Cloning, tissue distribution and effects of fasting on pituitary adenylate cyclase-activating polypeptide in largemouth bass

Shengjie Li; Linqiang Han; Junjie Bai; Dongmei Ma; Yingchun Quan; Jiajia Fan; Peng Jiang; Lingyun Yu

doi:10.1007/s00343-015-4081-2

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Cloning, tissue distribution and effects of fasting on pituitary adenylate cyclase-activating polypeptide in largemouth bass

OriginalPaper | Updated：2023-05-16

- Cloning, tissue distribution and effects of fasting on pituitary adenylate cyclase-activating polypeptide in largemouth bass
- Cloning, tissue distribution and effects of fasting on pituitary adenylate cyclase-activating polypeptide in largemouth bass
- 海洋湖沼学报（英文） 2015年33卷第2期页码：328-338
- Affiliations：
  
  Pearl River Fisheries Research Institute Chinese Academy of Fisheries Sciences Key Laboratory of Tropical & Subtropical Fishery Resource Application & Cultivation China Ministry of Agriculture
- Author bio：
- Funds：
  
  Supported by the National Natural Science Foundation of China (No. 31201985) and the National Key Technology R&D Program of China (No. 2012BAD26B03)
- DOI：10.1007/s00343-015-4081-2
  中图分类号：
- 网络首发：2014-11-06，
  
  纸质出版：2015
- Accepted：
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Cloning, tissue distribution and effects of fasting on pituitary adenylate cyclase-activating polypeptide in largemouth bass[J]. 海洋湖沼学报（英文）, 2015,33(2):328-338.

Li, S., Han, L., Bai, J. et al. Cloning, tissue distribution and effects of fasting on pituitary adenylate cyclase-activating polypeptide in largemouth bass., Chin. J. Ocean. Limnol. 33, 328–338 (2015). https://doi.org/10.1007/s00343-015-4081-2
Cloning, tissue distribution and effects of fasting on pituitary adenylate cyclase-activating polypeptide in largemouth bass[J]. 海洋湖沼学报（英文）, 2015,33(2):328-338. DOI： 10.1007/s00343-015-4081-2.

Li, S., Han, L., Bai, J. et al. Cloning, tissue distribution and effects of fasting on pituitary adenylate cyclase-activating polypeptide in largemouth bass., Chin. J. Ocean. Limnol. 33, 328–338 (2015). https://doi.org/10.1007/s00343-015-4081-2 DOI：

摘要

Abstract

Pituitary adenylate cyclase activating polypeptide (PACAP) has a wide range of biological functions. We cloned the full-length cDNAs encoding PACAP and PACAP-related peptide (PRP) from the brain of largemouth bass (Micropterus salmoides) and used real-time quantitative PCR to detect PRP-PACAP mRNA expression. The PRP-PACAP cDNA has two variants expressed via alternative splicing: a long form

which encodes both PRP and PACAP

and a short form

which encodes only PACAP. Sequence analysis results are consistent with a higher conservation of PACAP than PRP peptide sequences. The expression of PACAP-long and PACAP-short transcripts was highest in the forebrain

followed by the medulla

midbrain

pituitary

stomach

cerebellum

intestine

and kidney; however

these transcripts were either absent or were weakly expressed in the muscle

spleen

gill

heart

fatty tissue

and liver. The level of PACAP-short transcript expression was significantly higher than expression of the long transcript in the forebrain

cerebella

pituitary and intestine

but lower than that of the long transcript in the stomach. PACAP-long and PACAP-short transcripts were first detected at the blastula stage of embryogenesis

and the level of expression increased markedly between the muscular contraction stage and 3 d post hatch (dph). The expression of PACAP-long and PACAP-short transcripts decreased significantly in the brain following 4 d fasting compared with the control diet group. The down-regulation effect was enhanced as fasting continued. Conversely

expression levels increased significantly after 3 d of re-feeding. Our results suggest that PRP-PACAP acts as an important factor in appetite regulation in largemouth bass.

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Keywords

references

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