Use of species-specific PCR for the identification of 10 sea cucumber species

Jing Wen; Ling Zeng

doi:10.1007/s00343-015-4050-9

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Use of species-specific PCR for the identification of 10 sea cucumber species

OriginalPaper | Updated：2023-05-16

- Use of species-specific PCR for the identification of 10 sea cucumber species
- Use of species-specific PCR for the identification of 10 sea cucumber species
- 海洋湖沼学报（英文） 2014年32卷第6期页码：1257-1263
- Affiliations：
  
  1. Department of Biology Lingnan Normal University
  2. Department of Chemistry Lingnan Normal University
- Author bio：
- Funds：
  
  Supported by the National Natural Science Foundation of China (No. 31201999), the Natural Science Foundation of Guangdong Province, China (No. S2011040000463), the Foundation for Distinguished Young Talents in Higher Education of Guangdong, China (No. LYM11086), the Key Laboratory Program of Tropical Marine Bio-Resources and Ecology, Chinese Academy of Science (No. LMB111004), the China Spark Program (Nos. 2012GA780007, 2012GA780020, 2012GA780008), the National Students’ Innovation and Entrepreneurship Training Project (No. 201210579031), and the Zhanjiang Foundation for Science and Technology, China (Nos. 2011C3104009, 2011D0244, 2012C3102018)
- DOI：10.1007/s00343-015-4050-9
  中图分类号：
- 网络首发：2014-08-07，
  
  纸质出版：2014
- Accepted：
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Use of species-specific PCR for the identification of 10 sea cucumber species[J]. 海洋湖沼学报（英文）, 2014,32(6):1257-1263.

Wen, J., Zeng, L. Use of species-specific PCR for the identification of 10 sea cucumber species., Chin. J. Ocean. Limnol. 32, 1257–1263 (2014). https://doi.org/10.1007/s00343-015-4050-9
Use of species-specific PCR for the identification of 10 sea cucumber species[J]. 海洋湖沼学报（英文）, 2014,32(6):1257-1263. DOI： 10.1007/s00343-015-4050-9.

Wen, J., Zeng, L. Use of species-specific PCR for the identification of 10 sea cucumber species., Chin. J. Ocean. Limnol. 32, 1257–1263 (2014). https://doi.org/10.1007/s00343-015-4050-9 DOI：

摘要

Abstract

We developed a species-specific PCR method to identify species among dehydrated products of 10 sea cucumber species. Ten reverse species-specific primers designed from the 16S rRNA gene

in combination with one forward universal primer

generated PCR fragments of ca. 270 bp length for each species. The specificity of the PCR assay was tested with DNA of samples of 21 sea cucumber species. Amplification was observed in specific species only. The species-specific PCR method we developed was successfully applied to authenticate species of commercial products of dehydrated sea cucumber

and was proven to be a useful

rapid

and low-cost technique to identify the origin of the sea cucumber product.

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references

Anderson S C, Flemming J M, Watson R, Lotze H K. 2011. Serial exploitation of global sea cucumber fisheries. Fish Fish., 12: 317–339.

Asensio L, Gonzalez I, Rojas M, Garcia T, Martin R. 2009. PCR-based methodology for the authentication of grouper (Epinephelus marginatus) in commercial fish fillets. Food Control, 20: 618–622.

Bruckner A W. 2005. The recent status of sea cucumber fisheries in the continental United States of America. SPC Beche-de-Mer Info. Bull., 22: 39–46.

Chan W H, Ling K H, Shaw P C, Chiu S W, But P Pui-Hay. 2012. Application of FINS and multiplex PCR for detecting genuine abalone products. Food Control, 23: 137–142.

Chen C H, Hsieh C H, Hwang D F. 2012. Species identification of Cyprinidae fish in Taiwan by FINS and PCR-RFLP analysis. Food Control, 28: 240–245.

Espineira M, Vieites J M. 2012. Rapid method for controlling the correct labeling of products containing common octopus (Octopus vulgaris) and main substitute species (Eledone cirrhosa and Dosidicus gigas) by fast real-time PCR. Food Chem., 135: 2 439–2 444.

FAO. 2008. Sea Cucumbers: A Global Review of Fisheries and Trade. FAO Fisheries and Aquaculture Technical Paper, Rome, 516, Rome, Italy. p.1–317.

Fernandez-Tajes J, Freire R, Mendez J. 2010. A simple one-step PCR method for the identification between European and American razor clams species. Food Chem., 118: 995–998.

Fernandez-Tajes J, Mendez J. 2007. Identification of the razor clam species Ensis arcuatus, E. siliqua, E. directus, E. macha, and Solen marginatus using PCR-RFLP analysis of the 5S rDNA region. J. Agr. Food Chem., 55: 7 278–7 282.

Friedman K, Eriksson H, Tardy E, Pakoa K. 2011. Management of sea cucumber stocks: patterns of vulnerability and recovery of sea cucumber stocks impacted by fishing. Fish Fish., 12: 75–93.

FSA. 2012. Food Standards Agency, FSA, enforcement and regulation, food fraud. Available at http://www.food.gov.uk/enforcement/enforcework/foodfraud/. Accessed on 03.11.12.

Fumiere O, Dubois M, Baeten V, Holst C V, Berben G. 2006. Effective PCR detection of animal species in highly processed animal byproducts and compound feeds. Anal. Bioanal. Chem., 385: 1 045–1 054.

Haye P A, Segovia N I, Vera R, Gallardo M A, Gallardo-Escarate C. 2012. Authentication of commercialized crabmeat in Chile using DNA barcoding. Food Control, 25: 239–244.

Kerr A M, Janies D A, Clouse R M, Samyn Y, Kuszak J, Kim J. 2005. Molecular phylogeny of coral-Reef sea cucumbers (Holothuriidae: Aspidochirotida) based on 16S mitochondrial ribosomal DNA sequence. Mar. Biotechnol., 7: 53–60.

Kumar S, Nei M, Dudley J, Tamura K. 2008. MEGA: a biologist-centric software for evolutionary analysis of DNA and protein sequences. Brief Bioinform., 9: 299–306.

Lago F C, Vieites J M, Espineira M. 2012. Development of a FINS-based method for the identification of skates species of commercial interest. Food Control, 24: 38–43.

Lin W F, Hwang D F. 2008. Application of species-specific PCR for the identification of dried bonito product (Katsuobushi). Food Chem., 106: 390–396.

Lockley A K, Bardsley R G. 2000. DNA-based methods for food authentication. Trends Food Sci. Tech., 11: 67–77.

Marin A, Fujimoto T, Arai K. 2013. Rapid species identification of fresh and processed scallops by multiplex PCR. Food Control, 32: 472–476.

Palumbi S R, Martin A P, Romano S, McMillan W O, Stice L, Grabowski G. 1991. The Simple Fool’s Guide to PCR. HI: Department of Zoology Special Publication, University of Hawaii, Honolulu.

Pardo M A, Perez-Villareal B. 2004. Identification of commercial canned tuna species by restriction site analysis of mitochondrial DNA products obtained by nested primer PCR. Food Control, 86: 143–150.

Pascoal A, Barros-V J, Ortea I, Cepeda A, Gallardo J M, Calo-M P. 2011. Molecular identification of the black tiger shrimp (Penaeus monodon), the white leg shrimp (Litopenaeus vannamei) and the Indian white shrimp (Fenneropenaeus indicus) by PCR targeted to the 16S rRNA mtDNA. Food Chem., 125: 1 457–1 461.

Pascoal A, Ortea I, Gallardo J M, Canas B, Barros-V J, Calo-M P. 2012. Species identification of the Northern shrimp (Pandalus borealis) by polymerase chain reaction-restriction fragment length polymorphism and proteomic analysis. Anal. Biochem., 421: 56–67.

Pereira A M, Fernandez-Tajes J, Gaspar M B, Mendez J. 2012. Identification of the wedge clam Donax trunculus by a simple PCR technique. Food Control, 23: 268–270.

Soares S, Amaral J, Mafra I, Oliveira M. 2010. Quantitative detection of poultry meat adulteration with pork by a duplex PCR assay. Meat Sci., 85: 531–536.

Thompson J, Gibson T, Plewniak F, Jeanmougin F, Higgins D. 1997. The CLUSTAL-X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Research, 25: 4 876–4 882.

Uthicke S, Byrne M, Conand C. 2010. Genetic barcoding of commercial beche-de-mer species (Echinodermata: Holothuroidea). Molecular Ecology Resources, 10: 634–646.

Veneza I, Felipe B, Oliveira J, Silva R, Sampaio I, Schneider H, Gomes G. 2014. A barcode for the authentication of the snappers (Lutjanidae) of the western Atlantic: rDNA 5S or mitochondrial COI? Food Control, 38: 116–123.

Wen J, Hu C, Fan S. 2010a. Chemical composition and nutritional quality of sea cucumbers. J. Sci. Food Agric., 90: 2 469–2 474.

Wen J, Hu C, Zhang L, Fan S. 2011. Genetic identification of global commercial sea cucumber species on the basis of mitochondrial DNA sequences. Food Control, 22: 72–77.

Wen J, Hu C, Zhang L, Fan S. 2012. Identification of 11 sea cucumber species by species-specific PCR method. Food Control, 27: 380–384.

Wen J, Hu C, Zhang L, Luo P, Zhao Z, Fan S, Su T. 2010b. The application of PCR-RFLP and FINS for species identification used in sea cucumbers (Aspidochirotida: Stichopodidae) products from the market. Food Control, 21: 403–407.

Wen J, Hu C. 2010. Elemental composition of commercial sea cucumbers (holothurians). Food Addit. Contam. B, 3: 246–252.

Woolfe M, Gurung T, Walker M J. 2013. Can analytical chemists do molecular biology? A survey of the up-skilling of the UK official food control system in DNA food authenticity techniques. Food Control, 33: 385–392.

Xia S, Yang H, Li Y, Liu S, Zhou Y, Zhang L. 2012. Effects of different seaweed diets on growth, digestibility, and ammonia-nitrogen production of the sea cucumber Apostichopus japonicus (Selenka). Aquaculture, 338–341: 304–308.

Zou T, Li Z, Lv Y, Duan G, Wang C, Tang Q, Xue C. 2012. Rapid identification of sea cucumber species with multiplex-PCR. Food Control, 26: 58–62.

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