Molecular cloning, tissue expression of gene Muc2 in blunt snout bream Megalobrama amblycephala and regulation after re-feeding

Chunyu Xue; Bingwen Xi; Mingchun Ren; Jingjing Dong; Jun Xie; Pao Xu

doi:10.1007/s00343-015-4047-4

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Molecular cloning, tissue expression of gene Muc2 in blunt snout bream Megalobrama amblycephala and regulation after re-feeding

OriginalPaper | Updated：2023-05-16

- Molecular cloning, tissue expression of gene Muc2 in blunt snout bream Megalobrama amblycephala and regulation after re-feeding
- Molecular cloning, tissue expression of gene Muc2 in blunt snout bream Megalobrama amblycephala and regulation after re-feeding
- 海洋湖沼学报（英文） 2015年33卷第2期页码：291-298
- Affiliations：
  
  1. Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization Ministry of Agriculture Freshwater Fisheries Research Center Chinese Academy of Fishery Sciences
  2. Wuxi Fishery College Nanjing Agricultural University
  3. Hubei Collaborative Innovation Center for Freshwater Aquaculture
- Author bio：
- Funds：
  
  Supported by the National Natural Science Foundation of China (No. 31302222), the Earmarked Fund for China Agriculture Research System (No. CARS-46), the Freshwater Fisheries Research Center, CAFS Grant (Nos. 2013JBFM10, 2013JBFM03), and the Natural Sciences Foundation of Jiangsu Province (No. BK2011182)
- DOI：10.1007/s00343-015-4047-4
  中图分类号：
- 网络首发：2014-10-27，
  
  纸质出版：2015
- Accepted：
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Molecular cloning, tissue expression of gene Muc2 in blunt snout bream Megalobrama amblycephala and regulation after re-feeding[J]. 海洋湖沼学报（英文）, 2015,33(2):291-298.

Xue, C., Xi, B., Ren, M. et al. Molecular cloning, tissue expression of gene Muc2 in blunt snout bream Megalobrama amblycephala and regulation after re-feeding., Chin. J. Ocean. Limnol. 33, 291–298 (2015). https://doi.org/10.1007/s00343-015-4047-4
Molecular cloning, tissue expression of gene Muc2 in blunt snout bream Megalobrama amblycephala and regulation after re-feeding[J]. 海洋湖沼学报（英文）, 2015,33(2):291-298. DOI： 10.1007/s00343-015-4047-4.

Xue, C., Xi, B., Ren, M. et al. Molecular cloning, tissue expression of gene Muc2 in blunt snout bream Megalobrama amblycephala and regulation after re-feeding., Chin. J. Ocean. Limnol. 33, 291–298 (2015). https://doi.org/10.1007/s00343-015-4047-4 DOI：

摘要

Abstract

Mucins are important components of mucus

which form a natural

physical

biochemical and semipermeable mucosal layer on the epidermis of fish gills

skin

and the gastrointestinal tract. As the first step towards characterizing the function of Muc2

we cloned a partial Megalobrama amblycephala Muc2 cDNA of 2 175 bp

and analyzed its tissue-specific expression pattern by quantitative real-time PCR (qPCR). The obtained sequence comprised 41 bp 5′-untranslated region (5′-UTR)

2 134 bp open reading frame encoding a protein of 711 amino acids. BLAST searching and phylogenetic analysis showed that the predicted protein contained several common secreted mucin-module domains (VWD-C8-TIL-VWD-C8) and had high homology with mucins from other vertebrates. Among four candidate reference genes (β-Actin

RPI13α

RPII

18S) for the qPCR

RPII was chosen as an appropriate reference gene because of its lowest variation in different tissues. M. amblycephala Muc2 was mainly expressed in the intestine

in the order (highest to lowest) middle-intestine > fore-intestine > hind-intestine. Muc2 was expressed relatively poorly in other organs (brain

liver

kidney

spleen

skin and gill). Furthermore

after 20-days of starvation

M. amblycephala Muc2 expressions after refeeding for 0 h

3 h

16 h

3 d

and 10 d were significantly decreased in the three intestinal segments (P<0.05) at 16 h

and were then upregulated to near the initial level at 10 d.

关键词

Keywords

references

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