Expression responses of five cold tolerant related genes to two temperature dropping treatments in sea cucumber Apostichopus japonicus

Chengze Li; Yaqing Chang; Zhenguo Pang; Jun Ding; Nanjing Ji

doi:10.1007/s00343-015-4148-0

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Expression responses of five cold tolerant related genes to two temperature dropping treatments in sea cucumber Apostichopus japonicus

OriginalPaper | Updated：2023-05-16

- Expression responses of five cold tolerant related genes to two temperature dropping treatments in sea cucumber Apostichopus japonicus
- Expression responses of five cold tolerant related genes to two temperature dropping treatments in sea cucumber Apostichopus japonicus
- 海洋湖沼学报（英文） 2015年33卷第2期页码：309-318
- Affiliations：
  
  Key Laboratory of Mariculture and Stock Enhancement in North China’s Sea Ministry of Agriculture Dalian Ocean University
- Author bio：
- Funds：
  
  Supported by the National High Technology Research and Development Program of China (863 Program) (No. 2012AA10A412) and the National Natural Science Foundation of China (No. 31072230)
- DOI：10.1007/s00343-015-4148-0
  中图分类号：
- 网络首发：2014-12-10，
  
  纸质出版：2015
- Accepted：
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Expression responses of five cold tolerant related genes to two temperature dropping treatments in sea cucumber Apostichopus japonicus[J]. 海洋湖沼学报（英文）, 2015,33(2):309-318.

Li, C., Chang, Y., Pang, Z. et al. Expression responses of five cold tolerant related genes to two temperature dropping treatments in sea cucumber Apostichopus japonicus, ., Chin. J. Ocean. Limnol. 33, 309–318 (2015). https://doi.org/10.1007/s00343-015-4148-0
Expression responses of five cold tolerant related genes to two temperature dropping treatments in sea cucumber Apostichopus japonicus[J]. 海洋湖沼学报（英文）, 2015,33(2):309-318. DOI： 10.1007/s00343-015-4148-0.

Li, C., Chang, Y., Pang, Z. et al. Expression responses of five cold tolerant related genes to two temperature dropping treatments in sea cucumber Apostichopus japonicus, ., Chin. J. Ocean. Limnol. 33, 309–318 (2015). https://doi.org/10.1007/s00343-015-4148-0 DOI：

摘要

Abstract

Environmental conditions

including ambient temperature

play important roles in survival

growth development

and reproduction of the Japanese sea cucumber

Apostichopus japonicus. Low temperatures result in slowed growth and skin ulceration disease. In a previous study

we investigated the effect of low temperature on gene expression profiles in A. japonicus by suppression subtractive hybridization (SSH). Genes encoding Ferritin

Lysozyme

Hsp70

gp96

and AjToll were selected from a subtracted cDNA library of A. japonicus under acute cold stress. The transcriptional expression profiles of these genes were investigated in different tissues (coelomocyte

respiratory tree

intestine

longitudinal muscle) after exposure to acute and mild temperature dropping treatments. The results show that (1) the five cold-tolerance-related genes were found in all four tissues and the highest mRNA levels were observed in coelomocyte and respiratory tree; (2) under the temperature dropping treatments

three types of transcriptional regulation patterns were observed: primary suppression followed by up-regulation at −2°C

suppressed expression throughout the two treatments

and more rarely an initial stimulation followed by suppression; and (3) gene expression suppression was more severe under acute temperature dropping than under mild temperature dropping treatment. The five cold-tolerance-related genes that were distributed mainly in coelomocyte and respiratory tissues were generally down-regulated by low temperature stress but an inverse up-regulation event was found at the extreme temperature (−2°C).

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references

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