucumber Apostichopus japonicus, ., Chin. J. Ocean. Limnol. 34, 153–162 (2016). https://doi.org/10.1007/s00343-015-4149-z DOI：

摘要

Abstract

Gut microorganisms play an important role in the digestion of their host animals. The purpose of this research was to isolate and assess the enzyme-producing microbes from the Apostichopus japonicus gut. Thirty-nine strains that can produce at least one of the three digestive enzymes (protease

amylase

and cellulase) were qualitatively screened based on their extracellular enzyme-producing abilities. The enzyme-producing strains clustered into eight groups at the genetic similarity level of 100% by analyzing the restriction patterns of 16S rDNA amplified with Mbo I. Phylogenetic analysis revealed that 37 strains belonged to the genus Bacillus and two were members of the genus Virgibacillus. Enzyme-producing capability results indicate that the main enzyme-producing microflora in the A. japonicus gut was Bacillus

which can produce protease

amylase

and cellulase. Virgibacillus

however

can only produce protease. The high enzyme-producing capability of the isolates suggests that the gut microbiota play an important role in the sea cucumber digestive process.

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references

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